Localization of single copy DNA sequences on G-banded human chromosomes by in situ hybridization View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1981-07

AUTHORS

Mary E. Harper, Grady F. Saunders

ABSTRACT

Recombinant lambda bacteriophage clone H3 containing a human DNA segment of 14.9 kb present in one or two copies per haploid genome was isolated. In situ hybridization to human metaphase chromosomes of the 3H-labeled cloned DNA resulted in highly significant labeling (53% of cells) of band p36 of chromosome 1, such that 22% of all chromosomal grains were located on this region. Hybridization was dependent upon the presence of dextran sulfate in the hybridization mixture and was not affected by repetitive DNA competitor. These results demonstrate localization of a single copy sequence on human metaphase chromosomes. More... »

PAGES

431-439

Journal

TITLE

Chromosoma

ISSUE

3

VOLUME

83

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00327364

    DOI

    http://dx.doi.org/10.1007/bf00327364

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1004687909

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/7273954


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