Cloning of genomic sequences from the human Y chromosome after purification by dual beam flow sorting View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1983-08

AUTHORS

C. R. Müller, K. E. Davies, C. Cremer, G. Rappold, J. W. Gray, H. H. Ropers

ABSTRACT

Human Y chromosomes were purified by dual beam flow sorting from a human x Chinese hamster cell line retaining the Y as the only free human chromosome. DNA was extracted from the Y fraction and cloned into λgtWES.λB vector arms. More than 100 recombinant clones carrying human inserts have been characterised by Benton-Davis plaque screening and Southern blotting or in situ hybridisation. Several repetitive sequences were found to be predominantly located on the Y, whereas the majority also cross-hybridised with autosomal DNA. One repetitive clone gave a specific hybridisation signal with the X and the Y chromosome but not with autosomes. Preliminary evidence indicates that many clones contain single copy as well as repetitive sequences. However, no Y-specific single copy sequence has yet been identified. More... »

PAGES

110-115

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00327104

DOI

http://dx.doi.org/10.1007/bf00327104

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1019238507

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/6885043


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