The arrangement of chromosomes in nuclei of sperm from plethodontid salamanders View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1973-09

AUTHORS

H. C. Macgregor, Muriel H. Walker

ABSTRACT

Plethodontid salamanders have n = 13 or 14 large metacentric or sub-metacentric chromosomes. Sperm nuclei from Plethodon cinereus measure 72×1 μm. The nucleoprotein of spermatids is at first finely granular. In elongate spermatids it clumps into larger granules, which then fuse to form the compact nucleoprotein of the mature sperm. The nuclei of mature sperm are negatively birefringent with respect to their length. — 3H RNA complementary to high-density satellite DNA of centromeric heterochromatin in P. cinereus has been hybridized in-situ to spermatids and sperm, and its site of binding to these cells has been examined by autoradiography. Labelling of round spermatid nuclei is localized in a single patch. Elongate spermatid nuclei are labelled only over the rear quarter of the nucleus. Label over the nuclei of mature sperm is localized in a region extending 10–20 μm forwards from the rear of the nucleus. — In P. cinereus the ribosomal genes are located near the centromere on the short arm of chromosome 7. 3H ribosomal RNA hybridizes to a single patch in round spermatid nuclei. Elongate spermatid nuclei show label over a short segment of the rear half of the nucleus. In spermatids nearing maturity the labelled region is never more than 20 μm long. — These results indicate that in P. cinereus each chromosome is arranged in a U formation with its centromere at the base of the sperm nucleus, and its arms extended forwards along the length of the nucleus. — Among plethodontids, increase in ‘C’ value and corresponding increase in chromosome size is accompanied by increase in the length rather than the width of the sperm nucleus. — 3H ribosomal RNA hybridizes to a short segment in spermatid and sperm nuclei from Xenopus and Triturus. In these animals, the position of the labelled segment varies from sperm to sperm. More... »

PAGES

243-262

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00326179

DOI

http://dx.doi.org/10.1007/bf00326179

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1023374960


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Department of Zoology, School of Biological Sciences, University of Leicester, Leicester, England", 
          "id": "http://www.grid.ac/institutes/grid.9918.9", 
          "name": [
            "Department of Zoology, School of Biological Sciences, University of Leicester, Leicester, England"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Macgregor", 
        "givenName": "H. C.", 
        "id": "sg:person.01363500541.56", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01363500541.56"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Zoology, School of Biological Sciences, University of Leicester, Leicester, England", 
          "id": "http://www.grid.ac/institutes/grid.9918.9", 
          "name": [
            "Department of Zoology, School of Biological Sciences, University of Leicester, Leicester, England"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Walker", 
        "givenName": "Muriel H.", 
        "id": "sg:person.061351412.23", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.061351412.23"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/bf00399410", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037783087", 
          "https://doi.org/10.1007/bf00399410"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00325943", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032966480", 
          "https://doi.org/10.1007/bf00325943"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00586116", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030228319", 
          "https://doi.org/10.1007/bf00586116"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00285631", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044024080", 
          "https://doi.org/10.1007/bf00285631"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01625006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002610327", 
          "https://doi.org/10.1007/bf01625006"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00399409", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008281744", 
          "https://doi.org/10.1007/bf00399409"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00321139", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003097214", 
          "https://doi.org/10.1007/bf00321139"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00285630", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052303282", 
          "https://doi.org/10.1007/bf00285630"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00286157", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037264305", 
          "https://doi.org/10.1007/bf00286157"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1973-09", 
    "datePublishedReg": "1973-09-01", 
    "description": "Plethodontid salamanders have n = 13 or 14 large metacentric or sub-metacentric chromosomes. Sperm nuclei from Plethodon cinereus measure 72\u00d71 \u03bcm. The nucleoprotein of spermatids is at first finely granular. In elongate spermatids it clumps into larger granules, which then fuse to form the compact nucleoprotein of the mature sperm. The nuclei of mature sperm are negatively birefringent with respect to their length. \u2014 3H RNA complementary to high-density satellite DNA of centromeric heterochromatin in P. cinereus has been hybridized in-situ to spermatids and sperm, and its site of binding to these cells has been examined by autoradiography. Labelling of round spermatid nuclei is localized in a single patch. Elongate spermatid nuclei are labelled only over the rear quarter of the nucleus. Label over the nuclei of mature sperm is localized in a region extending 10\u201320 \u03bcm forwards from the rear of the nucleus. \u2014 In P. cinereus the ribosomal genes are located near the centromere on the short arm of chromosome 7. 3H ribosomal RNA hybridizes to a single patch in round spermatid nuclei. Elongate spermatid nuclei show label over a short segment of the rear half of the nucleus. In spermatids nearing maturity the labelled region is never more than 20 \u03bcm long. \u2014 These results indicate that in P. cinereus each chromosome is arranged in a U formation with its centromere at the base of the sperm nucleus, and its arms extended forwards along the length of the nucleus. \u2014 Among plethodontids, increase in \u2018C\u2019 value and corresponding increase in chromosome size is accompanied by increase in the length rather than the width of the sperm nucleus. \u2014 3H ribosomal RNA hybridizes to a short segment in spermatid and sperm nuclei from Xenopus and Triturus. In these animals, the position of the labelled segment varies from sperm to sperm.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/bf00326179", 
    "inLanguage": "en", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1312277", 
        "issn": [
          "0009-5915", 
          "1432-0886"
        ], 
        "name": "Chromosoma", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "3", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "40"
      }
    ], 
    "keywords": [
      "sperm nuclei", 
      "mature sperm", 
      "spermatid nuclei", 
      "ribosomal RNA", 
      "plethodontid salamanders", 
      "round spermatid nuclei", 
      "nuclei of sperm", 
      "sub-metacentric chromosomes", 
      "arrangement of chromosomes", 
      "ribosomal genes", 
      "chromosome size", 
      "satellite DNA", 
      "centromeric heterochromatin", 
      "chromosome 7", 
      "short arm", 
      "chromosomes", 
      "RNA", 
      "centromeres", 
      "spermatids", 
      "salamanders", 
      "sperm", 
      "short segments", 
      "elongate spermatids", 
      "single patch", 
      "nucleoprotein", 
      "large granules", 
      "nucleus", 
      "heterochromatin", 
      "plethodontids", 
      "Xenopus", 
      "cinereus", 
      "genes", 
      "Triturus", 
      "DNA", 
      "patches", 
      "corresponding increase", 
      "cells", 
      "region", 
      "granules", 
      "sites", 
      "maturity", 
      "animals", 
      "segments", 
      "segment varies", 
      "length", 
      "p.", 
      "rear half", 
      "formation", 
      "autoradiography", 
      "increase", 
      "arm", 
      "situ", 
      "size", 
      "rear quarters", 
      "arrangement", 
      "half", 
      "varies", 
      "labels", 
      "position", 
      "rear", 
      "results", 
      "base", 
      "respect", 
      "quarter", 
      "values", 
      "width", 
      "measures", 
      "forward"
    ], 
    "name": "The arrangement of chromosomes in nuclei of sperm from plethodontid salamanders", 
    "pagination": "243-262", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1023374960"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/bf00326179"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/bf00326179", 
      "https://app.dimensions.ai/details/publication/pub.1023374960"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-05-20T07:17", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220519/entities/gbq_results/article/article_132.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/bf00326179"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00326179'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00326179'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00326179'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00326179'


 

This table displays all metadata directly associated to this object as RDF triples.

169 TRIPLES      22 PREDICATES      103 URIs      86 LITERALS      6 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/bf00326179 schema:about anzsrc-for:06
2 anzsrc-for:0604
3 schema:author Nf9d79576ddc343cc8d1c0a922e97c1ff
4 schema:citation sg:pub.10.1007/bf00285630
5 sg:pub.10.1007/bf00285631
6 sg:pub.10.1007/bf00286157
7 sg:pub.10.1007/bf00321139
8 sg:pub.10.1007/bf00325943
9 sg:pub.10.1007/bf00399409
10 sg:pub.10.1007/bf00399410
11 sg:pub.10.1007/bf00586116
12 sg:pub.10.1007/bf01625006
13 schema:datePublished 1973-09
14 schema:datePublishedReg 1973-09-01
15 schema:description Plethodontid salamanders have n = 13 or 14 large metacentric or sub-metacentric chromosomes. Sperm nuclei from Plethodon cinereus measure 72×1 μm. The nucleoprotein of spermatids is at first finely granular. In elongate spermatids it clumps into larger granules, which then fuse to form the compact nucleoprotein of the mature sperm. The nuclei of mature sperm are negatively birefringent with respect to their length. — 3H RNA complementary to high-density satellite DNA of centromeric heterochromatin in P. cinereus has been hybridized in-situ to spermatids and sperm, and its site of binding to these cells has been examined by autoradiography. Labelling of round spermatid nuclei is localized in a single patch. Elongate spermatid nuclei are labelled only over the rear quarter of the nucleus. Label over the nuclei of mature sperm is localized in a region extending 10–20 μm forwards from the rear of the nucleus. — In P. cinereus the ribosomal genes are located near the centromere on the short arm of chromosome 7. 3H ribosomal RNA hybridizes to a single patch in round spermatid nuclei. Elongate spermatid nuclei show label over a short segment of the rear half of the nucleus. In spermatids nearing maturity the labelled region is never more than 20 μm long. — These results indicate that in P. cinereus each chromosome is arranged in a U formation with its centromere at the base of the sperm nucleus, and its arms extended forwards along the length of the nucleus. — Among plethodontids, increase in ‘C’ value and corresponding increase in chromosome size is accompanied by increase in the length rather than the width of the sperm nucleus. — 3H ribosomal RNA hybridizes to a short segment in spermatid and sperm nuclei from Xenopus and Triturus. In these animals, the position of the labelled segment varies from sperm to sperm.
16 schema:genre article
17 schema:inLanguage en
18 schema:isAccessibleForFree false
19 schema:isPartOf N1d7eb68992d2423cab7b626addde1d0c
20 Na30823891a374d4f8c20a9f9efb9bf15
21 sg:journal.1312277
22 schema:keywords DNA
23 RNA
24 Triturus
25 Xenopus
26 animals
27 arm
28 arrangement
29 arrangement of chromosomes
30 autoradiography
31 base
32 cells
33 centromeres
34 centromeric heterochromatin
35 chromosome 7
36 chromosome size
37 chromosomes
38 cinereus
39 corresponding increase
40 elongate spermatids
41 formation
42 forward
43 genes
44 granules
45 half
46 heterochromatin
47 increase
48 labels
49 large granules
50 length
51 mature sperm
52 maturity
53 measures
54 nuclei of sperm
55 nucleoprotein
56 nucleus
57 p.
58 patches
59 plethodontid salamanders
60 plethodontids
61 position
62 quarter
63 rear
64 rear half
65 rear quarters
66 region
67 respect
68 results
69 ribosomal RNA
70 ribosomal genes
71 round spermatid nuclei
72 salamanders
73 satellite DNA
74 segment varies
75 segments
76 short arm
77 short segments
78 single patch
79 sites
80 situ
81 size
82 sperm
83 sperm nuclei
84 spermatid nuclei
85 spermatids
86 sub-metacentric chromosomes
87 values
88 varies
89 width
90 schema:name The arrangement of chromosomes in nuclei of sperm from plethodontid salamanders
91 schema:pagination 243-262
92 schema:productId N790e53531de143ca9ecdfb485940f7ee
93 Ned1609c2e9f24a04ac0ecae84296000d
94 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023374960
95 https://doi.org/10.1007/bf00326179
96 schema:sdDatePublished 2022-05-20T07:17
97 schema:sdLicense https://scigraph.springernature.com/explorer/license/
98 schema:sdPublisher N8cb4724fedda44858dcd68683bb8eb36
99 schema:url https://doi.org/10.1007/bf00326179
100 sgo:license sg:explorer/license/
101 sgo:sdDataset articles
102 rdf:type schema:ScholarlyArticle
103 N1d7eb68992d2423cab7b626addde1d0c schema:volumeNumber 40
104 rdf:type schema:PublicationVolume
105 N790e53531de143ca9ecdfb485940f7ee schema:name dimensions_id
106 schema:value pub.1023374960
107 rdf:type schema:PropertyValue
108 N8cb4724fedda44858dcd68683bb8eb36 schema:name Springer Nature - SN SciGraph project
109 rdf:type schema:Organization
110 Na30823891a374d4f8c20a9f9efb9bf15 schema:issueNumber 3
111 rdf:type schema:PublicationIssue
112 Nc60064ef7bde4176af919fe7f9cd6fc3 rdf:first sg:person.061351412.23
113 rdf:rest rdf:nil
114 Ned1609c2e9f24a04ac0ecae84296000d schema:name doi
115 schema:value 10.1007/bf00326179
116 rdf:type schema:PropertyValue
117 Nf9d79576ddc343cc8d1c0a922e97c1ff rdf:first sg:person.01363500541.56
118 rdf:rest Nc60064ef7bde4176af919fe7f9cd6fc3
119 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
120 schema:name Biological Sciences
121 rdf:type schema:DefinedTerm
122 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
123 schema:name Genetics
124 rdf:type schema:DefinedTerm
125 sg:journal.1312277 schema:issn 0009-5915
126 1432-0886
127 schema:name Chromosoma
128 schema:publisher Springer Nature
129 rdf:type schema:Periodical
130 sg:person.01363500541.56 schema:affiliation grid-institutes:grid.9918.9
131 schema:familyName Macgregor
132 schema:givenName H. C.
133 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01363500541.56
134 rdf:type schema:Person
135 sg:person.061351412.23 schema:affiliation grid-institutes:grid.9918.9
136 schema:familyName Walker
137 schema:givenName Muriel H.
138 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.061351412.23
139 rdf:type schema:Person
140 sg:pub.10.1007/bf00285630 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052303282
141 https://doi.org/10.1007/bf00285630
142 rdf:type schema:CreativeWork
143 sg:pub.10.1007/bf00285631 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044024080
144 https://doi.org/10.1007/bf00285631
145 rdf:type schema:CreativeWork
146 sg:pub.10.1007/bf00286157 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037264305
147 https://doi.org/10.1007/bf00286157
148 rdf:type schema:CreativeWork
149 sg:pub.10.1007/bf00321139 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003097214
150 https://doi.org/10.1007/bf00321139
151 rdf:type schema:CreativeWork
152 sg:pub.10.1007/bf00325943 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032966480
153 https://doi.org/10.1007/bf00325943
154 rdf:type schema:CreativeWork
155 sg:pub.10.1007/bf00399409 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008281744
156 https://doi.org/10.1007/bf00399409
157 rdf:type schema:CreativeWork
158 sg:pub.10.1007/bf00399410 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037783087
159 https://doi.org/10.1007/bf00399410
160 rdf:type schema:CreativeWork
161 sg:pub.10.1007/bf00586116 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030228319
162 https://doi.org/10.1007/bf00586116
163 rdf:type schema:CreativeWork
164 sg:pub.10.1007/bf01625006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002610327
165 https://doi.org/10.1007/bf01625006
166 rdf:type schema:CreativeWork
167 grid-institutes:grid.9918.9 schema:alternateName Department of Zoology, School of Biological Sciences, University of Leicester, Leicester, England
168 schema:name Department of Zoology, School of Biological Sciences, University of Leicester, Leicester, England
169 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...