DNA of the Streptomyces phage SH10: Binding sites for Escherichia coli RNA polymerase and denaturation map View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1983-02

AUTHORS

Siegfried Klaus, Frank Vogel, Jasmin Gautschi, Margaretha Stålhammar-Carlemalm, Jürg Meyer

ABSTRACT

Escherichia coli RNA polymerase bound to Streptomyces phage SH10 DNA was visualized by electron microscopy. Six specific binding sites were observed at map units 53, 85, 93, 97, 98, and 99 on the physical map of the 48 kb long genome. Electron microscopy of partially denatured SH10 DNA revealed a characteristic melting pattern of A+T-rich regions around map units 1, 3, 48, 52, and 99. A comparison of the denaturation map with the RNA polymerase binding sites indicates that three binding sites are located in the most A+T-rich regions, two in other early melting regions and one in a segment of higher DNA helix stability. More... »

PAGES

21-26

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00326050

DOI

http://dx.doi.org/10.1007/bf00326050

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1005141936

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/6343784


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187 grid-institutes:grid.420264.6 schema:alternateName Zentralinstitut für Mikrobiologie and experimentelle Therapie, Akademie der Wissenschaften der DDR, DDR-69, Jena, Germany
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190 grid-institutes:grid.6612.3 schema:alternateName Abteilung Mikrobiologie, Biozentrum der Universität Basel, Klingelbergstr. 70, CH-4056, Basel, Switzerland
191 schema:name Abteilung Mikrobiologie, Biozentrum der Universität Basel, Klingelbergstr. 70, CH-4056, Basel, Switzerland
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