The glycerol kinase (GUT1) gene of Saccharomyces cerevisiae: cloning and characterization View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1993-07

AUTHORS

P. Pavlik, M. Simon, T. Schuster, H. Ruis

ABSTRACT

The GUT1 gene of Saccharomyces cerevisiae, encoding glycerol kinase, was cloned and sequenced. The cloned genomic DNA fragment contains an open reading frame potentially coding for a protein of 709 amino acids with homology to bacterial glycerol kinases (40.8% identity over 502 amino acids, and 42.1% identity over 496 amino acids, in comparison to the smaller E. coli and B. subtilis enzymes). Disruption of GUT1 showed that the gene is required for growth on glycerol, but not on glucose or ethanol media. No glycerol kinase activity was detected in the disruption mutant. According to enzyme activity and transcript analysis, synthesis of glycerol kinase is repressed by glucose, and derepression is ADR1-dependent. More... »

PAGES

21-25

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00324660

DOI

http://dx.doi.org/10.1007/bf00324660

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1035730531

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/8358828


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Amino Acid Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Base Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cloning, Molecular", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA, Fungal", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA-Binding Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fungal Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Expression Regulation, Fungal", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genes, Fungal", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Glycerol Kinase", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Restriction Mapping", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Saccharomyces cerevisiae", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Saccharomyces cerevisiae Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sequence Homology, Amino Acid", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transcription Factors", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Institut f\u00fcr Biochemie und Molekulare Zellbiologie der Universit\u00e4t Wien and Ludwig Boltzmann-Forschunsstelle f\u00fcr Biochemie, Dr. Bohrgasse 9, A-1030, Wien, Austria", 
          "id": "http://www.grid.ac/institutes/grid.10420.37", 
          "name": [
            "Institut f\u00fcr Biochemie und Molekulare Zellbiologie der Universit\u00e4t Wien and Ludwig Boltzmann-Forschunsstelle f\u00fcr Biochemie, Dr. Bohrgasse 9, A-1030, Wien, Austria"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Pavlik", 
        "givenName": "P.", 
        "id": "sg:person.01110100274.63", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01110100274.63"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institut f\u00fcr Biochemie und Molekulare Zellbiologie der Universit\u00e4t Wien and Ludwig Boltzmann-Forschunsstelle f\u00fcr Biochemie, Dr. Bohrgasse 9, A-1030, Wien, Austria", 
          "id": "http://www.grid.ac/institutes/grid.10420.37", 
          "name": [
            "Institut f\u00fcr Biochemie und Molekulare Zellbiologie der Universit\u00e4t Wien and Ludwig Boltzmann-Forschunsstelle f\u00fcr Biochemie, Dr. Bohrgasse 9, A-1030, Wien, Austria"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Simon", 
        "givenName": "M.", 
        "id": "sg:person.01173452260.46", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01173452260.46"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Forschungsinstitut f\u00fcr Molekular Pathologie, Dr. Bohrgasse 7, A-1030, Wien, Austria", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Forschungsinstitut f\u00fcr Molekular Pathologie, Dr. Bohrgasse 7, A-1030, Wien, Austria"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Schuster", 
        "givenName": "T.", 
        "id": "sg:person.0767733430.42", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0767733430.42"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institut f\u00fcr Biochemie und Molekulare Zellbiologie der Universit\u00e4t Wien and Ludwig Boltzmann-Forschunsstelle f\u00fcr Biochemie, Dr. Bohrgasse 9, A-1030, Wien, Austria", 
          "id": "http://www.grid.ac/institutes/grid.10420.37", 
          "name": [
            "Institut f\u00fcr Biochemie und Molekulare Zellbiologie der Universit\u00e4t Wien and Ludwig Boltzmann-Forschunsstelle f\u00fcr Biochemie, Dr. Bohrgasse 9, A-1030, Wien, Austria"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ruis", 
        "givenName": "H.", 
        "id": "sg:person.01230151060.28", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01230151060.28"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "1993-07", 
    "datePublishedReg": "1993-07-01", 
    "description": "The GUT1 gene of Saccharomyces cerevisiae, encoding glycerol kinase, was cloned and sequenced. The cloned genomic DNA fragment contains an open reading frame potentially coding for a protein of 709 amino acids with homology to bacterial glycerol kinases (40.8% identity over 502 amino acids, and 42.1% identity over 496 amino acids, in comparison to the smaller E. coli and B. subtilis enzymes). Disruption of GUT1 showed that the gene is required for growth on glycerol, but not on glucose or ethanol media. No glycerol kinase activity was detected in the disruption mutant. According to enzyme activity and transcript analysis, synthesis of glycerol kinase is repressed by glucose, and derepression is ADR1-dependent.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/bf00324660", 
    "inLanguage": "en", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1091193", 
        "issn": [
          "0172-8083", 
          "1432-0983"
        ], 
        "name": "Current Genetics", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1-2", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "24"
      }
    ], 
    "keywords": [
      "glycerol kinase", 
      "genomic DNA fragments", 
      "glycerol kinase gene", 
      "open reading frame", 
      "disruption mutant", 
      "glycerol kinase activity", 
      "reading frame", 
      "DNA fragments", 
      "kinase gene", 
      "kinase activity", 
      "transcript analysis", 
      "kinase", 
      "genes", 
      "amino acids", 
      "Saccharomyces", 
      "enzyme activity", 
      "derepression", 
      "GUT1", 
      "cloning", 
      "mutants", 
      "homology", 
      "protein", 
      "activity", 
      "fragments", 
      "disruption", 
      "growth", 
      "acid", 
      "glucose", 
      "characterization", 
      "ethanol medium", 
      "glycerol", 
      "frame", 
      "synthesis", 
      "medium", 
      "analysis", 
      "GUT1 gene", 
      "bacterial glycerol kinases", 
      "Disruption of GUT1"
    ], 
    "name": "The glycerol kinase (GUT1) gene of Saccharomyces cerevisiae: cloning and characterization", 
    "pagination": "21-25", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1035730531"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/bf00324660"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "8358828"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/bf00324660", 
      "https://app.dimensions.ai/details/publication/pub.1035730531"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2021-12-01T19:08", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20211201/entities/gbq_results/article/article_239.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/bf00324660"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00324660'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00324660'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00324660'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00324660'


 

This table displays all metadata directly associated to this object as RDF triples.

184 TRIPLES      21 PREDICATES      80 URIs      72 LITERALS      22 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/bf00324660 schema:about N11e92d7d83c34447a47b8cbf9f4170d7
2 N207f9f4ec98847a39e89b90364d184bc
3 N2273a71335204cd5afe1fdb76855e8cf
4 N37ecd03010894afe853a4a737e79ca92
5 N41b3696b345e4144b1d314be3fd1b6ef
6 N6be1c4e54f104fd382a2577da80ace21
7 N79c93063066648b8b5007634ea03902c
8 N8d335ab4fe9c4914937185a4b29cb828
9 N8ed36eecb8234036a696347f18b2ad96
10 Nce6014c4d7e2413896f48eed9588d40a
11 Ne13a2177cbf14f51a1bbc87fa4a25a7a
12 Ne8c9463817744ca2b95757424c5e31e0
13 Nef435c4967284b7988aa51b95d125927
14 Nfdf5227cef5e4d7f9c3833506d62dc62
15 Nfdf9d1ad47e743e5b2f9bc6c783ab485
16 anzsrc-for:06
17 anzsrc-for:0601
18 schema:author N9c4245031d864881a324bcaede4c5e12
19 schema:datePublished 1993-07
20 schema:datePublishedReg 1993-07-01
21 schema:description The GUT1 gene of Saccharomyces cerevisiae, encoding glycerol kinase, was cloned and sequenced. The cloned genomic DNA fragment contains an open reading frame potentially coding for a protein of 709 amino acids with homology to bacterial glycerol kinases (40.8% identity over 502 amino acids, and 42.1% identity over 496 amino acids, in comparison to the smaller E. coli and B. subtilis enzymes). Disruption of GUT1 showed that the gene is required for growth on glycerol, but not on glucose or ethanol media. No glycerol kinase activity was detected in the disruption mutant. According to enzyme activity and transcript analysis, synthesis of glycerol kinase is repressed by glucose, and derepression is ADR1-dependent.
22 schema:genre article
23 schema:inLanguage en
24 schema:isAccessibleForFree false
25 schema:isPartOf N8c352e444e4b4367a68b801393dd07be
26 N8f329dae73724cf1af8046dbd35f6136
27 sg:journal.1091193
28 schema:keywords DNA fragments
29 Disruption of GUT1
30 GUT1
31 GUT1 gene
32 Saccharomyces
33 acid
34 activity
35 amino acids
36 analysis
37 bacterial glycerol kinases
38 characterization
39 cloning
40 derepression
41 disruption
42 disruption mutant
43 enzyme activity
44 ethanol medium
45 fragments
46 frame
47 genes
48 genomic DNA fragments
49 glucose
50 glycerol
51 glycerol kinase
52 glycerol kinase activity
53 glycerol kinase gene
54 growth
55 homology
56 kinase
57 kinase activity
58 kinase gene
59 medium
60 mutants
61 open reading frame
62 protein
63 reading frame
64 synthesis
65 transcript analysis
66 schema:name The glycerol kinase (GUT1) gene of Saccharomyces cerevisiae: cloning and characterization
67 schema:pagination 21-25
68 schema:productId N400b5caba7fc49a88e1f7eed269ceb38
69 N9ae9f278b20e475cb645d2c04fbe25e8
70 Nbbe97b0e944548d5a7584b44639bb6b7
71 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035730531
72 https://doi.org/10.1007/bf00324660
73 schema:sdDatePublished 2021-12-01T19:08
74 schema:sdLicense https://scigraph.springernature.com/explorer/license/
75 schema:sdPublisher N7b134b7822ba49a0b4886ca54e5068b9
76 schema:url https://doi.org/10.1007/bf00324660
77 sgo:license sg:explorer/license/
78 sgo:sdDataset articles
79 rdf:type schema:ScholarlyArticle
80 N11e92d7d83c34447a47b8cbf9f4170d7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
81 schema:name Saccharomyces cerevisiae
82 rdf:type schema:DefinedTerm
83 N207f9f4ec98847a39e89b90364d184bc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
84 schema:name Glycerol Kinase
85 rdf:type schema:DefinedTerm
86 N2273a71335204cd5afe1fdb76855e8cf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
87 schema:name Cloning, Molecular
88 rdf:type schema:DefinedTerm
89 N37ecd03010894afe853a4a737e79ca92 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
90 schema:name DNA, Fungal
91 rdf:type schema:DefinedTerm
92 N400b5caba7fc49a88e1f7eed269ceb38 schema:name dimensions_id
93 schema:value pub.1035730531
94 rdf:type schema:PropertyValue
95 N41b3696b345e4144b1d314be3fd1b6ef schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
96 schema:name Gene Expression Regulation, Fungal
97 rdf:type schema:DefinedTerm
98 N4b68a955a44f44dc97be905543b4819d rdf:first sg:person.0767733430.42
99 rdf:rest N87db8ecccb0849f1abc31db153749214
100 N6be1c4e54f104fd382a2577da80ace21 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
101 schema:name Base Sequence
102 rdf:type schema:DefinedTerm
103 N748ba46ad83d47638d85c80ddf8509c6 rdf:first sg:person.01173452260.46
104 rdf:rest N4b68a955a44f44dc97be905543b4819d
105 N79c93063066648b8b5007634ea03902c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
106 schema:name Amino Acid Sequence
107 rdf:type schema:DefinedTerm
108 N7b134b7822ba49a0b4886ca54e5068b9 schema:name Springer Nature - SN SciGraph project
109 rdf:type schema:Organization
110 N87db8ecccb0849f1abc31db153749214 rdf:first sg:person.01230151060.28
111 rdf:rest rdf:nil
112 N8c352e444e4b4367a68b801393dd07be schema:issueNumber 1-2
113 rdf:type schema:PublicationIssue
114 N8d335ab4fe9c4914937185a4b29cb828 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
115 schema:name Genes, Fungal
116 rdf:type schema:DefinedTerm
117 N8ed36eecb8234036a696347f18b2ad96 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
118 schema:name Fungal Proteins
119 rdf:type schema:DefinedTerm
120 N8f329dae73724cf1af8046dbd35f6136 schema:volumeNumber 24
121 rdf:type schema:PublicationVolume
122 N9ae9f278b20e475cb645d2c04fbe25e8 schema:name doi
123 schema:value 10.1007/bf00324660
124 rdf:type schema:PropertyValue
125 N9c4245031d864881a324bcaede4c5e12 rdf:first sg:person.01110100274.63
126 rdf:rest N748ba46ad83d47638d85c80ddf8509c6
127 Nbbe97b0e944548d5a7584b44639bb6b7 schema:name pubmed_id
128 schema:value 8358828
129 rdf:type schema:PropertyValue
130 Nce6014c4d7e2413896f48eed9588d40a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
131 schema:name Saccharomyces cerevisiae Proteins
132 rdf:type schema:DefinedTerm
133 Ne13a2177cbf14f51a1bbc87fa4a25a7a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
134 schema:name Transcription Factors
135 rdf:type schema:DefinedTerm
136 Ne8c9463817744ca2b95757424c5e31e0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
137 schema:name Restriction Mapping
138 rdf:type schema:DefinedTerm
139 Nef435c4967284b7988aa51b95d125927 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
140 schema:name Sequence Homology, Amino Acid
141 rdf:type schema:DefinedTerm
142 Nfdf5227cef5e4d7f9c3833506d62dc62 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
143 schema:name Molecular Sequence Data
144 rdf:type schema:DefinedTerm
145 Nfdf9d1ad47e743e5b2f9bc6c783ab485 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
146 schema:name DNA-Binding Proteins
147 rdf:type schema:DefinedTerm
148 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
149 schema:name Biological Sciences
150 rdf:type schema:DefinedTerm
151 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
152 schema:name Biochemistry and Cell Biology
153 rdf:type schema:DefinedTerm
154 sg:journal.1091193 schema:issn 0172-8083
155 1432-0983
156 schema:name Current Genetics
157 schema:publisher Springer Nature
158 rdf:type schema:Periodical
159 sg:person.01110100274.63 schema:affiliation grid-institutes:grid.10420.37
160 schema:familyName Pavlik
161 schema:givenName P.
162 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01110100274.63
163 rdf:type schema:Person
164 sg:person.01173452260.46 schema:affiliation grid-institutes:grid.10420.37
165 schema:familyName Simon
166 schema:givenName M.
167 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01173452260.46
168 rdf:type schema:Person
169 sg:person.01230151060.28 schema:affiliation grid-institutes:grid.10420.37
170 schema:familyName Ruis
171 schema:givenName H.
172 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01230151060.28
173 rdf:type schema:Person
174 sg:person.0767733430.42 schema:affiliation grid-institutes:None
175 schema:familyName Schuster
176 schema:givenName T.
177 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0767733430.42
178 rdf:type schema:Person
179 grid-institutes:None schema:alternateName Forschungsinstitut für Molekular Pathologie, Dr. Bohrgasse 7, A-1030, Wien, Austria
180 schema:name Forschungsinstitut für Molekular Pathologie, Dr. Bohrgasse 7, A-1030, Wien, Austria
181 rdf:type schema:Organization
182 grid-institutes:grid.10420.37 schema:alternateName Institut für Biochemie und Molekulare Zellbiologie der Universität Wien and Ludwig Boltzmann-Forschunsstelle für Biochemie, Dr. Bohrgasse 9, A-1030, Wien, Austria
183 schema:name Institut für Biochemie und Molekulare Zellbiologie der Universität Wien and Ludwig Boltzmann-Forschunsstelle für Biochemie, Dr. Bohrgasse 9, A-1030, Wien, Austria
184 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...