Ultrastructure of spontaneous vaginal keratinization in hanging-drop organ culture (Balb/cCrgl MICE) View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1967-12

AUTHORS

Alvaro Cuadros, R. A. Cooper

ABSTRACT

The vaginal epithelium of castrate 8.5 week old mice undergoes spontaneous keratinization in the absence of estrogen when placed in hanging-drop organ cultures using chemically defined medium 199. The early stages of in vivo and in vitro keratinization have been shown to be similar and are characterized by marked increases in cytoplasmic ribosomes and filaments which correspond to known biochemical changes induced by estrogen in cells of the female genital tract. Similarly, there are early changes in nuclear chromatin and nucleoli in vitro indicative of active messenger-RNA and ribosomal-RNA synthesis. In the later stages of in vitro keratinization, however, there is, by electron microscopy, a failure of keratohyaline granule formation and incomplete filament aggregation. Thus, estrogen induced in vivo and spontaneous in vitro keratinization are not truly comparable. The changes occurring in the organ culture system used have, however, been shown to be reproducible and, at the light microscopic level, comparable to those previously described in other in vitro studies. The culture vessel used in the present study should offer certain advantages over other organ culture systems. More... »

PAGES

429-462

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00320861

DOI

http://dx.doi.org/10.1007/bf00320861

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1027551613

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/5700560


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Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00320861'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00320861'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00320861'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00320861'


 

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