Induction of G- and R-banding in human chromosomes by the demethylating agent S-adenosyl-l-homocysteine View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1992-07

AUTHORS

S. F. De Cabo, C. Sentís, J. Fernández-Piqueras

ABSTRACT

In this report we describe the procedure of growing human lymphocytes with the demethylating agent S-adenosyl-L-homocysteine (SAH). After this treatment, which is not toxic for cell survival, both R- and G-banding were obtained by new experimental procedures: R-bands have been directly demonstrated with the GC-specific fluorochrome chromomycin A3 without the necessity of any AT-specific counterstaining agent; simultaneous G-banding and active nucleolar organizer regions have been obtained by silver impregnation of chromosomes and subsequent Giemsa staining. These results suggest a possible relationship between local differences in DNA methylation and the determination of the banded chromosome structure. More... »

PAGES

499-501

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00316070

DOI

http://dx.doi.org/10.1007/bf00316070

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1017738918

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/1429010


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