In vivo import of Candida tropicalis hydratase-dehydrogenase-epimerase into peroxisomes of Candida albicans View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1990-06

AUTHORS

J. D. Aitchison, R. A. Rachubinski

ABSTRACT

We present a system for studying peroxisomal protein targeting in Candida. We have expressed the Candida tropicalis gene encoding hydratase-dehydrogenase-epimerase (HDE) in Candida albicans. Immunoblot analyses of C. albicans transformants demonstrate the presence of oleic-acid inducible HDE (100 kDa) in peroxisomes of transformed cells, but not of control cells. Peroxisomes isolated from transformed cells show increased β-hydroxyacyl-CoA dehydrogenase specific activity, indicating that HDE is imported into peroxisomes of C. albicans where it is enzymatically active. C. albicans provides a useful model for the study of protein targeting to peroxisomes in vivo. More... »

PAGES

481-486

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00313075

DOI

http://dx.doi.org/10.1007/bf00313075

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1020561361

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/2202524


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