Cloning the REC1 gene of Ustilago maydis View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1991-07

AUTHORS

David W. Holden, Ad Spanos, Naheed Kanuga, Geoffrey R. Banks

ABSTRACT

The REC1 gene of Ustilago maydis plays a key role in homologous recombination and the repair of damaged DNA. In order to understand the nature and functions of the gene product, the gene has been cloned by functional complementation. A 3.8 kb cloned fragment complements the pleiotropic mitotic phenotype of different rec1 alleles. It does not complement the UV sensitivity of two other sensitive mutants. Disruption of the chromosomal copy of the 1.566 kb open reading frame within this fragment reproduces the rec1 pleiotropic phenotype. Furthermore, in diploids this disrupted reading frame is unable to complement previously characterised rec1 alleles. More... »

PAGES

145-150

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00312777

DOI

http://dx.doi.org/10.1007/bf00312777

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1048115024

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/1934111


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