An effective transformation method for Hansenula polymorpha View Full Text


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Article Info

DATE

1990-08

AUTHORS

E. Berardi, D. Y. Thomas

ABSTRACT

We have developed a rapid, simple transformation procedure for intact cells of Hansenula polymorpha. It is a modification of the LiAc method and can yield 104–105 transformants/μg DNA. The use of stationaryphase cells, a high cell density per plate and a hear pulse at 50°C for 10 min are among major modifications of the original method. We have also found that, within the XhoI-SalI chromosomal fragment of Saccharomyces cerevisae containing the LEU2 gene, a sequence exists which supports autonomous replication of plasmid moleculles in H. polymorpha. More... »

PAGES

169-170

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00312606

DOI

http://dx.doi.org/10.1007/bf00312606

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1032254432


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