Photodynamic therapy of bladder cancer —uptake and phototoxicity of photosan in vitro View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1991-11

AUTHORS

K. Miller, E. Reich, A. Rück, R. Hautmann

ABSTRACT

The uptake of photosan and the intracellular sites of photoradiation-induced damage were investigated in vitro in bladder carcinoma cells and in normal bladder cells. Cells were examined by phase contrast, fluorescence and electron microscopy. The concentration of photosan, measured in microgram/10(6) cells, showed a good correlation to the incubation time. At all incubation times, control cells showed a lower uptake when compared with tumor cells. Following photodynamic therapy (PDT), phase-contrast microscopy revealed marked changes in tumor cells, whereas only minor effects could be detected at the cell membrane of the control cells. Following PDT, most of the investigated cells showed changes of the mitochondria and cytoplasma. These changes consisted of dissolution of the cristae, predominantly in the central part of the mitochondria. Twenty-four hours after PDT the shape of the mitochondria had changed markedly and the cristae were found to be completely destroyed. Moreover, the cystoplasma showed numerous vacuoles, and the number of mitochondria was decreased compared to non-treated cells. More... »

PAGES

353-356

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00310149

DOI

http://dx.doi.org/10.1007/bf00310149

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1016041403

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/1759328


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