Regeneration of peach plants from callus derived from immature embryos View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1985-06

AUTHORS

F. A. Hammerschlag, G. Bauchan, R. Scorza

ABSTRACT

Peach plants were repeatedly regenerated from immature embryos but not from callus derived from mature embryos. A white, nodular, highly regenerative callus was obtained when friable, primary callus from immature embryos was transferred from medium containing 4.5 μM 2,4-dichlorophenoxyacetic acid and 0.44 μM benzyladenine (BA) to media containing 0.27 μM α-naphthaleneacetic acid (NAA) and 2.2 μM BA. This callus retained its morphogenetic potential for a minimum of three subcultures. Green nodular callus, that lacked regenerative capacity, was produced from primary callus derived from mature embryos. Maximum regeneration of shoots occurred when highly regenerative callus was transferred to a medium in which the NAA concentration was reduced five times and the BA concentration was increased two times. Regenerated shoots were rooted in the dark on a medium containing 28.5 μM indoleacetic acid. Cytogenetic analysis of regenerated plants indicated that all plants were diploid, 2n = 2x = 16. Phenotypic evaluation of regenerated plants, grown under field conditions, is now in progress. More... »

PAGES

248-251

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00304907

DOI

http://dx.doi.org/10.1007/bf00304907

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1022487804

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24252917


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