Inheritance and expression of a transgene insert in an aneuploid tobacco line View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1994-07

AUTHORS

M. A. Matzke, E. A. Moscone, Y -D. Park, I. Papp, H. Oberkofler, F. Neuhuber, A. J. M. Matzke

ABSTRACT

A T-DNA locus comprising nptII, uidA and nos genes — all under the control of the nos promoter (this locus was designated K because it encodes resistance to Kanamycin) - was found to be inherited erratically in a transgenic tobacco line. This anomalous behavior was partially explained following a karyotype analysis of plants representing several generations: these plants were aneuploids, presumably for the K-containing chromosome. During four generations of sexual propagation, transgenic plants that were either trisomic or tetrasomic for the K-containing chromosome (i.e. 2n=49 or 2n=50, respectively) were obtained. The trisomic plants (2n=48+1) were virtually indistinguishable phenotypically from normal euploids (2n=4x=48), whereas the tetrasomic plants (2n=48+2) were smaller, had somewhat misshapen leaves and exhibited reduced fertility. Although the amount of NPTH protein in different trisomic (K--, KK-, KKK) and tetrasomic (KK--, KKK-) plants was generally consistent with a K dosage effect, the genetic behavior of each trisomic — with respect to segregation of KanR and marker gene activity in progeny — was unique and not completely explicable by invoking aneuploidy. Specifically, unexpected gains or losses of K could occur, suggesting the formation of double reductional gametes and/or frequent gene conversion at this locus. The susceptibility of K locus marker genes to trans-inactivation in the trisomic and tetrasomic lines was tested by crossing in partially homologous silencing loci. In all transgenotypes tested, the three K marker genes were sensitive to trans-silencing, which was accompanied by methylation in all copies of the nos promoter. In addition to this directed inactivation/methylation, the K locus could also undergo infrequent, spontaneous partial methylation, which produced stable epialleles. In most plants, however, the multiple copies of the nos promoter at this locus remained unmethylated and active through four generations in all transgenotypes examined. The significance of these results for irregular inheritance patterns, aneuploid syndromes and homology-dependent gene silencing is discussed. More... »

PAGES

471-485

References to SciGraph publications

  • 1987-12. Assaying chimeric genes in plants: The GUS gene fusion system in PLANT MOLECULAR BIOLOGY REPORTER
  • 1991-08. Reversible inactivation of a transgene in Arabidopsis thaliana in MOLECULAR GENETICS AND GENOMICS
  • 1993-11. Expression instability and genetic disorders in transgenicNicotiana plumbaginifolia L. plants in TRANSGENIC RESEARCH
  • 1987-05. T-DNA structure and gene expression in petunia plants transformed by Agrobacterium tumefaciens C58 derivatives in MOLECULAR GENETICS AND GENOMICS
  • 1990-07. Epigenetic changes in the expression of the maize A1 gene inPetunia hybrida: Role of numbers of integrated gene copies and state of methylation in MOLECULAR GENETICS AND GENOMICS
  • 1964-12. NOMENCLATURE FOR CENTROMERIC POSITION ON CHROMOSOMES in HEREDITAS
  • 1993-01. A variety of epistatic interactions can occur between partially homologous transgene loci brought together by sexual crossing in MOLECULAR GENETICS AND GENOMICS
  • 1994. Inactivation of Repeated Genes — DNA-DNA Interaction? in HOMOLOGOUS RECOMBINATION AND GENE SILENCING IN PLANTS
  • 1994-05. Susceptibility of transgene loci to homology-dependent gene silencing in MOLECULAR GENETICS AND GENOMICS
  • 1983-09. The plasticity of the plant genome—Is it a requirement for success? in PLANT MOLECULAR BIOLOGY REPORTER
  • 1994-05. Homology-dependent gene silencing in transgenic plants: epistatic silencing loci contain multiple copies of methylated transgenes in MOLECULAR GENETICS AND GENOMICS
  • 1993-09. Epigenetic repeat-induced gene silencing (RIGS) inArabidopsis in PLANT MOLECULAR BIOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00302260

    DOI

    http://dx.doi.org/10.1007/bf00302260

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1021882212

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/7808397


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    65 genes
    66 genetic behavior
    67 homology-dependent gene silencing
    68 infrequent
    69 inheritance
    70 inheritance pattern
    71 inserts
    72 karyotype analysis
    73 leaves
    74 lines
    75 loci
    76 loss
    77 marker gene activity
    78 marker genes
    79 methylation
    80 most plants
    81 multiple copies
    82 normal euploids
    83 nos genes
    84 nos promoter
    85 nptII
    86 partial methylation
    87 patterns
    88 plants
    89 progeny
    90 promoter
    91 propagation
    92 protein
    93 respect
    94 results
    95 segregation
    96 sexual propagation
    97 significance
    98 silencing
    99 stable epialleles
    100 susceptibility
    101 syndrome
    102 tetrasomic lines
    103 tetrasomic plants
    104 tobacco lines
    105 transgene insert
    106 transgenic plants
    107 transgenic tobacco lines
    108 trisomic plants
    109 uidA
    110 unexpected gains
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