Interphase flow-cytogenetics: Correlation of DNA fluorescence to aneuploidy in human fibroblast cultures View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1982-10

AUTHORS

P. S. Rabinovitch, G. M. Martin, H. Hoehn

ABSTRACT

Improvements in the accuracy of DNA content determination by flow cytometry might allow the detection of aneuploidy in interphase cells to serve as an adjunct to conventional cytogenetics. We compared alternate methods of staining and fluorescence standardization in analyses of human diploid and constitutionally aneuploid cultured fibroblast-like cells. Optimum results were obtained using the dye DAPI (4,6-diamidino-2-phenylindole) and an avian red blood cell standard. The standard error of the estimate of DNA content by this technique was 0.7%-0.96%, approaching more closely than previously the range necessary for reliable discrimination of numerical chromosomal aberrations. More... »

PAGES

246-249

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00296451

DOI

http://dx.doi.org/10.1007/bf00296451

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1039885759

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/7173867


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