Abundance of protein-bound sulfhydryl and bisulfide groups at chromosomal nucleolus organizing regions View Full Text


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Article Info

DATE

1980-02

AUTHORS

Charles H. C. M. Buys, Jan Osinga

ABSTRACT

Silver stainability of the chromosomal nucleolus organizing regions that contain the structural genes for ribosomal RNA can be abolished by proteolytic and oxidative treatments. Histone extraction has no effect. This indicates that reducing groups of non-histone chromosomal proteins are responsible for silver staining. Treatment with fluorescent sulfhydryl and disulfide specific reagents followed by silver staining demonstrates coincidence of silver dots and brightly fluorescent spots at the short arms of human acrocentric chromosomes where ribosomal RNA-genes are located. After treatment with cupric sulfite reagent in the presence of urea fluorescence and silver staining was no longer possible. Silver staining has been reported to be associated with ribosomal RNA-gene activity. Acrocentric chromosomes that are negative in silver staining also lack the brightly fluorescent spots. Therefore, we conclude that an abundance of protein-bound sulfhydryl and disulfide groups occur at nucleolar organizing regions with active genes. Differentially fluorescing spots could not be observed after staining with fluorescamine. So, either the sulfhydryl reagents used in this study are much more sensitive than fluorescamine to study protein distributions in cytological preparations, or our observations point to a local accumulation of some specific protein(s) rich in sulfhydryls. The presence of many sulfhydryl and disulfide groups at the nucleolus organizing regions seems suggestive of a great flexibility of protein(s) by transition of sulfhydryl groups to disulfide bridges and vice versa at these highly active regions of the genome. More... »

PAGES

1-11

References to SciGraph publications

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  • 1957-09. Thiols, Disulphides and Thiosulphates: Some New Reactions and Possibilities in Peptide and Protein Chemistry in NATURE
  • 1979-01. Cytological and histochemical studies on the mechanism of the selective silver staining of nucleolus organizer regions (NORs) in HISTOCHEMISTRY AND CELL BIOLOGY
  • 1959-11. Cytophotometrische Bestimmungen von proteingebundenen Thiolen in der Mitose und Interphase von HeLa-Zellen in ZEITSCHRIFT FÜR KREBSFORSCHUNG UND KLINISCHE ONKOLOGIE
  • 1979-01. Pattern of activity of nucleolus organizers during spermatogenesis in mammals as analyzed by silver-staining in CHROMOSOMA
  • 1975-02. Differential staining of the satellite regions of human acrocentric chromosomes in CELLULAR AND MOLECULAR LIFE SCIENCES
  • 1976-03. Detection of nucleolus organizer regions in chromosomes of human, chimpanzee, gorilla, orangutan and gibbon in CHROMOSOMA
  • 1976-01. An improved technique for selective silver staining of nucleolar organizer regions in human chromosomes in HUMAN GENETICS
  • 1979-01. Chromosome core structure revealed by silver staining in CHROMOSOMA
  • 1977-01. Activation of mouse ribosomal RNA genes at the 2-cell stage in HUMAN GENETICS
  • 1973. Fluorescence Cytochemical Studies of Chromosomes: Quantitative Applications of Fluorescein Mercuric Acetate in FLUORESCENCE TECHNIQUES IN CELL BIOLOGY
  • 1977-12. Visualization of ribosomal gene activity: Silver stains proteins associated with rRNA transcribed from oocyte chromosomes in CHROMOSOMA
  • 1975-03. Visualization of nucleolar organizer regions in mammalian chromosomes using silver staining in CHROMOSOMA
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00292037

    DOI

    http://dx.doi.org/10.1007/bf00292037

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1033014232

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/6154565


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