Combined mapping of AFLP and RFLP markers in barley View Full Text


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Article Info

DATE

1995-01

AUTHORS

Jörg Becker, Pieter Vos, Martin Kuiper, Francesco Salamini, Manfred Heun

ABSTRACT

AFLP marker technology allows efficient DNA fingerprinting and the analysis of large numbers of polymorphic restriction fragments on polyacrylamide gels. Using the doubled haploids from the F1 of the cross Proctor x Nudinka, 118 AFLP markers were mapped onto a barley (Hordeum vulgare L.) RFLP map, also including five microsatellite and four protein marker loci. The AFLP markers mapped to all parts of the barley chromosomes and filled in the gaps on barley chromosomes 2L, 4L and 6 in which no RFLP loci had been mapped. Interestingly, the AFLP markers seldom interrupted RFLP clusters, but grouped next to them. The combined map covers 1873 cM, with a total of 282 markers. The merging of AFLP and RFLP markers increased the total map length; 402 cM were added to the map at the tips of chromosomes or in regions corresponding to earlier gaps. Another 375 cM resulted from mapping AFLP markers near to RFLP clusters or in between non-clustered RFLP markers. More... »

PAGES

65-73

References to SciGraph publications

  • 1994-10. Crossover distribution in barley analysed through RFLP linkage data in THEORETICAL AND APPLIED GENETICS
  • 1994-02. Genetic Map Location of the Barley Developmental Mutant Liguleless in Relation to RFLP Markers in HEREDITAS
  • 1994-12. A 300 kilobase interval genetic map of rice including 883 expressed sequences in NATURE GENETICS
  • 1994-06. Distribution of RAPD Markers on a Linkage Map of Barley in HEREDITAS
  • 1994-09. Comparison of RFLP and RAPD markers to estimating genetic relationships within and among cruciferous species in THEORETICAL AND APPLIED GENETICS
  • 1994. RFLP maps of barley in DNA-BASED MARKERS IN PLANTS
  • 1994-01. A comparison of RAPD and isozyme analyses for determining the genetic relationships among Avena sterilis L. accessions in THEORETICAL AND APPLIED GENETICS
  • 1993-04. Genetic and physical mapping of barley telomeres in MOLECULAR GENETICS AND GENOMICS
  • 1995-02. Barley microsatellites: allele variation and mapping in PLANT MOLECULAR BIOLOGY
  • 1993-10. The distribution of RFLP markers on chromosome 2(2H) of barley in relation to the physical and genetic location of 5S rDNA in THEORETICAL AND APPLIED GENETICS
  • 1989-03. RFLP Mapping in Plant Breeding: New Tools for an Old Science in NATURE BIOTECHNOLOGY
  • 1993-07. A molecular, isozyme and morphological map of the barley (Hordeum vulgare) genome in THEORETICAL AND APPLIED GENETICS
  • 1991-12. Construction of an RFLP map of barley in THEORETICAL AND APPLIED GENETICS
  • 1994-03. Conservation of Genome Structure Between Rice and Wheat in NATURE BIOTECHNOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00290237

    DOI

    http://dx.doi.org/10.1007/bf00290237

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1044072010

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/8552035


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