Karyotyping and identification of human chromosome polymorphisms by single fluorochrome flow cytometry View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1984-04

AUTHORS

D. K. Green, Judith A. Fantes, Karin E. Buckton, J. K. Elder, Patricia Malloy, A. Carothers, H. J. Evans

ABSTRACT

Frequency distributions of fluorescence intensity of ethidium bromide stained human chromosomes from nine phenotypically normal males are cross correlated and autocorrelated following repeated flow cytometric measurements. It is shown that each individual donor produces a fluorescence profile which is both visually and numerically different from those of other individuals in the set. The wide variety of chromosome heteromorphisms which occur to varying degrees for chromosomes 1, 9, 13, 14, 15, 16, 21, 22 and Y give rise to the uniqueness of a given fluorescence profile. Estimates of chromosome heteromorphisms for each individual in the set were made and then compared with parallel results obtained from inspection of Q-banded and C-banded conventional metaphase preparations. Fluorescence profiles identifiable with each individual were also obtained for Hoechst 33258 stained chromosomes. More... »

PAGES

143-146

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00286588

DOI

http://dx.doi.org/10.1007/bf00286588

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1041410491

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/6714973


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