Ontology type: schema:ScholarlyArticle
1994-09
AUTHORSMathilde Grelon, Françoise Budar, Sandrine Bonhomme, Georges Pelletier
ABSTRACTTranscription of a putative mitochondrial gene (orf138) has previously been correlated with Ogura cytoplasmic male-sterility (CMS) in rapeseed cybrids. In this paper, studies performed on a Brassica cybrid with a different organization of the orf138 locus confirm this association. We also show that mitochondria isolated from male-sterile rapeseed plants synthesize a polypeptide of 19 kDa, which is absent in fertile revertants. Antibodies against a glutathione S-transferase-ORF138 fusion protein were raised to establish that this 19 kDa polypeptide is the product of orf138. The anti-ORF138 serum was used to demonstrate that the orf138 translation product occurs only in sterile cybrids and co-purifies with the mitochondrial membrane fraction. More... »
PAGES540-547
http://scigraph.springernature.com/pub.10.1007/bf00284202
DOIhttp://dx.doi.org/10.1007/bf00284202
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PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/8208245
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