Hypomethylated sequences: Characterization of the duplicate soybean genome View Full Text


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Article Info

DATE

1994-11

AUTHORS

Tong Zhu, James M. Schupp, Arnold Oliphant, Paul Keim

ABSTRACT

Soybean is believed to be a diploidized tetraploid generated from an allotetraploid ancestor. In this study, we used hypomethylated genomic DNA as a source of probes to investigate the genomic structure and methylation patterns of duplicated sequences. Forty-five genomic clones from Phaseolus vulgaris and 664 genomic clones from Glycine max were used to examine the duplicated regions in the soybean genome. Southern analysis of genomic DNA using probes from both sources revealed that greater than 15% of the hypomethylated genomic regions were only present once in the soybean genome. The remaining ca. 85% of the hypomethylated regions comprise duplicated or middle repetitive DNA sequences. If only the ratio of single to duplicate probe patterns is considered, it appears that 25% of the single-copy sequences have been lost. By using a subset of probes that only detected duplicated sequences, we examined the methylation status of the homeologous genomes with the restriction enzymes MspI and HpaII. We found that in all cases both copies of these regions were hypomethylated, although there were examples of low-level methylation. It appears that duplicate sequences are being eliminated in the diploidization process. Our data reveal no evidence that duplicated sequences are being "silenced" by inactivation correlated with methylation patterns. More... »

PAGES

638-645

References to SciGraph publications

  • 1991-06. DNA restriction fragment length polymorphisms correlate with isozyme diversity in Phaseolus vulgaris L. in THEORETICAL AND APPLIED GENETICS
  • 1991-10. Reactivation of a silent Ac following tissue culture is associated with heritable alterations in its methylation pattern in MOLECULAR GENETICS AND GENOMICS
  • 1991-08. Nuclear DNA content of some important plant species in PLANT MOLECULAR BIOLOGY REPORTER
  • 1978-02. DNA sequence organization in the soybean plant in BIOCHEMICAL GENETICS
  • 1993-03. DNaseI-sensitive and undermethylated rDNA is preferentially expressed in a maize hybrid in PLANT MOLECULAR BIOLOGY
  • 1990-01. Methylated and undermethylated rDNA repeats are interspersed at random in two higher plant species in PLANT MOLECULAR BIOLOGY
  • 1981-08. Sequence specificity of methylation in higher plant DNA in NATURE
  • 1988-08. Tomato genome is comprised largely of fast-evolving, low copy-number sequences in MOLECULAR GENETICS AND GENOMICS
  • 1988-10. DNA methylation of the maize transposable element Ac interferes with its transcription in MOLECULAR GENETICS AND GENOMICS
  • 1993-06. Physical mapping of a region in the soybean (Glycine max) genome containing duplicated sequences in PLANT MOLECULAR BIOLOGY
  • 1986-05. CpG-rich islands and the function of DNA methylation in NATURE
  • 1992-11. Evaluation of soybean RFLP marker diversity in adapted germ plasm in THEORETICAL AND APPLIED GENETICS
  • 1993-01. Correlation between the size of the intergenic regulatory region, the status of cytosine methylation of rRNA genes and nucleolar expression in wheat in MOLECULAR GENETICS AND GENOMICS
  • 1989-02. Where are the diploid (2n=2x=20) genome donors ofGlycine Willd. (Leguminosae, Papilionoideae)? in EUPHYTICA
  • 1988-06. Restriction fragment length polymorphisms as genetic markers in soybean, Glycine max (L.) merrill in THEORETICAL AND APPLIED GENETICS
  • 1986-11. Two soybean ribulose-1,5-bisphosphate carboxylase small subunit genes share extensive homology even in distant flanking sequences in PLANT MOLECULAR BIOLOGY
  • 1983-09. The frequency and distribution of methylatable DNA sequences in leguminous plant protein coding genes in JOURNAL OF MOLECULAR EVOLUTION
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00282754

    DOI

    http://dx.doi.org/10.1007/bf00282754

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/7969033


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