Reversible inactivation of a transgene in Arabidopsis thaliana View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1991-08

AUTHORS

Ortrun Mittelsten Scheid, Jerzy Paszkowski, Ingo Potrykus

ABSTRACT

Fifty percent of Arabidopsis thaliana plants transgenic for a hygromycin resistance gene failed to transmit the resistance phenotype to the progeny. The complete transgene was, however, inherited in all cases according to Mendelian laws as observed by Southern analysis. This discrepancy between genotype and phenotype was the result of a reduced level of transcript in the sensitive transformants. The gene inactivation occurred in plants with multicopy integration of the foreign DNA. No definite correlation was found between gene inactivity and methylation of cytidine residues in the transgene sequence. Explants from several sensitive transformed plants regained a low level of hygromycin resistance on callus induction medium. Subsequent generations obtained by self-pollination were sensitive. In contrast, spontaneous restoration of hygromycin tolerance was observed in seedlings originating from out-crosses with wild-type plants or a different sensitive transformant. A reduction of the copy number was not a prerequisite for spontaneous reactivation. The resistance was often lost again in the next generation. Inactivation and reactivation of the transgene are therefore reversible. More... »

PAGES

104-112

References to SciGraph publications

  • 1987-06. Agrobacterium-mediated transformation of germinating seeds of Arabidopsis thaliana: A non-tissue culture approach in MOLECULAR GENETICS AND GENOMICS
  • 1989-03. DNA methylation of embryogenic carrot cell cultures and its variations as caused by mutation, differentiation, hormones and hypomethylating drugs in THEORETICAL AND APPLIED GENETICS
  • 1990-07. Epigenetic changes in the expression of the maize A1 gene inPetunia hybrida: Role of numbers of integrated gene copies and state of methylation in MOLECULAR GENETICS AND GENOMICS
  • 1989-05. Efficient transformation of Arabidopsis thaliana using direct gene transfer to protoplasts in MOLECULAR GENETICS AND GENOMICS
  • 1986-07. Clones from a shooty tobacco crown gall tumor II: irregular T-DNA structures and organization, T-DNA methylation and conditional expression of opine genes in PLANT MOLECULAR BIOLOGY
  • 1985-05. Molecular and general genetics of a hybrid foreign gene introduced into tobacco by direct gene transfer in MOLECULAR GENETICS AND GENOMICS
  • 1981-10. Mendelian transcmission of genes introduced into plants by the Ti plasmids of Agrobacterium tumefaciens in MOLECULAR GENETICS AND GENOMICS
  • 1991-02. Arabidopsis thaliana: protocol for plant regeneration from protoplasts in PLANT CELL REPORTS
  • 1988-12. High efficiency Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana leaf and cotyledon explants in PLANT CELL REPORTS
  • 1987-04. High meiotic stability of a foreign gene introduced into tobacco by Agrobacterium-mediated transformation in MOLECULAR GENETICS AND GENOMICS
  • 1991-02. Direct gene transfer to protoplasts of Arabidopsis thaliana in PLANT CELL REPORTS
  • 1986-12. Transposase activity of the Ac controlling element in maize is regulated by its degree of methylation in MOLECULAR GENETICS AND GENOMICS
  • 1984-04. The DNA of Arabidopsis thaliana in MOLECULAR GENETICS AND GENOMICS
  • 1984-11. Changes in T-DNA methylation and expression are associated with phenotypic variation and plant regeneration in a crown gall tumor line in MOLECULAR GENETICS AND GENOMICS
  • 1988-03. DNA methylation and tissue-specific transcription of the storage protein genes of maize in PLANT MOLECULAR BIOLOGY
  • Journal

    TITLE

    Molecular Genetics and Genomics

    ISSUE

    1-2

    VOLUME

    228

    Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00282454

    DOI

    http://dx.doi.org/10.1007/bf00282454

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1024311517

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/1886604


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