Reversible inactivation of a transgene in Arabidopsis thaliana View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1991-08

AUTHORS

Ortrun Mittelsten Scheid, Jerzy Paszkowski, Ingo Potrykus

ABSTRACT

Fifty percent of Arabidopsis thaliana plants transgenic for a hygromycin resistance gene failed to transmit the resistance phenotype to the progeny. The complete transgene was, however, inherited in all cases according to Mendelian laws as observed by Southern analysis. This discrepancy between genotype and phenotype was the result of a reduced level of transcript in the sensitive transformants. The gene inactivation occurred in plants with multicopy integration of the foreign DNA. No definite correlation was found between gene inactivity and methylation of cytidine residues in the transgene sequence. Explants from several sensitive transformed plants regained a low level of hygromycin resistance on callus induction medium. Subsequent generations obtained by self-pollination were sensitive. In contrast, spontaneous restoration of hygromycin tolerance was observed in seedlings originating from out-crosses with wild-type plants or a different sensitive transformant. A reduction of the copy number was not a prerequisite for spontaneous reactivation. The resistance was often lost again in the next generation. Inactivation and reactivation of the transgene are therefore reversible. More... »

PAGES

104-112

References to SciGraph publications

  • 1987-06. Agrobacterium-mediated transformation of germinating seeds of Arabidopsis thaliana: A non-tissue culture approach in MOLECULAR GENETICS AND GENOMICS
  • 1989-03. DNA methylation of embryogenic carrot cell cultures and its variations as caused by mutation, differentiation, hormones and hypomethylating drugs in THEORETICAL AND APPLIED GENETICS
  • 1990-07. Epigenetic changes in the expression of the maize A1 gene inPetunia hybrida: Role of numbers of integrated gene copies and state of methylation in MOLECULAR GENETICS AND GENOMICS
  • 1989-05. Efficient transformation of Arabidopsis thaliana using direct gene transfer to protoplasts in MOLECULAR GENETICS AND GENOMICS
  • 1986-07. Clones from a shooty tobacco crown gall tumor II: irregular T-DNA structures and organization, T-DNA methylation and conditional expression of opine genes in PLANT MOLECULAR BIOLOGY
  • 1985-05. Molecular and general genetics of a hybrid foreign gene introduced into tobacco by direct gene transfer in MOLECULAR GENETICS AND GENOMICS
  • 1981-10. Mendelian transcmission of genes introduced into plants by the Ti plasmids of Agrobacterium tumefaciens in MOLECULAR GENETICS AND GENOMICS
  • 1991-02. Arabidopsis thaliana: protocol for plant regeneration from protoplasts in PLANT CELL REPORTS
  • 1988-12. High efficiency Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana leaf and cotyledon explants in PLANT CELL REPORTS
  • 1987-04. High meiotic stability of a foreign gene introduced into tobacco by Agrobacterium-mediated transformation in MOLECULAR GENETICS AND GENOMICS
  • 1991-02. Direct gene transfer to protoplasts of Arabidopsis thaliana in PLANT CELL REPORTS
  • 1986-12. Transposase activity of the Ac controlling element in maize is regulated by its degree of methylation in MOLECULAR GENETICS AND GENOMICS
  • 1984-04. The DNA of Arabidopsis thaliana in MOLECULAR GENETICS AND GENOMICS
  • 1984-11. Changes in T-DNA methylation and expression are associated with phenotypic variation and plant regeneration in a crown gall tumor line in MOLECULAR GENETICS AND GENOMICS
  • 1988-03. DNA methylation and tissue-specific transcription of the storage protein genes of maize in PLANT MOLECULAR BIOLOGY
  • Journal

    TITLE

    Molecular Genetics and Genomics

    ISSUE

    1-2

    VOLUME

    228

    Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00282454

    DOI

    http://dx.doi.org/10.1007/bf00282454

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1024311517

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/1886604


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Blotting, Northern", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Blotting, Southern", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Brassica", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cinnamates", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Crosses, Genetic", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "DNA, Recombinant", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Drug Resistance, Microbial", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Expression Regulation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Hygromycin B", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Methylation", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Institute for Plant Sciences, Federal Institute of Technology, Universit\u00e4tsstrasse 2, CH-8092, Z\u00fcrich, Switzerland", 
              "id": "http://www.grid.ac/institutes/grid.5801.c", 
              "name": [
                "Institute for Plant Sciences, Federal Institute of Technology, Universit\u00e4tsstrasse 2, CH-8092, Z\u00fcrich, Switzerland"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Scheid", 
            "givenName": "Ortrun Mittelsten", 
            "id": "sg:person.015035533521.12", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015035533521.12"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Institute for Plant Sciences, Federal Institute of Technology, Universit\u00e4tsstrasse 2, CH-8092, Z\u00fcrich, Switzerland", 
              "id": "http://www.grid.ac/institutes/grid.5801.c", 
              "name": [
                "Institute for Plant Sciences, Federal Institute of Technology, Universit\u00e4tsstrasse 2, CH-8092, Z\u00fcrich, Switzerland"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Paszkowski", 
            "givenName": "Jerzy", 
            "id": "sg:person.0737341345.45", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0737341345.45"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Institute for Plant Sciences, Federal Institute of Technology, Universit\u00e4tsstrasse 2, CH-8092, Z\u00fcrich, Switzerland", 
              "id": "http://www.grid.ac/institutes/grid.5801.c", 
              "name": [
                "Institute for Plant Sciences, Federal Institute of Technology, Universit\u00e4tsstrasse 2, CH-8092, Z\u00fcrich, Switzerland"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Potrykus", 
            "givenName": "Ingo", 
            "id": "sg:person.01206612000.14", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01206612000.14"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1007/bf00331506", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039629865", 
              "https://doi.org/10.1007/bf00331506"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00015672", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050274843", 
              "https://doi.org/10.1007/bf00015672"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00330255", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036019563", 
              "https://doi.org/10.1007/bf00330255"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00330935", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000803445", 
              "https://doi.org/10.1007/bf00330935"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00232335", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039842952", 
              "https://doi.org/10.1007/bf00232335"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00270619", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1045968668", 
              "https://doi.org/10.1007/bf00270619"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00383491", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036108700", 
              "https://doi.org/10.1007/bf00383491"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00232334", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035961470", 
              "https://doi.org/10.1007/bf00232334"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00272763", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015345244", 
              "https://doi.org/10.1007/bf00272763"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00330414", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038464131", 
              "https://doi.org/10.1007/bf00330414"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00338085", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032502861", 
              "https://doi.org/10.1007/bf00338085"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00305823", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053375676", 
              "https://doi.org/10.1007/bf00305823"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00633837", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040244648", 
              "https://doi.org/10.1007/bf00633837"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00752901", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025672137", 
              "https://doi.org/10.1007/bf00752901"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00329940", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037063138", 
              "https://doi.org/10.1007/bf00329940"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "1991-08", 
        "datePublishedReg": "1991-08-01", 
        "description": "Fifty percent of Arabidopsis thaliana plants transgenic for a hygromycin resistance gene failed to transmit the resistance phenotype to the progeny. The complete transgene was, however, inherited in all cases according to Mendelian laws as observed by Southern analysis. This discrepancy between genotype and phenotype was the result of a reduced level of transcript in the sensitive transformants. The gene inactivation occurred in plants with multicopy integration of the foreign DNA. No definite correlation was found between gene inactivity and methylation of cytidine residues in the transgene sequence. Explants from several sensitive transformed plants regained a low level of hygromycin resistance on callus induction medium. Subsequent generations obtained by self-pollination were sensitive. In contrast, spontaneous restoration of hygromycin tolerance was observed in seedlings originating from out-crosses with wild-type plants or a different sensitive transformant. A reduction of the copy number was not a prerequisite for spontaneous reactivation. The resistance was often lost again in the next generation. Inactivation and reactivation of the transgene are therefore reversible.", 
        "genre": "article", 
        "id": "sg:pub.10.1007/bf00282454", 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1297380", 
            "issn": [
              "1617-4615", 
              "1432-1874"
            ], 
            "name": "Molecular Genetics and Genomics", 
            "publisher": "Springer Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1-2", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "228"
          }
        ], 
        "keywords": [
          "wild-type plants", 
          "hygromycin resistance gene", 
          "levels of transcripts", 
          "callus induction medium", 
          "Arabidopsis thaliana", 
          "foreign DNA", 
          "gene inactivity", 
          "hygromycin resistance", 
          "Southern analysis", 
          "gene inactivation", 
          "transgene sequences", 
          "cytidine residues", 
          "multicopy integration", 
          "resistance genes", 
          "induction medium", 
          "copy number", 
          "Mendelian laws", 
          "plants", 
          "transgene", 
          "resistance phenotype", 
          "transformants", 
          "subsequent generations", 
          "inactivation", 
          "phenotype", 
          "Arabidopsis", 
          "thaliana", 
          "genes", 
          "methylation", 
          "seedlings", 
          "transcripts", 
          "progeny", 
          "reversible inactivation", 
          "DNA", 
          "residues", 
          "low levels", 
          "sequence", 
          "explants", 
          "genotypes", 
          "cross", 
          "tolerance", 
          "resistance", 
          "spontaneous restoration", 
          "next generation", 
          "spontaneous reactivation", 
          "reactivation", 
          "levels", 
          "generation", 
          "restoration", 
          "contrast", 
          "prerequisite", 
          "medium", 
          "analysis", 
          "definite correlation", 
          "number", 
          "results", 
          "reduction", 
          "correlation", 
          "integration", 
          "inactivity", 
          "discrepancy", 
          "percent", 
          "cases", 
          "law"
        ], 
        "name": "Reversible inactivation of a transgene in Arabidopsis thaliana", 
        "pagination": "104-112", 
        "productId": [
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1024311517"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/bf00282454"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "1886604"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/bf00282454", 
          "https://app.dimensions.ai/details/publication/pub.1024311517"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2022-08-04T16:52", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-springernature-scigraph/baseset/20220804/entities/gbq_results/article/article_261.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://doi.org/10.1007/bf00282454"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00282454'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00282454'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00282454'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00282454'


     

    This table displays all metadata directly associated to this object as RDF triples.

    238 TRIPLES      21 PREDICATES      114 URIs      91 LITERALS      17 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/bf00282454 schema:about N352fa510404a46bfafd317374d4cfda0
    2 N6889a5bd70374fcf81c95c9a31e289db
    3 N714daf3131af4942a25607e0957878a8
    4 N77bda80fb40e4e3e81bb51c8ef78dfa1
    5 N9b49784ad4d74c3195a4301242cb2e6d
    6 Na2d68bd4186f49e1b892da7de97f9dd5
    7 Nae9f48e61cf84fe1b9a895e3b2126e76
    8 Ndf45c482d88149618756dc709e0d4a4e
    9 Ne34afa6db0a54ba899a9bdc28448da0e
    10 Ne7f7a9625c9b4cc5b3a85ed1ae6520e2
    11 anzsrc-for:06
    12 anzsrc-for:0604
    13 schema:author N4e0f4bd60d2948f69f8461b0eda280a6
    14 schema:citation sg:pub.10.1007/bf00015672
    15 sg:pub.10.1007/bf00232334
    16 sg:pub.10.1007/bf00232335
    17 sg:pub.10.1007/bf00270619
    18 sg:pub.10.1007/bf00272763
    19 sg:pub.10.1007/bf00305823
    20 sg:pub.10.1007/bf00329940
    21 sg:pub.10.1007/bf00330255
    22 sg:pub.10.1007/bf00330414
    23 sg:pub.10.1007/bf00330935
    24 sg:pub.10.1007/bf00331506
    25 sg:pub.10.1007/bf00338085
    26 sg:pub.10.1007/bf00383491
    27 sg:pub.10.1007/bf00633837
    28 sg:pub.10.1007/bf00752901
    29 schema:datePublished 1991-08
    30 schema:datePublishedReg 1991-08-01
    31 schema:description Fifty percent of Arabidopsis thaliana plants transgenic for a hygromycin resistance gene failed to transmit the resistance phenotype to the progeny. The complete transgene was, however, inherited in all cases according to Mendelian laws as observed by Southern analysis. This discrepancy between genotype and phenotype was the result of a reduced level of transcript in the sensitive transformants. The gene inactivation occurred in plants with multicopy integration of the foreign DNA. No definite correlation was found between gene inactivity and methylation of cytidine residues in the transgene sequence. Explants from several sensitive transformed plants regained a low level of hygromycin resistance on callus induction medium. Subsequent generations obtained by self-pollination were sensitive. In contrast, spontaneous restoration of hygromycin tolerance was observed in seedlings originating from out-crosses with wild-type plants or a different sensitive transformant. A reduction of the copy number was not a prerequisite for spontaneous reactivation. The resistance was often lost again in the next generation. Inactivation and reactivation of the transgene are therefore reversible.
    32 schema:genre article
    33 schema:isAccessibleForFree false
    34 schema:isPartOf N69c48d9c2cf4443a9126a68c2a8f3651
    35 Nd48eb657971346f9beef0b34a226cb87
    36 sg:journal.1297380
    37 schema:keywords Arabidopsis
    38 Arabidopsis thaliana
    39 DNA
    40 Mendelian laws
    41 Southern analysis
    42 analysis
    43 callus induction medium
    44 cases
    45 contrast
    46 copy number
    47 correlation
    48 cross
    49 cytidine residues
    50 definite correlation
    51 discrepancy
    52 explants
    53 foreign DNA
    54 gene inactivation
    55 gene inactivity
    56 generation
    57 genes
    58 genotypes
    59 hygromycin resistance
    60 hygromycin resistance gene
    61 inactivation
    62 inactivity
    63 induction medium
    64 integration
    65 law
    66 levels
    67 levels of transcripts
    68 low levels
    69 medium
    70 methylation
    71 multicopy integration
    72 next generation
    73 number
    74 percent
    75 phenotype
    76 plants
    77 prerequisite
    78 progeny
    79 reactivation
    80 reduction
    81 residues
    82 resistance
    83 resistance genes
    84 resistance phenotype
    85 restoration
    86 results
    87 reversible inactivation
    88 seedlings
    89 sequence
    90 spontaneous reactivation
    91 spontaneous restoration
    92 subsequent generations
    93 thaliana
    94 tolerance
    95 transcripts
    96 transformants
    97 transgene
    98 transgene sequences
    99 wild-type plants
    100 schema:name Reversible inactivation of a transgene in Arabidopsis thaliana
    101 schema:pagination 104-112
    102 schema:productId Na264b78cd4e0491fbbe81bc9100a26b5
    103 Nb4aa0672dd424e2e817de42e3d513014
    104 Nd32169e21fe14649a9cfc9faf40f50dc
    105 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024311517
    106 https://doi.org/10.1007/bf00282454
    107 schema:sdDatePublished 2022-08-04T16:52
    108 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    109 schema:sdPublisher Nebfb7a7660834ea19fe9ec11220fcb10
    110 schema:url https://doi.org/10.1007/bf00282454
    111 sgo:license sg:explorer/license/
    112 sgo:sdDataset articles
    113 rdf:type schema:ScholarlyArticle
    114 N15b31607e86344db9d86cad3cf0bc25f rdf:first sg:person.01206612000.14
    115 rdf:rest rdf:nil
    116 N352fa510404a46bfafd317374d4cfda0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    117 schema:name Methylation
    118 rdf:type schema:DefinedTerm
    119 N4e0f4bd60d2948f69f8461b0eda280a6 rdf:first sg:person.015035533521.12
    120 rdf:rest Ndf16d86269db4625b5ee971773181de7
    121 N6889a5bd70374fcf81c95c9a31e289db schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    122 schema:name Gene Expression Regulation
    123 rdf:type schema:DefinedTerm
    124 N69c48d9c2cf4443a9126a68c2a8f3651 schema:issueNumber 1-2
    125 rdf:type schema:PublicationIssue
    126 N714daf3131af4942a25607e0957878a8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    127 schema:name Hygromycin B
    128 rdf:type schema:DefinedTerm
    129 N77bda80fb40e4e3e81bb51c8ef78dfa1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    130 schema:name Crosses, Genetic
    131 rdf:type schema:DefinedTerm
    132 N9b49784ad4d74c3195a4301242cb2e6d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    133 schema:name Blotting, Southern
    134 rdf:type schema:DefinedTerm
    135 Na264b78cd4e0491fbbe81bc9100a26b5 schema:name dimensions_id
    136 schema:value pub.1024311517
    137 rdf:type schema:PropertyValue
    138 Na2d68bd4186f49e1b892da7de97f9dd5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    139 schema:name Drug Resistance, Microbial
    140 rdf:type schema:DefinedTerm
    141 Nae9f48e61cf84fe1b9a895e3b2126e76 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    142 schema:name Cinnamates
    143 rdf:type schema:DefinedTerm
    144 Nb4aa0672dd424e2e817de42e3d513014 schema:name doi
    145 schema:value 10.1007/bf00282454
    146 rdf:type schema:PropertyValue
    147 Nd32169e21fe14649a9cfc9faf40f50dc schema:name pubmed_id
    148 schema:value 1886604
    149 rdf:type schema:PropertyValue
    150 Nd48eb657971346f9beef0b34a226cb87 schema:volumeNumber 228
    151 rdf:type schema:PublicationVolume
    152 Ndf16d86269db4625b5ee971773181de7 rdf:first sg:person.0737341345.45
    153 rdf:rest N15b31607e86344db9d86cad3cf0bc25f
    154 Ndf45c482d88149618756dc709e0d4a4e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    155 schema:name DNA, Recombinant
    156 rdf:type schema:DefinedTerm
    157 Ne34afa6db0a54ba899a9bdc28448da0e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    158 schema:name Brassica
    159 rdf:type schema:DefinedTerm
    160 Ne7f7a9625c9b4cc5b3a85ed1ae6520e2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    161 schema:name Blotting, Northern
    162 rdf:type schema:DefinedTerm
    163 Nebfb7a7660834ea19fe9ec11220fcb10 schema:name Springer Nature - SN SciGraph project
    164 rdf:type schema:Organization
    165 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    166 schema:name Biological Sciences
    167 rdf:type schema:DefinedTerm
    168 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    169 schema:name Genetics
    170 rdf:type schema:DefinedTerm
    171 sg:journal.1297380 schema:issn 1432-1874
    172 1617-4615
    173 schema:name Molecular Genetics and Genomics
    174 schema:publisher Springer Nature
    175 rdf:type schema:Periodical
    176 sg:person.01206612000.14 schema:affiliation grid-institutes:grid.5801.c
    177 schema:familyName Potrykus
    178 schema:givenName Ingo
    179 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01206612000.14
    180 rdf:type schema:Person
    181 sg:person.015035533521.12 schema:affiliation grid-institutes:grid.5801.c
    182 schema:familyName Scheid
    183 schema:givenName Ortrun Mittelsten
    184 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015035533521.12
    185 rdf:type schema:Person
    186 sg:person.0737341345.45 schema:affiliation grid-institutes:grid.5801.c
    187 schema:familyName Paszkowski
    188 schema:givenName Jerzy
    189 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0737341345.45
    190 rdf:type schema:Person
    191 sg:pub.10.1007/bf00015672 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050274843
    192 https://doi.org/10.1007/bf00015672
    193 rdf:type schema:CreativeWork
    194 sg:pub.10.1007/bf00232334 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035961470
    195 https://doi.org/10.1007/bf00232334
    196 rdf:type schema:CreativeWork
    197 sg:pub.10.1007/bf00232335 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039842952
    198 https://doi.org/10.1007/bf00232335
    199 rdf:type schema:CreativeWork
    200 sg:pub.10.1007/bf00270619 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045968668
    201 https://doi.org/10.1007/bf00270619
    202 rdf:type schema:CreativeWork
    203 sg:pub.10.1007/bf00272763 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015345244
    204 https://doi.org/10.1007/bf00272763
    205 rdf:type schema:CreativeWork
    206 sg:pub.10.1007/bf00305823 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053375676
    207 https://doi.org/10.1007/bf00305823
    208 rdf:type schema:CreativeWork
    209 sg:pub.10.1007/bf00329940 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037063138
    210 https://doi.org/10.1007/bf00329940
    211 rdf:type schema:CreativeWork
    212 sg:pub.10.1007/bf00330255 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036019563
    213 https://doi.org/10.1007/bf00330255
    214 rdf:type schema:CreativeWork
    215 sg:pub.10.1007/bf00330414 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038464131
    216 https://doi.org/10.1007/bf00330414
    217 rdf:type schema:CreativeWork
    218 sg:pub.10.1007/bf00330935 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000803445
    219 https://doi.org/10.1007/bf00330935
    220 rdf:type schema:CreativeWork
    221 sg:pub.10.1007/bf00331506 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039629865
    222 https://doi.org/10.1007/bf00331506
    223 rdf:type schema:CreativeWork
    224 sg:pub.10.1007/bf00338085 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032502861
    225 https://doi.org/10.1007/bf00338085
    226 rdf:type schema:CreativeWork
    227 sg:pub.10.1007/bf00383491 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036108700
    228 https://doi.org/10.1007/bf00383491
    229 rdf:type schema:CreativeWork
    230 sg:pub.10.1007/bf00633837 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040244648
    231 https://doi.org/10.1007/bf00633837
    232 rdf:type schema:CreativeWork
    233 sg:pub.10.1007/bf00752901 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025672137
    234 https://doi.org/10.1007/bf00752901
    235 rdf:type schema:CreativeWork
    236 grid-institutes:grid.5801.c schema:alternateName Institute for Plant Sciences, Federal Institute of Technology, Universitätsstrasse 2, CH-8092, Zürich, Switzerland
    237 schema:name Institute for Plant Sciences, Federal Institute of Technology, Universitätsstrasse 2, CH-8092, Zürich, Switzerland
    238 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...