An “instant gene bank” method for heterologous gene cloning: complementation of two Aspergillus nidulans mutants with Gaeumannomyces graminis DNA View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1994-02

AUTHORS

P. Bowyer, A. E. Osbourn, M. J. Daniels

ABSTRACT

We present a novel technique for gene cloning by complementation of mutations in Aspergillus nidulans with DNA from a heterologous organism, Gaeumannomyces graminis. This technique bypasses the time-consuming and difficult construction of gene libraries, making it both rapid and simple. The method relies on recombination between a fungal replicating vector pHELP1 and linear G. graminis genomic DNA during co-transformation. We were able to complement two out of seven A. nidulans mutants tested and to rescue transforming DNA from both in Escherichia coli. Complementation of the A. nidulans argB mutation resulted from integration of 8-10 kb segments of G. graminis DNA into pHELP1. The complementation of the A. nidulans pyrG mutation resulted from a complex rearrangement. Complementing DNA was shown to originate from G. graminis, and was capable of retransforming the original mutants to give the expected phenotype. More... »

PAGES

448-454

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00281795

DOI

http://dx.doi.org/10.1007/bf00281795

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1040241039

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/8121400


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Aspergillus nidulans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Base Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Blotting, Southern", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cloning, Molecular", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fungi", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Gene Library", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genes, Fungal", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genetic Complementation Test", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genetic Vectors", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mutation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Plant Diseases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Plasmids", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Restriction Mapping", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transformation, Genetic", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "name": [
            "The Sainsbury Laboratory, Norwich Research Park, NR4 7UH, Colney, Norwich, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bowyer", 
        "givenName": "P.", 
        "id": "sg:person.01106436040.10", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01106436040.10"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "The Sainsbury Laboratory, Norwich Research Park, NR4 7UH, Colney, Norwich, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Osbourn", 
        "givenName": "A. E.", 
        "id": "sg:person.0633370053.35", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0633370053.35"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "The Sainsbury Laboratory, Norwich Research Park, NR4 7UH, Colney, Norwich, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Daniels", 
        "givenName": "M. J.", 
        "id": "sg:person.01270777640.75", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01270777640.75"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/bf00331649", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000287731", 
          "https://doi.org/10.1007/bf00331649"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00331649", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000287731", 
          "https://doi.org/10.1007/bf00331649"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1472-765x.1985.tb01479.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004082457"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.4.4.651", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006085503"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.83.15.5602", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013572162"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(79)90093-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016794939"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/287869a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018289259", 
          "https://doi.org/10.1038/287869a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00281798", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021963601", 
          "https://doi.org/10.1007/bf00281798"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00281798", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021963601", 
          "https://doi.org/10.1007/bf00281798"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0006-291x(83)91828-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023237840"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-1-4899-1710-2_26", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027900082", 
          "https://doi.org/10.1007/978-1-4899-1710-2_26"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0147-5975(90)90082-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028897629"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00331067", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028982770", 
          "https://doi.org/10.1007/bf00331067"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00331067", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028982770", 
          "https://doi.org/10.1007/bf00331067"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0065-2660(08)60408-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036702189"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0885-5765(05)80121-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039985978"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.5.1.59", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040667664"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/7.6.1513", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046335196"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00351716", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047959874", 
          "https://doi.org/10.1007/bf00351716"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00351716", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047959874", 
          "https://doi.org/10.1007/bf00351716"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(91)90104-j", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048600077"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(91)90104-j", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048600077"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(87)90201-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051219725"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(87)90201-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051219725"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1139/g88-084", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1079487336"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1985.tb03777.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1080017375"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4148/1941-4765.1533", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1084453630"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1994-02", 
    "datePublishedReg": "1994-02-01", 
    "description": "We present a novel technique for gene cloning by complementation of mutations in Aspergillus nidulans with DNA from a heterologous organism, Gaeumannomyces graminis. This technique bypasses the time-consuming and difficult construction of gene libraries, making it both rapid and simple. The method relies on recombination between a fungal replicating vector pHELP1 and linear G. graminis genomic DNA during co-transformation. We were able to complement two out of seven A. nidulans mutants tested and to rescue transforming DNA from both in Escherichia coli. Complementation of the A. nidulans argB mutation resulted from integration of 8-10 kb segments of G. graminis DNA into pHELP1. The complementation of the A. nidulans pyrG mutation resulted from a complex rearrangement. Complementing DNA was shown to originate from G. graminis, and was capable of retransforming the original mutants to give the expected phenotype.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/bf00281795", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1297380", 
        "issn": [
          "1617-4615", 
          "1432-1874"
        ], 
        "name": "Molecular Genetics and Genomics", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "4", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "242"
      }
    ], 
    "name": "An \u201cinstant gene bank\u201d method for heterologous gene cloning: complementation of two Aspergillus nidulans mutants with Gaeumannomyces graminis DNA", 
    "pagination": "448-454", 
    "productId": [
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/bf00281795"
        ]
      }, 
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "bfb1810df3f9afa8ceeaa127015518e16517cc055628bc7bab535e822db94ddf"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1040241039"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0125036"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "8121400"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/bf00281795", 
      "https://app.dimensions.ai/details/publication/pub.1040241039"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-15T08:51", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000374_0000000374/records_119737_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007/BF00281795"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00281795'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00281795'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00281795'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00281795'


 

This table displays all metadata directly associated to this object as RDF triples.

215 TRIPLES      21 PREDICATES      65 URIs      36 LITERALS      24 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/bf00281795 schema:about N033f488d3ff448d782620885f258cb94
2 N07d95fb967874058bf2af31631e820a2
3 N1630ce81a3b14ab184de53d43d25b450
4 N34c784fd73fd42999bd43d76fff2287d
5 N51f36df7ddec472c9bd42a57c4b80d09
6 N68398e50a9be430e82474601d2e4a1e5
7 N79dbd56ebe9a498b9ca962447ef0cb47
8 N8177a123a46c41de8a5732972c872f77
9 N86ba93582f354c82be3cbe0e191b12a9
10 N86c1fdf9b89a46719b4ab5e5f3bc5f76
11 N976ce39d0d8b4c059bc7543f8c744540
12 N99700d514a3d47858267a0f1e5a1acc7
13 Nb1a5880605ad46e1afd4595fd6410840
14 Nbe69ac112aad477185da9c27cc7aafcc
15 Nc5437ed8a16d4db88c77c705d6093035
16 anzsrc-for:06
17 anzsrc-for:0604
18 schema:author Nd284bbcbfd7c41eabcc4d065456c0788
19 schema:citation sg:pub.10.1007/978-1-4899-1710-2_26
20 sg:pub.10.1007/bf00281798
21 sg:pub.10.1007/bf00331067
22 sg:pub.10.1007/bf00331649
23 sg:pub.10.1007/bf00351716
24 sg:pub.10.1038/287869a0
25 https://doi.org/10.1002/j.1460-2075.1985.tb03777.x
26 https://doi.org/10.1016/0006-291x(83)91828-4
27 https://doi.org/10.1016/0092-8674(79)90093-x
28 https://doi.org/10.1016/0147-5975(90)90082-5
29 https://doi.org/10.1016/0378-1119(87)90201-0
30 https://doi.org/10.1016/0378-1119(91)90104-j
31 https://doi.org/10.1016/s0065-2660(08)60408-3
32 https://doi.org/10.1016/s0885-5765(05)80121-3
33 https://doi.org/10.1073/pnas.83.15.5602
34 https://doi.org/10.1093/nar/7.6.1513
35 https://doi.org/10.1111/j.1472-765x.1985.tb01479.x
36 https://doi.org/10.1128/mcb.4.4.651
37 https://doi.org/10.1128/mcb.5.1.59
38 https://doi.org/10.1139/g88-084
39 https://doi.org/10.4148/1941-4765.1533
40 schema:datePublished 1994-02
41 schema:datePublishedReg 1994-02-01
42 schema:description We present a novel technique for gene cloning by complementation of mutations in Aspergillus nidulans with DNA from a heterologous organism, Gaeumannomyces graminis. This technique bypasses the time-consuming and difficult construction of gene libraries, making it both rapid and simple. The method relies on recombination between a fungal replicating vector pHELP1 and linear G. graminis genomic DNA during co-transformation. We were able to complement two out of seven A. nidulans mutants tested and to rescue transforming DNA from both in Escherichia coli. Complementation of the A. nidulans argB mutation resulted from integration of 8-10 kb segments of G. graminis DNA into pHELP1. The complementation of the A. nidulans pyrG mutation resulted from a complex rearrangement. Complementing DNA was shown to originate from G. graminis, and was capable of retransforming the original mutants to give the expected phenotype.
43 schema:genre research_article
44 schema:inLanguage en
45 schema:isAccessibleForFree false
46 schema:isPartOf N1567443662ea4f76a8b767cf5f07d71f
47 Nd8997438499347d08b1c2740370b6587
48 sg:journal.1297380
49 schema:name An “instant gene bank” method for heterologous gene cloning: complementation of two Aspergillus nidulans mutants with Gaeumannomyces graminis DNA
50 schema:pagination 448-454
51 schema:productId N6ff03d0024de4be585c64c7eaf642c79
52 N7da8af10cd5047d09e4119cfc610866a
53 Nd4dda825c95145b9981377be23f51fe3
54 Ne04cb8677d164885862cd90d50809831
55 Nfe99ebe7d7cb4defafa24893e2ac9a38
56 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040241039
57 https://doi.org/10.1007/bf00281795
58 schema:sdDatePublished 2019-04-15T08:51
59 schema:sdLicense https://scigraph.springernature.com/explorer/license/
60 schema:sdPublisher N90eb027de73245aa8e188cc7f283ec1e
61 schema:url http://link.springer.com/10.1007/BF00281795
62 sgo:license sg:explorer/license/
63 sgo:sdDataset articles
64 rdf:type schema:ScholarlyArticle
65 N033f488d3ff448d782620885f258cb94 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
66 schema:name Plasmids
67 rdf:type schema:DefinedTerm
68 N07d95fb967874058bf2af31631e820a2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
69 schema:name Aspergillus nidulans
70 rdf:type schema:DefinedTerm
71 N0a89ba7060294e0eaa16f13328ecf31d schema:name The Sainsbury Laboratory, Norwich Research Park, NR4 7UH, Colney, Norwich, UK
72 rdf:type schema:Organization
73 N1567443662ea4f76a8b767cf5f07d71f schema:volumeNumber 242
74 rdf:type schema:PublicationVolume
75 N1630ce81a3b14ab184de53d43d25b450 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
76 schema:name Blotting, Southern
77 rdf:type schema:DefinedTerm
78 N16574499a3474e9fb02569a3fede74ac schema:name The Sainsbury Laboratory, Norwich Research Park, NR4 7UH, Colney, Norwich, UK
79 rdf:type schema:Organization
80 N34c784fd73fd42999bd43d76fff2287d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
81 schema:name Mutation
82 rdf:type schema:DefinedTerm
83 N51f36df7ddec472c9bd42a57c4b80d09 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
84 schema:name Cloning, Molecular
85 rdf:type schema:DefinedTerm
86 N6133695ffb71458e82ba5b0029e80fb7 rdf:first sg:person.0633370053.35
87 rdf:rest Nf5efc938a55847e294c5b78b49bbcf70
88 N68398e50a9be430e82474601d2e4a1e5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
89 schema:name Fungi
90 rdf:type schema:DefinedTerm
91 N6f1258368a724a89a3536a44215307dc schema:name The Sainsbury Laboratory, Norwich Research Park, NR4 7UH, Colney, Norwich, UK
92 rdf:type schema:Organization
93 N6ff03d0024de4be585c64c7eaf642c79 schema:name dimensions_id
94 schema:value pub.1040241039
95 rdf:type schema:PropertyValue
96 N79dbd56ebe9a498b9ca962447ef0cb47 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
97 schema:name Genes, Fungal
98 rdf:type schema:DefinedTerm
99 N7da8af10cd5047d09e4119cfc610866a schema:name doi
100 schema:value 10.1007/bf00281795
101 rdf:type schema:PropertyValue
102 N8177a123a46c41de8a5732972c872f77 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
103 schema:name Genetic Vectors
104 rdf:type schema:DefinedTerm
105 N86ba93582f354c82be3cbe0e191b12a9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
106 schema:name Plant Diseases
107 rdf:type schema:DefinedTerm
108 N86c1fdf9b89a46719b4ab5e5f3bc5f76 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
109 schema:name Transformation, Genetic
110 rdf:type schema:DefinedTerm
111 N90eb027de73245aa8e188cc7f283ec1e schema:name Springer Nature - SN SciGraph project
112 rdf:type schema:Organization
113 N976ce39d0d8b4c059bc7543f8c744540 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
114 schema:name Base Sequence
115 rdf:type schema:DefinedTerm
116 N99700d514a3d47858267a0f1e5a1acc7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
117 schema:name Restriction Mapping
118 rdf:type schema:DefinedTerm
119 Nb1a5880605ad46e1afd4595fd6410840 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
120 schema:name Genetic Complementation Test
121 rdf:type schema:DefinedTerm
122 Nbe69ac112aad477185da9c27cc7aafcc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
123 schema:name Gene Library
124 rdf:type schema:DefinedTerm
125 Nc5437ed8a16d4db88c77c705d6093035 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
126 schema:name Molecular Sequence Data
127 rdf:type schema:DefinedTerm
128 Nd284bbcbfd7c41eabcc4d065456c0788 rdf:first sg:person.01106436040.10
129 rdf:rest N6133695ffb71458e82ba5b0029e80fb7
130 Nd4dda825c95145b9981377be23f51fe3 schema:name readcube_id
131 schema:value bfb1810df3f9afa8ceeaa127015518e16517cc055628bc7bab535e822db94ddf
132 rdf:type schema:PropertyValue
133 Nd8997438499347d08b1c2740370b6587 schema:issueNumber 4
134 rdf:type schema:PublicationIssue
135 Ne04cb8677d164885862cd90d50809831 schema:name pubmed_id
136 schema:value 8121400
137 rdf:type schema:PropertyValue
138 Nf5efc938a55847e294c5b78b49bbcf70 rdf:first sg:person.01270777640.75
139 rdf:rest rdf:nil
140 Nfe99ebe7d7cb4defafa24893e2ac9a38 schema:name nlm_unique_id
141 schema:value 0125036
142 rdf:type schema:PropertyValue
143 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
144 schema:name Biological Sciences
145 rdf:type schema:DefinedTerm
146 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
147 schema:name Genetics
148 rdf:type schema:DefinedTerm
149 sg:journal.1297380 schema:issn 1432-1874
150 1617-4615
151 schema:name Molecular Genetics and Genomics
152 rdf:type schema:Periodical
153 sg:person.01106436040.10 schema:affiliation N0a89ba7060294e0eaa16f13328ecf31d
154 schema:familyName Bowyer
155 schema:givenName P.
156 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01106436040.10
157 rdf:type schema:Person
158 sg:person.01270777640.75 schema:affiliation N16574499a3474e9fb02569a3fede74ac
159 schema:familyName Daniels
160 schema:givenName M. J.
161 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01270777640.75
162 rdf:type schema:Person
163 sg:person.0633370053.35 schema:affiliation N6f1258368a724a89a3536a44215307dc
164 schema:familyName Osbourn
165 schema:givenName A. E.
166 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0633370053.35
167 rdf:type schema:Person
168 sg:pub.10.1007/978-1-4899-1710-2_26 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027900082
169 https://doi.org/10.1007/978-1-4899-1710-2_26
170 rdf:type schema:CreativeWork
171 sg:pub.10.1007/bf00281798 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021963601
172 https://doi.org/10.1007/bf00281798
173 rdf:type schema:CreativeWork
174 sg:pub.10.1007/bf00331067 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028982770
175 https://doi.org/10.1007/bf00331067
176 rdf:type schema:CreativeWork
177 sg:pub.10.1007/bf00331649 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000287731
178 https://doi.org/10.1007/bf00331649
179 rdf:type schema:CreativeWork
180 sg:pub.10.1007/bf00351716 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047959874
181 https://doi.org/10.1007/bf00351716
182 rdf:type schema:CreativeWork
183 sg:pub.10.1038/287869a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018289259
184 https://doi.org/10.1038/287869a0
185 rdf:type schema:CreativeWork
186 https://doi.org/10.1002/j.1460-2075.1985.tb03777.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1080017375
187 rdf:type schema:CreativeWork
188 https://doi.org/10.1016/0006-291x(83)91828-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023237840
189 rdf:type schema:CreativeWork
190 https://doi.org/10.1016/0092-8674(79)90093-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1016794939
191 rdf:type schema:CreativeWork
192 https://doi.org/10.1016/0147-5975(90)90082-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028897629
193 rdf:type schema:CreativeWork
194 https://doi.org/10.1016/0378-1119(87)90201-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051219725
195 rdf:type schema:CreativeWork
196 https://doi.org/10.1016/0378-1119(91)90104-j schema:sameAs https://app.dimensions.ai/details/publication/pub.1048600077
197 rdf:type schema:CreativeWork
198 https://doi.org/10.1016/s0065-2660(08)60408-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036702189
199 rdf:type schema:CreativeWork
200 https://doi.org/10.1016/s0885-5765(05)80121-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039985978
201 rdf:type schema:CreativeWork
202 https://doi.org/10.1073/pnas.83.15.5602 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013572162
203 rdf:type schema:CreativeWork
204 https://doi.org/10.1093/nar/7.6.1513 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046335196
205 rdf:type schema:CreativeWork
206 https://doi.org/10.1111/j.1472-765x.1985.tb01479.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1004082457
207 rdf:type schema:CreativeWork
208 https://doi.org/10.1128/mcb.4.4.651 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006085503
209 rdf:type schema:CreativeWork
210 https://doi.org/10.1128/mcb.5.1.59 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040667664
211 rdf:type schema:CreativeWork
212 https://doi.org/10.1139/g88-084 schema:sameAs https://app.dimensions.ai/details/publication/pub.1079487336
213 rdf:type schema:CreativeWork
214 https://doi.org/10.4148/1941-4765.1533 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084453630
215 rdf:type schema:CreativeWork
 




Preview window. Press ESC to close (or click here)


...