Chromosome walking with YAC clones in Arabidopsis: isolation of 1700 kb of contiguous DNA on chromosome 5, including a 300 ... View Full Text


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Article Info

DATE

1993-05

AUTHORS

Joanna Putterill, Frances Robson, Karen Lee, George Coupland

ABSTRACT

The co mutation of Arabidopsis thaliana causes a late-flowering phenotype that is insensitive to day-ength. The mutation was mapped previously to the upper arm of chromosome 5, approximately 1.6 cM from the chalcone synthase gene (CHS). We were provided with five yeast artificial chromosome (YAC) libraries and used these to perform a chromosome walk from CHS to the CO gene. In this paper we report the isolation of 1700 kb of contiguous Arabidopsis DNA, which represents approximately 1%–2% of the genome, inserted in YACs. This required the detailed analysis of 67 YACs, from which 87 end probes were isolated and examined in hybridisation experiments. This analysis showed that approximately 40% of the YACs presented problems in chromosome walking experiments because they contained repetitive sequence at one of their termini, were chimaeric or because part of the plant DNA was deleted. DNA fragments isolated from YACs were used as restriction fragment length polymorphism (RFLP) markers to localize CO to a 300 kb region within the cloned DNA. We compare the physical distance between CHS and CO with the genetic distance and find that in this region 1 cM is equivalent to approximately 200 kb. More... »

PAGES

145-157

References to SciGraph publications

  • 1991-05. Construction and characterization of a yeast artificial chromosome library of Arabidopsis which is suitable for chromosome walking in MOLECULAR GENETICS AND GENOMICS
  • 1991-08. Nuclear DNA content of some important plant species in PLANT MOLECULAR BIOLOGY REPORTER
  • 1989-02. Pulsed field gel electrophoresis and physical mapping of large DNA fragments in the Tm-2a region of chromosome 9 in tomato in MOLECULAR GENETICS AND GENOMICS
  • 1992-01. Correlation of genetic and physical structure in the region surrounding the I2Fusarium oxysporum resistance locus in tomato in MOLECULAR GENETICS AND GENOMICS
  • 1990-04. Isolation of single-copy-sequence clones from a yeast artificial chromosome library of randomly-sheared Arabidopsis thaliana DNA in PLANT MOLECULAR BIOLOGY
  • 1990-11. A rapid and inexpensive method for isolation of total DNA from dehydrated plant tissue in PLANT MOLECULAR BIOLOGY REPORTER
  • 1984-04. The DNA of Arabidopsis thaliana in MOLECULAR GENETICS AND GENOMICS
  • 1990-07. The protein encoded by the Arabidopsis homeotic gene agamous resembles transcription factors in NATURE
  • 1992-05. The complete DNA sequence of yeast chromosome III in NATURE
  • 1991-02. A recombination hotspot in the maize A1 intragenic region in THEORETICAL AND APPLIED GENETICS
  • 1991-10. A DNA Transformation–Competent Arabidopsis Genomic Library in Agrobacterium in BIO/TECHNOLOGY
  • 1991-09. A genetic and physiological analysis of late flowering mutants in Arabidopsis thaliana in MOLECULAR GENETICS AND GENOMICS
  • 1988-09. Genome linking with yeast artificial chromosomes in NATURE
  • 1991-07. Mapping genes essential for embryo development in Arabidopsis thaliana in MOLECULAR GENETICS AND GENOMICS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00281613

    DOI

    http://dx.doi.org/10.1007/bf00281613

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1082650860

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/8099710


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