Fluorescence cytophotometry: a valuable method for the quantitative determination of nuclear feulgen-DNA View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1968-06

AUTHORS

Norbert Böhm, Ernst Sprenger

ABSTRACT

A cytofluorometric apparatus with incident illumination for fluorescence excitation is described here. Cytophotometric fluorescence measurements (UV- and blue excitation) of acriflavine-acridine yellow-, coriphosphine- and pararosaniline-Schiff stained di-, tetra- and octoploid liver nuclei, leucocytes and sperms (Feulgen reaction) were found to agree with the absorbance data obtained from the same slide by means of the integrating microdensitometer. The stoichiometry of the fluorescence emission is discussed in detail. It is emphasized that not a linear, but an exponential relationship exists between the emitted fluorescence intensity and the concentration of the fluorescent substance. Cytofluorometry of Feulgen-stained nuclei has proved to be as reliable and fast as the absorbance scanning measurements at the intergrating microdensitometer. More... »

PAGES

100-118

References to SciGraph publications

  • 1968-09. Proportionalitätsfehler bei der Feulgen-Hydrolyse in HISTOCHEMISTRY AND CELL BIOLOGY
  • 1924-12. Über einen weitverbreiteten festen Aldehyd in PFLÜGERS ARCHIV - EUROPEAN JOURNAL OF PHYSIOLOGY
  • 1959-11. Schiff-type reagents in cytochemistry in HISTOCHEMISTRY AND CELL BIOLOGY
  • 1962-01. The metachromasia of fluorescence of acridine dyes in HISTOCHEMISTRY AND CELL BIOLOGY
  • 1962-03. Studies on DNA in leucocytes and related cells of mammals in HISTOCHEMISTRY AND CELL BIOLOGY
  • 1968-06. Einfluß der Fixierung und der Säurekonzentration auf die Feulgen-Hydrolyse bei 28° C in HISTOCHEMISTRY AND CELL BIOLOGY
  • 1959-12. Fluorescent Schiff-Type Reagents for Cytochemical Detection of Polyaldehyde Moieties in Sections and Smears in NATURE
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00280607

    DOI

    http://dx.doi.org/10.1007/bf00280607

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1081317973

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/5751084


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