Ontology type: schema:ScholarlyArticle
1990-03
AUTHORSLean Han San, Fernand Vedel, Darasinh Sihachakr, René Rémy
ABSTRACTMesophyl protoplasts of two genotypes of cultivated tomato (Lycopersicon esculentum Mill.) and one of its wild relative species (Lycopersicon peruvianum Mill.) were fused by using electrofusion and polyethyleneglycol-induced fusion. Forty-three fertile tetraploid somatic hybrid plants, each deriving from separate calli, were recovered from both fusion procedures. Electrofusion appeared more efficient than chemical fusion for the production of somatic hybrids. These plants appeared morphologically similar, whatever the fusion procedure and tomato genotype. They had intermediate leaf, inflorescence, and flower morphology. After self-pollination, the hybrids set fruit of intermediate size and color. The hybrid nature of these plants was confirmed by isoelectric focusing of the Rubisco small subunits used as nuclear markers. L. esculentum and L. peruvianum were distinguished by means of two chloroplast markers: CF1-ATPase β subunit as analyzed by isoelectro-focusing and ct DNA restriction patterns. All hybrids displayed both ct markers of only one parent with no biased transmission. Mitochondrial (mt) DNAs were prepared from flower buds by using miniaturized CsCl gradients. Preliminary analysis indicated that mt genomes from the hybrids all differed from those of both parents. mt DNA Sall restriction enzyme analysis revealed that all but two hybrids contained one novel fragment of 13.5 kb. Gene mapping experiments showed that the mt apocytochrome b and ATPase subunit 9 homologies in the somatic hybrid mt DNA resembled L. esculentum and L. peruvianum, respectively; the mt nad5 probe distinguished at least four distinct patterns in the hybrids. These results indicated that mt DNA rearrangements involving intergenomic recombinations occurred through protoplast fusion. A greater mt DNA polymorphism was induced with chemical fusion than with electrofusion. More... »
PAGES17-26
http://scigraph.springernature.com/pub.10.1007/bf00280362
DOIhttp://dx.doi.org/10.1007/bf00280362
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1023882389
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Genetics",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "University of Paris-Sud",
"id": "https://www.grid.ac/institutes/grid.5842.b",
"name": [
"Laboratoires d'Am\u00e9lioration des Plantes, Universit\u00e9 de Paris Sud, C.N.R.S. URA 115, B\u00e2t. 360, F-91405, Orsay Cedex, France"
],
"type": "Organization"
},
"familyName": "San",
"givenName": "Lean Han",
"id": "sg:person.016364263447.52",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016364263447.52"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Paris-Sud",
"id": "https://www.grid.ac/institutes/grid.5842.b",
"name": [
"G\u00e9n\u00e9tique Mol\u00e9culaire des Plantes, Universit\u00e9 de Paris Sud, C.N.R.S. URA 115, B\u00e2t. 360, F-91405, Orsay Cedex, France"
],
"type": "Organization"
},
"familyName": "Vedel",
"givenName": "Fernand",
"id": "sg:person.01230546500.45",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01230546500.45"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Paris-Sud",
"id": "https://www.grid.ac/institutes/grid.5842.b",
"name": [
"Morphog\u00e9n\u00e8se V\u00e9g\u00e9tale Exp\u00e9rimentale, Universit\u00e9 de Paris Sud, C.N.R.S. URA 115, B\u00e2t. 360, F-91405, Orsay Cedex, France"
],
"type": "Organization"
},
"familyName": "Sihachakr",
"givenName": "Darasinh",
"id": "sg:person.01154762366.71",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01154762366.71"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Paris-Sud",
"id": "https://www.grid.ac/institutes/grid.5842.b",
"name": [
"G\u00e9n\u00e9tique Mol\u00e9culaire des Plantes, Universit\u00e9 de Paris Sud, C.N.R.S. URA 115, B\u00e2t. 360, F-91405, Orsay Cedex, France"
],
"type": "Organization"
},
"familyName": "R\u00e9my",
"givenName": "Ren\u00e9",
"id": "sg:person.01064467004.61",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01064467004.61"
],
"type": "Person"
}
],
"citation": [
{
"id": "https://doi.org/10.1016/0012-1606(73)90169-3",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1002894965"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00263265",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1003180120",
"https://doi.org/10.1007/bf00263265"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00263265",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1003180120",
"https://doi.org/10.1007/bf00263265"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00331077",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1003226588",
"https://doi.org/10.1007/bf00331077"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00334821",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1004307926",
"https://doi.org/10.1007/bf00334821"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1073/pnas.82.4.1015",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1005008331"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00268762",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1005203430",
"https://doi.org/10.1007/bf00268762"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00268762",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1005203430",
"https://doi.org/10.1007/bf00268762"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0176-1617(86)80145-6",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1007116425"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00389421",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1007373293",
"https://doi.org/10.1007/bf00389421"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00389421",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1007373293",
"https://doi.org/10.1007/bf00389421"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/281401a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1009194683",
"https://doi.org/10.1038/281401a0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/281401a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1009194683",
"https://doi.org/10.1038/281401a0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/227680a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1010419937",
"https://doi.org/10.1038/227680a0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00249146",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1010469702",
"https://doi.org/10.1007/bf00249146"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00249146",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1010469702",
"https://doi.org/10.1007/bf00249146"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf02906548",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1012846344",
"https://doi.org/10.1007/bf02906548"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf02906548",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1012846344",
"https://doi.org/10.1007/bf02906548"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00569342",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1018429991",
"https://doi.org/10.1007/bf00569342"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1111/j.1399-3054.1978.tb01565.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1020907619"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00276566",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1021437496",
"https://doi.org/10.1007/bf00276566"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00276566",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1021437496",
"https://doi.org/10.1007/bf00276566"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1111/j.1399-3054.1962.tb08052.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1023703429"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00332521",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1024520341",
"https://doi.org/10.1007/bf00332521"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/307437a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1025034215",
"https://doi.org/10.1038/307437a0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00261455",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1025662800",
"https://doi.org/10.1007/bf00261455"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00261455",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1025662800",
"https://doi.org/10.1007/bf00261455"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00334683",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1026086025",
"https://doi.org/10.1007/bf00334683"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00381175",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1026313797",
"https://doi.org/10.1007/bf00381175"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00381175",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1026313797",
"https://doi.org/10.1007/bf00381175"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00017450",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1026698689",
"https://doi.org/10.1007/bf00017450"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00017450",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1026698689",
"https://doi.org/10.1007/bf00017450"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1104/pp.24.1.1",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1029730230"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1073/pnas.74.11.5109",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1032655405"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0168-9452(88)90127-6",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1033384298"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00322444",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1034429788",
"https://doi.org/10.1007/bf00322444"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00322444",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1034429788",
"https://doi.org/10.1007/bf00322444"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/263440a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1035201014",
"https://doi.org/10.1038/263440a0"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/b978-0-12-715003-1.50036-8",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1035824098"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00422911",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1035959476",
"https://doi.org/10.1007/bf00422911"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1073/pnas.82.20.6960",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1041268799"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00337947",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1042928478",
"https://doi.org/10.1007/bf00337947"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0074-7696(08)60324-1",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1048933287"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1146/annurev.pp.38.060187.002135",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1050749233"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00570903",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1051438839",
"https://doi.org/10.1007/bf00570903"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00274711",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1052908617",
"https://doi.org/10.1007/bf00274711"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00274711",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1052908617",
"https://doi.org/10.1007/bf00274711"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1126/science.219.4585.683",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1062526111"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/j.1460-2075.1984.tb02098.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1077183697"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/j.1537-2197.1951.tb14804.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1100751520"
],
"type": "CreativeWork"
}
],
"datePublished": "1990-03",
"datePublishedReg": "1990-03-01",
"description": "Mesophyl protoplasts of two genotypes of cultivated tomato (Lycopersicon esculentum Mill.) and one of its wild relative species (Lycopersicon peruvianum Mill.) were fused by using electrofusion and polyethyleneglycol-induced fusion. Forty-three fertile tetraploid somatic hybrid plants, each deriving from separate calli, were recovered from both fusion procedures. Electrofusion appeared more efficient than chemical fusion for the production of somatic hybrids. These plants appeared morphologically similar, whatever the fusion procedure and tomato genotype. They had intermediate leaf, inflorescence, and flower morphology. After self-pollination, the hybrids set fruit of intermediate size and color. The hybrid nature of these plants was confirmed by isoelectric focusing of the Rubisco small subunits used as nuclear markers. L. esculentum and L. peruvianum were distinguished by means of two chloroplast markers: CF1-ATPase \u03b2 subunit as analyzed by isoelectro-focusing and ct DNA restriction patterns. All hybrids displayed both ct markers of only one parent with no biased transmission. Mitochondrial (mt) DNAs were prepared from flower buds by using miniaturized CsCl gradients. Preliminary analysis indicated that mt genomes from the hybrids all differed from those of both parents. mt DNA Sall restriction enzyme analysis revealed that all but two hybrids contained one novel fragment of 13.5 kb. Gene mapping experiments showed that the mt apocytochrome b and ATPase subunit 9 homologies in the somatic hybrid mt DNA resembled L. esculentum and L. peruvianum, respectively; the mt nad5 probe distinguished at least four distinct patterns in the hybrids. These results indicated that mt DNA rearrangements involving intergenomic recombinations occurred through protoplast fusion. A greater mt DNA polymorphism was induced with chemical fusion than with electrofusion.",
"genre": "research_article",
"id": "sg:pub.10.1007/bf00280362",
"inLanguage": [
"en"
],
"isAccessibleForFree": false,
"isPartOf": [
{
"id": "sg:journal.1297380",
"issn": [
"1617-4615",
"1432-1874"
],
"name": "Molecular Genetics and Genomics",
"type": "Periodical"
},
{
"issueNumber": "1",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "221"
}
],
"name": "Morphological and molecular characterization of fertile tetraploid somatic hybrids produced by protoplast electrofusion and PEG-induced fusion between Lycopersicon esculentum Mill. and Lycopersicon peruvianum Mill.",
"pagination": "17-26",
"productId": [
{
"name": "readcube_id",
"type": "PropertyValue",
"value": [
"71c75272e215c22ce665f304d75993f5dcfcd97ecc14c0c6f292ff62468dca56"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1007/bf00280362"
]
},
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1023882389"
]
}
],
"sameAs": [
"https://doi.org/10.1007/bf00280362",
"https://app.dimensions.ai/details/publication/pub.1023882389"
],
"sdDataset": "articles",
"sdDatePublished": "2019-04-11T13:47",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000371_0000000371/records_130792_00000002.jsonl",
"type": "ScholarlyArticle",
"url": "http://link.springer.com/10.1007/BF00280362"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00280362'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00280362'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00280362'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00280362'
This table displays all metadata directly associated to this object as RDF triples.
221 TRIPLES
21 PREDICATES
65 URIs
19 LITERALS
7 BLANK NODES