Characterization of the Staphylococcus aureus chromosomal gene pcrA, identified by mutations affecting plasmid pT181 replication View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1993-10

AUTHORS

Serban Iordanescu

ABSTRACT

The Staphylococcus aureus chromosomal gene pcrA, identified by mutations, such as pcrA3, that affect plasmid pT181 replication, has been cloned and sequenced. The pcrA gene encodes a protein with significant similarity (40% identity) to two Escherichia coli helicases: the helicase II encoded by the uvrD gene and the Rep helicase. The pcrA3 mutation was found to be a C to T transition leading to a threonine to isoleucine substitution at amino acid residue 61 of the protein. The pcrA gene seems to belong to an operon containing at least one other gene, tentatively named pcrB, upstream from pcrA. The PcrA protein was shown to be essential for cell viability and overproduction has deleterious effects on the host and plasmid replication. More... »

PAGES

185-192

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00280216

DOI

http://dx.doi.org/10.1007/bf00280216

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1040761738

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/8232203


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