Somatic hybrid plants obtained by protoplast fusion between Citrus sinensis and Poncirus trifoliata View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1985-11

AUTHORS

T. Ohgawara, S. Kobayashi, E. Ohgawara, H. Uchimiya, S. Ishii

ABSTRACT

Somatic hybrid plants of Rutaceae were obtained by protoplast fusion between Citrus sinensis Osb. ('Trovita' orange) and Poncirus trifoliata. Protoplasts isolated from embryogenic cells of C. sinensis and from leaves of P. trifoliata, and the culture of fusion products in the presence of high concentrations of sucrose were essential requirements for the selection of hybrids. Green globular embryoids derived from protoplasts resulted in the regeneration of trifoliate plants. Other morphological characters of these plants were intermediate between both parents. The chromosome number in one of the hybrid plants was 36, which was the sum of C. sinensis (2n=18) and P. trifoliata (2n=18). EcoRI restriction analysis of rDNA confirmed the presence of parental nuclear DNAs in the hybrid. More... »

PAGES

1-4

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00278245

DOI

http://dx.doi.org/10.1007/bf00278245

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1004941921

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24247330


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