Analysis of organelle genomes in a somatic hybrid derived from cytoplasmic male-sterile Brassica oleracea and atrazine-resistant B. campestris View Full Text


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Article Info

DATE

1987-07

AUTHORS

D. Robertson, J. D. Palmer, E. D. Earle, M. A. Mutschler

ABSTRACT

An atrazine-resistant, male-fertile Brassica napus plant was synthesized by fusion of protoplasts from the diploid species B. oleracea and B. campestris. Leaf protoplasts from B. oleracea var. italica carrying the Ogura male-sterile cytoplasm derived from Raphanus sativus were fused with etiolated hypocotyl protoplasts of atrazine-resistant B. campestris. The selection procedure was based on the inability of B. campestris protoplasts to regenerate in the media used, and the reduction of light-induced growth of B. oleracea tissue by atrazine. A somatic hybrid plant that differed in morphology from both B. oleracea and B. campestris was regenerated on medium containing 50 μM atrazine. Its chromosome number was 36-38, approximately that of B. napus. Furthermore, nuclear ribosomal DNA from this hybrid was a mixture of both parental rDNAs. Southern blot analyses of chloroplast DNA and an assay involving tetrazolium blue indicated that the hybrid contained atrazine-resistant B. campestris chloroplasts. The hybrid's mitochondrial genome was recombinant, containing fragments unique to each parent, as well as novel fragments carrying putative crossover points. Although the plant was female-sterile, it was successfully used to pollinate B. napus. More... »

PAGES

303-309

References to SciGraph publications

  • 1982-09. Mitochondrial DNA analyses of fertile and sterile maize plants derived from tissue culture with the texas male sterile cytoplasm in THEORETICAL AND APPLIED GENETICS
  • 1979-10. Mitochondrial recombination in cytoplasmic hybrids of Nicotiana tabacum by protoplast fusion in NATURE
  • 1985-03. A variant mitochondrial DNA arrangement specific toPetunia stable sterile somatic hybrids in PLANT MOLECULAR BIOLOGY
  • 1984-12. Cytoplasmic DNA variation in a potato protoclonal population in THEORETICAL AND APPLIED GENETICS
  • 1985-03. Organelle DNA compositions and isoenzyme expression in an interspecific somatic hybrid of Daucus in MOLECULAR GENETICS AND GENOMICS
  • 1983-09. A heteroplasmic state induced by protoplast fusion is a necessary condition for detecting rearrangements in Nicotiana mitochondrial DNA in THEORETICAL AND APPLIED GENETICS
  • 1985-07. Mitochondrial DNA polymorphism induced by protoplast fusion in Cruciferae in THEORETICAL AND APPLIED GENETICS
  • 1983-01. Detection of two different nuclear genomes in parasexual hybrids by ribosomal RNA gene analysis in THEORETICAL AND APPLIED GENETICS
  • 1983-06. Novel composition of mitochondrial genomes in Petunia somatic hybrids derived from cytoplasmic male sterile and fertile plants in MOLECULAR GENETICS AND GENOMICS
  • 1981-03. Mitochondrial DNA variation in maize plants regenerated during tissue culture selection in THEORETICAL AND APPLIED GENETICS
  • 1982-06. Cytoplasmic hybridization in Nicotiana: Mitochondrial DNA analysis in progenies resulting from fusion between protoplasts having different organelle constitutions in MOLECULAR GENETICS AND GENOMICS
  • 1983-06. Cybrids containing mixed and sorted-out chloroplasts following interspecific somatic fusions in Nicotiana in THEORETICAL AND APPLIED GENETICS
  • 1987-02. Nitro-blue tetrazolium: A specific stain for photosynthetic activity in protoplasts in PLANT CELL REPORTS
  • 1984-02. Tripartite structure of the Brassica campestris mitochondrial genome in NATURE
  • 1986-02. Plant regeneration from leaf protoplasts of Brassica oleracea var. italica CV Green Comet broccoli in PLANT CELL REPORTS
  • 1983-05. Chloroplast DNA evolution and the origin of amphidiploid Brassica species in THEORETICAL AND APPLIED GENETICS
  • 1983. The Segregation of Organelles and Cytoplasmic Traits in Higher Plant Somatic Fusion Hybrids in PROTOPLASTS 1983
  • 1983-08. Intergeneric cytoplasmic hybridization in cruciferae by protoplast fusion in MOLECULAR GENETICS AND GENOMICS
  • 1983-09. Response of light- and dark-grown callus of atrazine-resistant and susceptible rapeseed (Brassica napus) to varying concentrations of atrizine in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1985-07. Intergenomic recombination of mitochondrial genomes in a somatic hybrid plant in CURRENT GENETICS
  • Identifiers

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    http://scigraph.springernature.com/pub.10.1007/bf00274711

    DOI

    http://dx.doi.org/10.1007/bf00274711

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