Transposition of DNA inserted into deletions of the Tn5 kanamycin resistance element View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1979-01

AUTHORS

Richard Meyer, Gary Boch, James Shapiro

ABSTRACT

Tn5-trp hybrid transposons have been constructed by insertion of a trpPOED Hind III fragment into an in vivo Tn5 internal deletion mutant or by substitution of trp for the internal Tn5 Hind III fragment. These hybrids are called, respectively, Tn409 and Tn410. Both Tn409 and Tn410 will transpose into λ in the presence of a complementing Tn5 element. In the absence of a wild Tn5, lysogens carrying R1162::Tn409 and R1162::Tn410 plasmids will yield λtrp phages at less than six per cent of the complemented frequency. This reduction indicates that Tn409 and Tn410 lack a diffusible transposition function provided by wild Tn5 elements. However, the formation of λtrp phages without complementation is real. Most of these transducing particles contain Tn409 and Tn410 still linked to the carrier R1162 plasmid. This observation suggests that uncomplemented Tn409 and Tn410 elements mediate the formation of λ-transposon-plasmid cointegrate structures. Thus, the missing transposition function may be involved in resolving these cointegrate structures to the final λ::Tn409 or λ::Tn410 product. More... »

PAGES

7-13

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00274009

DOI

http://dx.doi.org/10.1007/bf00274009

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1026577966

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/375023


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Chromosome Mapping", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Coliphages", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA, Viral", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Drug Resistance, Microbial", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Escherichia coli", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genetic Complementation Test", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Kanamycin", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Lysogeny", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Operon", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "R Factors", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Recombination, Genetic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transduction, Genetic", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Department of Microbiology, University of Texas, Austin, Texas, USA", 
          "id": "http://www.grid.ac/institutes/grid.89336.37", 
          "name": [
            "Department of Microbiology, University of Chicago, 60637, Chicago, IL", 
            "Department of Microbiology, University of Texas, Austin, Texas, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Meyer", 
        "givenName": "Richard", 
        "id": "sg:person.01371653301.88", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01371653301.88"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Microbiology, University of Chicago, 60637, Chicago, IL", 
          "id": "http://www.grid.ac/institutes/grid.170205.1", 
          "name": [
            "Department of Microbiology, University of Chicago, 60637, Chicago, IL"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Boch", 
        "givenName": "Gary", 
        "id": "sg:person.0600437241.38", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0600437241.38"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Microbiology, University of Chicago, 60637, Chicago, IL", 
          "id": "http://www.grid.ac/institutes/grid.170205.1", 
          "name": [
            "Department of Microbiology, University of Chicago, 60637, Chicago, IL"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Shapiro", 
        "givenName": "James", 
        "id": "sg:person.01250033425.69", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01250033425.69"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/newbio242001a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009181475", 
          "https://doi.org/10.1038/newbio242001a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00268809", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036352663", 
          "https://doi.org/10.1007/bf00268809"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/256097a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024645284", 
          "https://doi.org/10.1038/256097a0"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1979-01", 
    "datePublishedReg": "1979-01-01", 
    "description": "Tn5-trp hybrid transposons have been constructed by insertion of a trpPOED Hind III fragment into an in vivo Tn5 internal deletion mutant or by substitution of trp for the internal Tn5 Hind III fragment. These hybrids are called, respectively, Tn409 and Tn410. Both Tn409 and Tn410 will transpose into \u03bb in the presence of a complementing Tn5 element. In the absence of a wild Tn5, lysogens carrying R1162::Tn409 and R1162::Tn410 plasmids will yield \u03bbtrp phages at less than six per cent of the complemented frequency. This reduction indicates that Tn409 and Tn410 lack a diffusible transposition function provided by wild Tn5 elements. However, the formation of \u03bbtrp phages without complementation is real. Most of these transducing particles contain Tn409 and Tn410 still linked to the carrier R1162 plasmid. This observation suggests that uncomplemented Tn409 and Tn410 elements mediate the formation of \u03bb-transposon-plasmid cointegrate structures. Thus, the missing transposition function may be involved in resolving these cointegrate structures to the final \u03bb::Tn409 or \u03bb::Tn410 product.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/bf00274009", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1297380", 
        "issn": [
          "1617-4615", 
          "1432-1874"
        ], 
        "name": "Molecular Genetics and Genomics", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "171"
      }
    ], 
    "keywords": [
      "Hind III fragment", 
      "internal deletion mutants", 
      "deletion mutants", 
      "substitution of Trp", 
      "Tn5 element", 
      "\u03bbtrp phages", 
      "transposition functions", 
      "cointegrate structure", 
      "transposition of DNA", 
      "kanamycin resistance element", 
      "transposon", 
      "fragments", 
      "mutants", 
      "Tn5", 
      "plasmid", 
      "phages", 
      "complementation", 
      "insertion", 
      "substitution", 
      "Trp", 
      "hybrids", 
      "elements", 
      "absence", 
      "lysogens", 
      "function", 
      "formation", 
      "structure", 
      "transposition", 
      "DNA", 
      "deletion", 
      "resistance elements", 
      "presence", 
      "frequency", 
      "reduction", 
      "particles", 
      "observations", 
      "products", 
      "cent"
    ], 
    "name": "Transposition of DNA inserted into deletions of the Tn5 kanamycin resistance element", 
    "pagination": "7-13", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1026577966"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/bf00274009"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "375023"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/bf00274009", 
      "https://app.dimensions.ai/details/publication/pub.1026577966"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-08-04T16:48", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220804/entities/gbq_results/article/article_108.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/bf00274009"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00274009'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00274009'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00274009'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00274009'


 

This table displays all metadata directly associated to this object as RDF triples.

177 TRIPLES      21 PREDICATES      79 URIs      68 LITERALS      19 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/bf00274009 schema:about N20e5bb4e6a484addbce8367d07cba122
2 N282c24a0a8c840a18d83151e693bc91f
3 N2c980dd7b7264b8a8d20acd619e69a17
4 N320e82de14084f38be14acc3189cc687
5 N41e2f98bb0a64efe8f7b95cdf5fa25a6
6 N58f4b6356d734019ade2300198642c97
7 N5bc4172ca82d41ce9068fd858d072b27
8 N7ba126cdcbce4855981a95162564189c
9 Na907ef7067a84541bcceba24de88ad47
10 Nadcce93da7e54afe9e00e9e80a5f84ac
11 Ne320be00f22d4f74926f85ee50d81f0e
12 Nee61ed6e624a486485b17d2bf371ffd4
13 anzsrc-for:06
14 anzsrc-for:0604
15 schema:author N7909c20c1b9b4935bbf4171926ba390d
16 schema:citation sg:pub.10.1007/bf00268809
17 sg:pub.10.1038/256097a0
18 sg:pub.10.1038/newbio242001a0
19 schema:datePublished 1979-01
20 schema:datePublishedReg 1979-01-01
21 schema:description Tn5-trp hybrid transposons have been constructed by insertion of a trpPOED Hind III fragment into an in vivo Tn5 internal deletion mutant or by substitution of trp for the internal Tn5 Hind III fragment. These hybrids are called, respectively, Tn409 and Tn410. Both Tn409 and Tn410 will transpose into λ in the presence of a complementing Tn5 element. In the absence of a wild Tn5, lysogens carrying R1162::Tn409 and R1162::Tn410 plasmids will yield λtrp phages at less than six per cent of the complemented frequency. This reduction indicates that Tn409 and Tn410 lack a diffusible transposition function provided by wild Tn5 elements. However, the formation of λtrp phages without complementation is real. Most of these transducing particles contain Tn409 and Tn410 still linked to the carrier R1162 plasmid. This observation suggests that uncomplemented Tn409 and Tn410 elements mediate the formation of λ-transposon-plasmid cointegrate structures. Thus, the missing transposition function may be involved in resolving these cointegrate structures to the final λ::Tn409 or λ::Tn410 product.
22 schema:genre article
23 schema:isAccessibleForFree false
24 schema:isPartOf N99f174d99af8436d839e2ecd82297159
25 Na4d265af5bee4cccb010e50982974ef7
26 sg:journal.1297380
27 schema:keywords DNA
28 Hind III fragment
29 Tn5
30 Tn5 element
31 Trp
32 absence
33 cent
34 cointegrate structure
35 complementation
36 deletion
37 deletion mutants
38 elements
39 formation
40 fragments
41 frequency
42 function
43 hybrids
44 insertion
45 internal deletion mutants
46 kanamycin resistance element
47 lysogens
48 mutants
49 observations
50 particles
51 phages
52 plasmid
53 presence
54 products
55 reduction
56 resistance elements
57 structure
58 substitution
59 substitution of Trp
60 transposition
61 transposition functions
62 transposition of DNA
63 transposon
64 λtrp phages
65 schema:name Transposition of DNA inserted into deletions of the Tn5 kanamycin resistance element
66 schema:pagination 7-13
67 schema:productId Na1c6e3d9b9324dc095e424de4b9a9086
68 Nc8092adce3e341ca9676977c5ec8ccfe
69 Nde294ffab9eb41dc944c644aecaa59d7
70 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026577966
71 https://doi.org/10.1007/bf00274009
72 schema:sdDatePublished 2022-08-04T16:48
73 schema:sdLicense https://scigraph.springernature.com/explorer/license/
74 schema:sdPublisher N7b443b40e1c540f0b0a5cd9cbb3433a5
75 schema:url https://doi.org/10.1007/bf00274009
76 sgo:license sg:explorer/license/
77 sgo:sdDataset articles
78 rdf:type schema:ScholarlyArticle
79 N20e5bb4e6a484addbce8367d07cba122 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
80 schema:name Lysogeny
81 rdf:type schema:DefinedTerm
82 N282c24a0a8c840a18d83151e693bc91f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
83 schema:name DNA, Viral
84 rdf:type schema:DefinedTerm
85 N2c980dd7b7264b8a8d20acd619e69a17 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
86 schema:name Recombination, Genetic
87 rdf:type schema:DefinedTerm
88 N320e82de14084f38be14acc3189cc687 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
89 schema:name Drug Resistance, Microbial
90 rdf:type schema:DefinedTerm
91 N41e2f98bb0a64efe8f7b95cdf5fa25a6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
92 schema:name Coliphages
93 rdf:type schema:DefinedTerm
94 N48513fd7195b4c5a80c7c030c2670ef8 rdf:first sg:person.0600437241.38
95 rdf:rest Ncbd30e07e75e4ca08bee8a70247f6e32
96 N58f4b6356d734019ade2300198642c97 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
97 schema:name Escherichia coli
98 rdf:type schema:DefinedTerm
99 N5bc4172ca82d41ce9068fd858d072b27 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
100 schema:name Kanamycin
101 rdf:type schema:DefinedTerm
102 N7909c20c1b9b4935bbf4171926ba390d rdf:first sg:person.01371653301.88
103 rdf:rest N48513fd7195b4c5a80c7c030c2670ef8
104 N7b443b40e1c540f0b0a5cd9cbb3433a5 schema:name Springer Nature - SN SciGraph project
105 rdf:type schema:Organization
106 N7ba126cdcbce4855981a95162564189c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
107 schema:name R Factors
108 rdf:type schema:DefinedTerm
109 N99f174d99af8436d839e2ecd82297159 schema:issueNumber 1
110 rdf:type schema:PublicationIssue
111 Na1c6e3d9b9324dc095e424de4b9a9086 schema:name dimensions_id
112 schema:value pub.1026577966
113 rdf:type schema:PropertyValue
114 Na4d265af5bee4cccb010e50982974ef7 schema:volumeNumber 171
115 rdf:type schema:PublicationVolume
116 Na907ef7067a84541bcceba24de88ad47 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
117 schema:name Transduction, Genetic
118 rdf:type schema:DefinedTerm
119 Nadcce93da7e54afe9e00e9e80a5f84ac schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
120 schema:name Operon
121 rdf:type schema:DefinedTerm
122 Nc8092adce3e341ca9676977c5ec8ccfe schema:name pubmed_id
123 schema:value 375023
124 rdf:type schema:PropertyValue
125 Ncbd30e07e75e4ca08bee8a70247f6e32 rdf:first sg:person.01250033425.69
126 rdf:rest rdf:nil
127 Nde294ffab9eb41dc944c644aecaa59d7 schema:name doi
128 schema:value 10.1007/bf00274009
129 rdf:type schema:PropertyValue
130 Ne320be00f22d4f74926f85ee50d81f0e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
131 schema:name Genetic Complementation Test
132 rdf:type schema:DefinedTerm
133 Nee61ed6e624a486485b17d2bf371ffd4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
134 schema:name Chromosome Mapping
135 rdf:type schema:DefinedTerm
136 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
137 schema:name Biological Sciences
138 rdf:type schema:DefinedTerm
139 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
140 schema:name Genetics
141 rdf:type schema:DefinedTerm
142 sg:journal.1297380 schema:issn 1432-1874
143 1617-4615
144 schema:name Molecular Genetics and Genomics
145 schema:publisher Springer Nature
146 rdf:type schema:Periodical
147 sg:person.01250033425.69 schema:affiliation grid-institutes:grid.170205.1
148 schema:familyName Shapiro
149 schema:givenName James
150 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01250033425.69
151 rdf:type schema:Person
152 sg:person.01371653301.88 schema:affiliation grid-institutes:grid.89336.37
153 schema:familyName Meyer
154 schema:givenName Richard
155 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01371653301.88
156 rdf:type schema:Person
157 sg:person.0600437241.38 schema:affiliation grid-institutes:grid.170205.1
158 schema:familyName Boch
159 schema:givenName Gary
160 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0600437241.38
161 rdf:type schema:Person
162 sg:pub.10.1007/bf00268809 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036352663
163 https://doi.org/10.1007/bf00268809
164 rdf:type schema:CreativeWork
165 sg:pub.10.1038/256097a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024645284
166 https://doi.org/10.1038/256097a0
167 rdf:type schema:CreativeWork
168 sg:pub.10.1038/newbio242001a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009181475
169 https://doi.org/10.1038/newbio242001a0
170 rdf:type schema:CreativeWork
171 grid-institutes:grid.170205.1 schema:alternateName Department of Microbiology, University of Chicago, 60637, Chicago, IL
172 schema:name Department of Microbiology, University of Chicago, 60637, Chicago, IL
173 rdf:type schema:Organization
174 grid-institutes:grid.89336.37 schema:alternateName Department of Microbiology, University of Texas, Austin, Texas, USA
175 schema:name Department of Microbiology, University of Chicago, 60637, Chicago, IL
176 Department of Microbiology, University of Texas, Austin, Texas, USA
177 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...