PCR based gene engineering of the Vibrio harveyi lux operon and the Escherichia coli trp operon provides for biochemically functional ... View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1991-04

AUTHORS

Philip J. Hill, Simon Swift, Gordon S. A. B. Stewart

ABSTRACT

The polymerase chain reaction (PCR) was applied to clone the luxA and luxB genes from Vibrio harveyi, and the trp poL (promoter operator leader) region and the trpB and trpA genes from Escherichia coli. PCR-derived luxA/B and trpB/A genes were shown to express bacterial luciferase and tryptophan synthase respectively, when introduced into E. coli on a plasmid cloning vehicle. The trp poL was used successfully to control the expression of lac alpha, luxAB, trpB and trpA. PCR was also used to construct a functional luxAB translational fusion protein. Primers for this were designed to facilitate precise gene fusion and to provide a silent mutation within an EcoRI site in the luxB gene. Production of functional genes was verified in vitro and in vivo using polyacrylamide gel electrophoresis (PAGE) analysis of transcription-translation products and crude cell extracts, and by monitoring enzyme activity. More... »

PAGES

41-48

References to SciGraph publications

  • 1981-02. Attenuation in the control of expression of bacterial operons in NATURE
  • 1989-04. New Clues on Folding in NATURE BIOTECHNOLOGY
  • Journal

    TITLE

    Molecular Genetics and Genomics

    ISSUE

    1-2

    VOLUME

    226

    Author Affiliations

    Related Patents

  • Microorganisms And Methods For Conversion Of Syngas And Other Carbon Sources To Useful Products
  • Microorganisms For The Production Of Methacrylic Acid
  • Semi-Synthetic Terephthalic Acid Via Microorganisms That Produce Muconic Acid
  • Microorganisms And Methods For The Co-Production Of Isopropanol And 1,4-Butanediol
  • Methods And Organisms For Converting Synthesis Gas Or Other Gaseous Carbon Sources And Methanol To 1,3-Butanediol
  • Microorganisms And Methods For Carbon-Efficient Biosynthesis Of Mek And 2-Butanol
  • Methods For The Synthesis Of Olefins And Derivatives
  • Microorganisms For The Production Of Adipic Acid And Other Compounds
  • Methods And Organisms For Utilizing Synthesis Gas Or Other Gaseous Carbon Sources And Methanol
  • Microorganisms And Methods For The Biosynthesis Of Butadiene
  • Microorganisms And Methods For The Biosynthesis Of Propylene
  • Semi-Synthetic Terephthalic Acid Via Microorganisms That Produce Muconic Acid
  • Microorganisms And Methods For The Biosynthesis Of Adipate, Hexamethylenediamine And 6-Aminocaproic Acid
  • Organisms For The Production Of 1,3-Butanediol
  • Microorganisms And Methods For The Biosynthesis Of Aromatics, 2,4-Pentadienoate And 1,3-Butadiene
  • Microorganisms And Methods For The Biosynthesis Of Fumarate, Malate, And Acrylate
  • Methods And Organisms For Utilizing Synthesis Gas Or Other Gaseous Carbon Sources And Methanol
  • Microbial Organisms Comprising Exogenous Nucleic Acids Encoding Reductive Tca Pathway Enzymes
  • Microorganisms For The Production Of Adipic Acid And Other Compounds
  • Methods And Organisms For Utilizing Synthesis Gas Or Other Gaseous Carbon Sources And Methanol
  • Methods For Increasing Product Yields
  • Microorganisms For The Production Of 1,4-Butanediol, 4-Hydroxybutanal, 4-Hydroxybutyryl-Coa, Putrescine And Related Compounds, And Methods Related Thereto
  • Primary Alcohol Producing Organisms
  • Methods And Organisms For Utilizing Synthesis Gas Or Other Gaseous Carbon Sources And Methanol
  • Organisms For The Production Of Isopropanol, N-Butanol, And Isobutanol
  • Methods And Organisms For Utilizing Synthesis Gas Or Other Gaseous Carbon Sources And Methanol
  • Microorganisms For The Production Of Adipic Acid And Other Compounds
  • Primary Alcohol Producing Organisms
  • Methods And Organisms For Utilizing Synthesis Gas Or Other Gaseous Carbon Sources And Methanol
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00273585

    DOI

    http://dx.doi.org/10.1007/bf00273585

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1031936899

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/2034229


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Base Sequence", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cloning, Molecular", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "DNA, Bacterial", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Electrophoresis, Polyacrylamide Gel", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Escherichia coli", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Expression Regulation, Bacterial", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genes, Bacterial", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Luciferases", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Molecular Sequence Data", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Operator Regions, Genetic", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Operon", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Polymerase Chain Reaction", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Promoter Regions, Genetic", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Recombinant Fusion Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Tryptophan Synthase", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Vibrio", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "University of Nottingham", 
              "id": "https://www.grid.ac/institutes/grid.4563.4", 
              "name": [
                "Department of Applied Biochemistry and Food Science, University of Nottingham School of Agriculture, LE12 SRD, Sutton Bonington, Leicestershire, UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Hill", 
            "givenName": "Philip J.", 
            "id": "sg:person.01202710675.32", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01202710675.32"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Nottingham", 
              "id": "https://www.grid.ac/institutes/grid.4563.4", 
              "name": [
                "Department of Applied Biochemistry and Food Science, University of Nottingham School of Agriculture, LE12 SRD, Sutton Bonington, Leicestershire, UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Swift", 
            "givenName": "Simon", 
            "id": "sg:person.012212615125.67", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012212615125.67"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Nottingham", 
              "id": "https://www.grid.ac/institutes/grid.4563.4", 
              "name": [
                "Department of Applied Biochemistry and Food Science, University of Nottingham School of Agriculture, LE12 SRD, Sutton Bonington, Leicestershire, UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Stewart", 
            "givenName": "Gordon S. A. B.", 
            "id": "sg:person.011512127722.09", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011512127722.09"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1146/annurev.mi.42.100188.001055", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001765464"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/9780470122945.ch4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003396308"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nbt0489-324", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011216753", 
              "https://doi.org/10.1038/nbt0489-324"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/16.21.10393", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014300700"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1472-765x.1989.tb00311.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014950939"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(85)90120-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017120147"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(85)90120-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017120147"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1472-765x.1990.tb00082.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020221431"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0147-619x(86)90035-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020368631"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1080/02648725.1988.10647844", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021067940"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/bio.1170050204", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021234584"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/bio.1170050204", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021234584"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(89)90358-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021797759"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(89)90358-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1021797759"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1365-2958.1989.tb00252.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022935259"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1432-1033.1975.tb21030.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023406164"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.86.17.6528", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031266292"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0076-6879(87)42051-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033750621"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/bio.1170040143", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039486574"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/bio.1170040143", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039486574"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.44.12.1161", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039820699"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/16.24.11820", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046956190"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(89)90359-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048752288"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(89)90359-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048752288"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(89)90195-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050108028"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(89)90195-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050108028"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.81.13.4154", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050938231"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(89)90194-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051665492"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(89)90194-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051665492"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.85.24.9436", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053324077"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/289751a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053330653", 
              "https://doi.org/10.1038/289751a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/bi00416a027", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055174871"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1099/00221287-135-4-903", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1060367303"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.2448875", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062538000"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/jb.171.5.2466-2473.1989", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062717857"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1078430400", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1079004740", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1079161383", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1079527749", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1080025695", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1080446060", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1080471333", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1081003071", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.239.4839.487", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1101677454"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "1991-04", 
        "datePublishedReg": "1991-04-01", 
        "description": "The polymerase chain reaction (PCR) was applied to clone the luxA and luxB genes from Vibrio harveyi, and the trp poL (promoter operator leader) region and the trpB and trpA genes from Escherichia coli. PCR-derived luxA/B and trpB/A genes were shown to express bacterial luciferase and tryptophan synthase respectively, when introduced into E. coli on a plasmid cloning vehicle. The trp poL was used successfully to control the expression of lac alpha, luxAB, trpB and trpA. PCR was also used to construct a functional luxAB translational fusion protein. Primers for this were designed to facilitate precise gene fusion and to provide a silent mutation within an EcoRI site in the luxB gene. Production of functional genes was verified in vitro and in vivo using polyacrylamide gel electrophoresis (PAGE) analysis of transcription-translation products and crude cell extracts, and by monitoring enzyme activity.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1007/bf00273585", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1297380", 
            "issn": [
              "1617-4615", 
              "1432-1874"
            ], 
            "name": "Molecular Genetics and Genomics", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "1-2", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "226"
          }
        ], 
        "name": "PCR based gene engineering of the Vibrio harveyi lux operon and the Escherichia coli trp operon provides for biochemically functional native and fused gene products", 
        "pagination": "41-48", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "982441a46763671616e7a978c08872146746762b603c700c98a61ddb69ded3e6"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "2034229"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "0125036"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/bf00273585"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1031936899"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/bf00273585", 
          "https://app.dimensions.ai/details/publication/pub.1031936899"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-11T13:51", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000371_0000000371/records_130801_00000002.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://link.springer.com/10.1007/BF00273585"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00273585'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00273585'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00273585'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00273585'


     

    This table displays all metadata directly associated to this object as RDF triples.

    252 TRIPLES      21 PREDICATES      82 URIs      37 LITERALS      25 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/bf00273585 schema:about N0d1939403c8c4fae9738e3efaca6d05e
    2 N237be10246f242a884a230698b22dcd8
    3 N24a7f903d44445a48ef65934d3571581
    4 N24bf2c947e4048ff90fb25e19236b51d
    5 N35cafa30dead4b349e0af78cab0e84b9
    6 N42b0c3b1046546c3b8f3814f9ba4eed5
    7 N53355aed5b0c43129756a40473d2eb66
    8 N5812b56abaab49aa9bf8e138e707b633
    9 N58c2f3ae23a547f1949b1e6d9621487a
    10 N609cc880df184eeabd7a722954fb9329
    11 N7e262bfd61af4de9897d26a253847a91
    12 N967c8284cb14479ca8955f923c806551
    13 Na5953fb3f1bf49cc91da5c7f7c6b370f
    14 Naa1ba61a359e46afab6ae2b128c2e434
    15 Nb0f4a22640aa47a598f14d79524e301f
    16 Ndef21fccb55f4bd598e47e39b308c3c4
    17 anzsrc-for:06
    18 anzsrc-for:0604
    19 schema:author N1afa3aa5a4994f5e8a8e6e0f52dbaa60
    20 schema:citation sg:pub.10.1038/289751a0
    21 sg:pub.10.1038/nbt0489-324
    22 https://app.dimensions.ai/details/publication/pub.1078430400
    23 https://app.dimensions.ai/details/publication/pub.1079004740
    24 https://app.dimensions.ai/details/publication/pub.1079161383
    25 https://app.dimensions.ai/details/publication/pub.1079527749
    26 https://app.dimensions.ai/details/publication/pub.1080025695
    27 https://app.dimensions.ai/details/publication/pub.1080446060
    28 https://app.dimensions.ai/details/publication/pub.1080471333
    29 https://app.dimensions.ai/details/publication/pub.1081003071
    30 https://doi.org/10.1002/9780470122945.ch4
    31 https://doi.org/10.1002/bio.1170040143
    32 https://doi.org/10.1002/bio.1170050204
    33 https://doi.org/10.1016/0147-619x(86)90035-1
    34 https://doi.org/10.1016/0378-1119(85)90120-9
    35 https://doi.org/10.1016/0378-1119(89)90194-7
    36 https://doi.org/10.1016/0378-1119(89)90195-9
    37 https://doi.org/10.1016/0378-1119(89)90358-2
    38 https://doi.org/10.1016/0378-1119(89)90359-4
    39 https://doi.org/10.1016/s0076-6879(87)42051-x
    40 https://doi.org/10.1021/bi00416a027
    41 https://doi.org/10.1073/pnas.44.12.1161
    42 https://doi.org/10.1073/pnas.81.13.4154
    43 https://doi.org/10.1073/pnas.85.24.9436
    44 https://doi.org/10.1073/pnas.86.17.6528
    45 https://doi.org/10.1080/02648725.1988.10647844
    46 https://doi.org/10.1093/nar/16.21.10393
    47 https://doi.org/10.1093/nar/16.24.11820
    48 https://doi.org/10.1099/00221287-135-4-903
    49 https://doi.org/10.1111/j.1365-2958.1989.tb00252.x
    50 https://doi.org/10.1111/j.1432-1033.1975.tb21030.x
    51 https://doi.org/10.1111/j.1472-765x.1989.tb00311.x
    52 https://doi.org/10.1111/j.1472-765x.1990.tb00082.x
    53 https://doi.org/10.1126/science.239.4839.487
    54 https://doi.org/10.1126/science.2448875
    55 https://doi.org/10.1128/jb.171.5.2466-2473.1989
    56 https://doi.org/10.1146/annurev.mi.42.100188.001055
    57 schema:datePublished 1991-04
    58 schema:datePublishedReg 1991-04-01
    59 schema:description The polymerase chain reaction (PCR) was applied to clone the luxA and luxB genes from Vibrio harveyi, and the trp poL (promoter operator leader) region and the trpB and trpA genes from Escherichia coli. PCR-derived luxA/B and trpB/A genes were shown to express bacterial luciferase and tryptophan synthase respectively, when introduced into E. coli on a plasmid cloning vehicle. The trp poL was used successfully to control the expression of lac alpha, luxAB, trpB and trpA. PCR was also used to construct a functional luxAB translational fusion protein. Primers for this were designed to facilitate precise gene fusion and to provide a silent mutation within an EcoRI site in the luxB gene. Production of functional genes was verified in vitro and in vivo using polyacrylamide gel electrophoresis (PAGE) analysis of transcription-translation products and crude cell extracts, and by monitoring enzyme activity.
    60 schema:genre research_article
    61 schema:inLanguage en
    62 schema:isAccessibleForFree false
    63 schema:isPartOf N9dbafce147cc45ba82f2b40175d47526
    64 Na769a72eca8549e598e3da1cde532078
    65 sg:journal.1297380
    66 schema:name PCR based gene engineering of the Vibrio harveyi lux operon and the Escherichia coli trp operon provides for biochemically functional native and fused gene products
    67 schema:pagination 41-48
    68 schema:productId N3d9c9e7e2d97400f90bf12af125096b6
    69 N4299fecd3b1d47a294cca2e9df2675ff
    70 Na92f02ebed0e4154a93a9baf2d06a867
    71 Ncb50b9988d6140e19f071966b938fccd
    72 Nf32b95118c4f499eb7448f20ee5e8fc5
    73 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031936899
    74 https://doi.org/10.1007/bf00273585
    75 schema:sdDatePublished 2019-04-11T13:51
    76 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    77 schema:sdPublisher N287ef677d3c247c5a10faaa9bac6005a
    78 schema:url http://link.springer.com/10.1007/BF00273585
    79 sgo:license sg:explorer/license/
    80 sgo:sdDataset articles
    81 rdf:type schema:ScholarlyArticle
    82 N0d1939403c8c4fae9738e3efaca6d05e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    83 schema:name Molecular Sequence Data
    84 rdf:type schema:DefinedTerm
    85 N1afa3aa5a4994f5e8a8e6e0f52dbaa60 rdf:first sg:person.01202710675.32
    86 rdf:rest N68092595a4b14b098bf92f4da3c64e7f
    87 N237be10246f242a884a230698b22dcd8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    88 schema:name Escherichia coli
    89 rdf:type schema:DefinedTerm
    90 N24a7f903d44445a48ef65934d3571581 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    91 schema:name Base Sequence
    92 rdf:type schema:DefinedTerm
    93 N24bf2c947e4048ff90fb25e19236b51d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    94 schema:name Electrophoresis, Polyacrylamide Gel
    95 rdf:type schema:DefinedTerm
    96 N287ef677d3c247c5a10faaa9bac6005a schema:name Springer Nature - SN SciGraph project
    97 rdf:type schema:Organization
    98 N35cafa30dead4b349e0af78cab0e84b9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    99 schema:name Operator Regions, Genetic
    100 rdf:type schema:DefinedTerm
    101 N3d9c9e7e2d97400f90bf12af125096b6 schema:name dimensions_id
    102 schema:value pub.1031936899
    103 rdf:type schema:PropertyValue
    104 N4299fecd3b1d47a294cca2e9df2675ff schema:name pubmed_id
    105 schema:value 2034229
    106 rdf:type schema:PropertyValue
    107 N42b0c3b1046546c3b8f3814f9ba4eed5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    108 schema:name Polymerase Chain Reaction
    109 rdf:type schema:DefinedTerm
    110 N53355aed5b0c43129756a40473d2eb66 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    111 schema:name Vibrio
    112 rdf:type schema:DefinedTerm
    113 N5812b56abaab49aa9bf8e138e707b633 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    114 schema:name Genes, Bacterial
    115 rdf:type schema:DefinedTerm
    116 N58c2f3ae23a547f1949b1e6d9621487a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    117 schema:name Operon
    118 rdf:type schema:DefinedTerm
    119 N609cc880df184eeabd7a722954fb9329 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    120 schema:name Tryptophan Synthase
    121 rdf:type schema:DefinedTerm
    122 N68092595a4b14b098bf92f4da3c64e7f rdf:first sg:person.012212615125.67
    123 rdf:rest Nef1bd21f305c452483a3a973f52fce7c
    124 N7e262bfd61af4de9897d26a253847a91 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    125 schema:name Luciferases
    126 rdf:type schema:DefinedTerm
    127 N967c8284cb14479ca8955f923c806551 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    128 schema:name Promoter Regions, Genetic
    129 rdf:type schema:DefinedTerm
    130 N9dbafce147cc45ba82f2b40175d47526 schema:issueNumber 1-2
    131 rdf:type schema:PublicationIssue
    132 Na5953fb3f1bf49cc91da5c7f7c6b370f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    133 schema:name Gene Expression Regulation, Bacterial
    134 rdf:type schema:DefinedTerm
    135 Na769a72eca8549e598e3da1cde532078 schema:volumeNumber 226
    136 rdf:type schema:PublicationVolume
    137 Na92f02ebed0e4154a93a9baf2d06a867 schema:name readcube_id
    138 schema:value 982441a46763671616e7a978c08872146746762b603c700c98a61ddb69ded3e6
    139 rdf:type schema:PropertyValue
    140 Naa1ba61a359e46afab6ae2b128c2e434 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    141 schema:name Cloning, Molecular
    142 rdf:type schema:DefinedTerm
    143 Nb0f4a22640aa47a598f14d79524e301f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    144 schema:name Recombinant Fusion Proteins
    145 rdf:type schema:DefinedTerm
    146 Ncb50b9988d6140e19f071966b938fccd schema:name nlm_unique_id
    147 schema:value 0125036
    148 rdf:type schema:PropertyValue
    149 Ndef21fccb55f4bd598e47e39b308c3c4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    150 schema:name DNA, Bacterial
    151 rdf:type schema:DefinedTerm
    152 Nef1bd21f305c452483a3a973f52fce7c rdf:first sg:person.011512127722.09
    153 rdf:rest rdf:nil
    154 Nf32b95118c4f499eb7448f20ee5e8fc5 schema:name doi
    155 schema:value 10.1007/bf00273585
    156 rdf:type schema:PropertyValue
    157 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    158 schema:name Biological Sciences
    159 rdf:type schema:DefinedTerm
    160 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    161 schema:name Genetics
    162 rdf:type schema:DefinedTerm
    163 sg:journal.1297380 schema:issn 1432-1874
    164 1617-4615
    165 schema:name Molecular Genetics and Genomics
    166 rdf:type schema:Periodical
    167 sg:person.011512127722.09 schema:affiliation https://www.grid.ac/institutes/grid.4563.4
    168 schema:familyName Stewart
    169 schema:givenName Gordon S. A. B.
    170 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011512127722.09
    171 rdf:type schema:Person
    172 sg:person.01202710675.32 schema:affiliation https://www.grid.ac/institutes/grid.4563.4
    173 schema:familyName Hill
    174 schema:givenName Philip J.
    175 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01202710675.32
    176 rdf:type schema:Person
    177 sg:person.012212615125.67 schema:affiliation https://www.grid.ac/institutes/grid.4563.4
    178 schema:familyName Swift
    179 schema:givenName Simon
    180 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012212615125.67
    181 rdf:type schema:Person
    182 sg:pub.10.1038/289751a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053330653
    183 https://doi.org/10.1038/289751a0
    184 rdf:type schema:CreativeWork
    185 sg:pub.10.1038/nbt0489-324 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011216753
    186 https://doi.org/10.1038/nbt0489-324
    187 rdf:type schema:CreativeWork
    188 https://app.dimensions.ai/details/publication/pub.1078430400 schema:CreativeWork
    189 https://app.dimensions.ai/details/publication/pub.1079004740 schema:CreativeWork
    190 https://app.dimensions.ai/details/publication/pub.1079161383 schema:CreativeWork
    191 https://app.dimensions.ai/details/publication/pub.1079527749 schema:CreativeWork
    192 https://app.dimensions.ai/details/publication/pub.1080025695 schema:CreativeWork
    193 https://app.dimensions.ai/details/publication/pub.1080446060 schema:CreativeWork
    194 https://app.dimensions.ai/details/publication/pub.1080471333 schema:CreativeWork
    195 https://app.dimensions.ai/details/publication/pub.1081003071 schema:CreativeWork
    196 https://doi.org/10.1002/9780470122945.ch4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003396308
    197 rdf:type schema:CreativeWork
    198 https://doi.org/10.1002/bio.1170040143 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039486574
    199 rdf:type schema:CreativeWork
    200 https://doi.org/10.1002/bio.1170050204 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021234584
    201 rdf:type schema:CreativeWork
    202 https://doi.org/10.1016/0147-619x(86)90035-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020368631
    203 rdf:type schema:CreativeWork
    204 https://doi.org/10.1016/0378-1119(85)90120-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017120147
    205 rdf:type schema:CreativeWork
    206 https://doi.org/10.1016/0378-1119(89)90194-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051665492
    207 rdf:type schema:CreativeWork
    208 https://doi.org/10.1016/0378-1119(89)90195-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050108028
    209 rdf:type schema:CreativeWork
    210 https://doi.org/10.1016/0378-1119(89)90358-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021797759
    211 rdf:type schema:CreativeWork
    212 https://doi.org/10.1016/0378-1119(89)90359-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048752288
    213 rdf:type schema:CreativeWork
    214 https://doi.org/10.1016/s0076-6879(87)42051-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1033750621
    215 rdf:type schema:CreativeWork
    216 https://doi.org/10.1021/bi00416a027 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055174871
    217 rdf:type schema:CreativeWork
    218 https://doi.org/10.1073/pnas.44.12.1161 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039820699
    219 rdf:type schema:CreativeWork
    220 https://doi.org/10.1073/pnas.81.13.4154 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050938231
    221 rdf:type schema:CreativeWork
    222 https://doi.org/10.1073/pnas.85.24.9436 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053324077
    223 rdf:type schema:CreativeWork
    224 https://doi.org/10.1073/pnas.86.17.6528 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031266292
    225 rdf:type schema:CreativeWork
    226 https://doi.org/10.1080/02648725.1988.10647844 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021067940
    227 rdf:type schema:CreativeWork
    228 https://doi.org/10.1093/nar/16.21.10393 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014300700
    229 rdf:type schema:CreativeWork
    230 https://doi.org/10.1093/nar/16.24.11820 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046956190
    231 rdf:type schema:CreativeWork
    232 https://doi.org/10.1099/00221287-135-4-903 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060367303
    233 rdf:type schema:CreativeWork
    234 https://doi.org/10.1111/j.1365-2958.1989.tb00252.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1022935259
    235 rdf:type schema:CreativeWork
    236 https://doi.org/10.1111/j.1432-1033.1975.tb21030.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1023406164
    237 rdf:type schema:CreativeWork
    238 https://doi.org/10.1111/j.1472-765x.1989.tb00311.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1014950939
    239 rdf:type schema:CreativeWork
    240 https://doi.org/10.1111/j.1472-765x.1990.tb00082.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1020221431
    241 rdf:type schema:CreativeWork
    242 https://doi.org/10.1126/science.239.4839.487 schema:sameAs https://app.dimensions.ai/details/publication/pub.1101677454
    243 rdf:type schema:CreativeWork
    244 https://doi.org/10.1126/science.2448875 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062538000
    245 rdf:type schema:CreativeWork
    246 https://doi.org/10.1128/jb.171.5.2466-2473.1989 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062717857
    247 rdf:type schema:CreativeWork
    248 https://doi.org/10.1146/annurev.mi.42.100188.001055 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001765464
    249 rdf:type schema:CreativeWork
    250 https://www.grid.ac/institutes/grid.4563.4 schema:alternateName University of Nottingham
    251 schema:name Department of Applied Biochemistry and Food Science, University of Nottingham School of Agriculture, LE12 SRD, Sutton Bonington, Leicestershire, UK
    252 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...