Introduction of foreign genes into potato cultivars Bintje and Désirée using an Agrobacterium tumefaciens binary vector View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1988-01

AUTHORS

Willem J. Stiekema, Freek Heidekamp, Jeanine D. Louwerse, Harrie A. Verhoeven, Paul Dijkhuis

ABSTRACT

Tuber discs of Solanum tuberosum cv Bintje and Désirée were cocultivated with an Agrobacterium tumefaciens binary vector, carrying both the neomycine phosphotransferase and the E. coli β-glucuronidase gene fused to resp. the nopaline synthase and Cauliflower mosaic virus 35S promotor.Inoculated tuber discs produce transgenic shoots in selective media containing kanamycin. The transgenic plants are phenotypically normal and contain the euploid number of chromosomes. Both the neomycin phosphotransferase as well as the β-glucuronidase gene are expressed conferring resp. kanamycin resistance and β-glucuronidase activity to the plants. More... »

PAGES

47-50

References to SciGraph publications

Journal

TITLE

Plant Cell Reports

ISSUE

1

VOLUME

7

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00272976

DOI

http://dx.doi.org/10.1007/bf00272976

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1027324923

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24241414


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