High efficiency Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana leaf and cotyledon explants View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1988-12

AUTHORS

Renate Schmidt, Lothar Willmitzer

ABSTRACT

A highly efficient and fast Agrobacterium-mediated leaf disc transformation system for the Arabidopsis thaliana L. genotype C24 was developed. This protocol is also amenable to other ecotypes - as could be shown for Landsberg erecta and Wassllewskija. Besides the hygromycin selection also the G418 and kanamycin selection were established. Furthermore the described procedure is appliable not only to leaf explants but also to expanded cotyledons which proved to be an excellent alternative as explant source for transformation experiments. More... »

PAGES

583-586

Journal

TITLE

Plant Cell Reports

ISSUE

7

VOLUME

7

Related Patents

  • Method Of Reducing Gene Expression Using Modified Codon Usage
  • For Introducing Dna Into Organisms Which Produce Large Amounts Of Oils; Genetic Engineering; For Animal Feed, Foods, Cosmetics And Pharmaceuticals; Kits
  • Method For Producing Aminoacids
  • Method For Producing Polyunsaturated C20 And C22 Fatty Acids With At Least Four Double Bonds In Transgenic Plants
  • Transcription Factor Stress-Related Proteins And Methods Of Use In Plants
  • Increase In Root Length In Arabidopsis Plants Over-Expressing The Arabidopsis Shmt4 (Shm4) Gene (At4g13930)
  • Method For Producing Polyunsaturated Fatty Acids In Transgenic Organisms
  • Method For The Production Of Polyunsaturated Fatty Acids
  • Process For The Production Of Delta5-Unsaturated Fatty Acids In Transgenic Organisms
  • Method For Producing Polyunsaturated Fatty Acids In Transgenic Organisms
  • Manipulation Of The Nitrogen Metabolism Using Ammonium Transporter Or Glucose 6-Phosphate Deshydrogenases Or Farnesyl Phosphate Synthetase (Fpp)
  • Increased Yield Plants By Increasing Or Generating One Or More Activities In A Plant Or Part Thereof
  • Yield Increase In Plants Overexpressing The Accdp Genes
  • Nucleic Acid
  • Nucleic Acid Sequences Encoding Proteins Associated With Abiotic Stress Response, And Transgenic Plant Cells Transformed With The Same
  • Corynebacterium Glutamicum Genes Encoding Proteins Involved In Carbon Metabolism And Energy Production
  • Transcription Factor Stress-Related Polypeptides And Methods Of Use In Plants
  • Desaturases And Process For The Production Of Polyunsaturated Fatty Acids In Transgenic Organisms
  • Method For Producing Polyunsatured Fatty Acids In Transgenic Organisms
  • Genes Coding For Proteins Of Carbon Metabolism And Energy Production
  • Method For Production Of Polyunsaturated Fatty Acids In Transgenic Organisms
  • Processes For Producing Polyunsaturated Fatty Acids In Transgenic Organisms
  • Method For Producing Polyunsaturated Fatty Acids In Transgenic Non-Human Organisms
  • Yield Increase In Plants Overexpressing The Shsrp Genes
  • New Vegetable Acyltransferases Specifically For Long-Chain Polyunsaturated Fatty Acids
  • Methods For Conversion Of The Substrate Specificity Of Desaturases
  • Protein Kinase Stress-Related Proteins And Methods Of Use In Plants
  • Plants With Increased Tolerance And/Or Resistance To Environmental Stress And Increased Biomass Production
  • Processes For Producing Polyunsaturated Fatty Acids In Transgenic Organisms
  • Method For Producing Unsaturated Omega-3 Fatty Acids In Transgenic Organisms
  • Gene Variants Coding For Proteins From The Metabolic Pathway Of Fine Chemicals
  • Nucleic Acid Sequences Encoding Proteins Associated With Abiotic Stress Response And Plant Cells And Plants With Increased Tolerance To Environmental Stress
  • Genes Which Encode Proteins For Glucose-6-Phosphate-Dehydrogenase
  • Process For The Production Of A Fine Chemical
  • Method For Producing Polyunsaturated Fatty Acids In Transgenic Organisms
  • Method For Producing Polyunsaturated Fatty Acids In Transgenic Non-Human Organisms
  • Process For The Control Of Production Of Fine Chemicals
  • Method For Production Of Polyunsaturated Fatty Acids In Transgenic Organisms
  • Process For The Production Of Fine Chemicals
  • Nucleic Acid Sequence Encoding The Opca Gene
  • New Vegetable Acyltransferases Specifically For Long-Chain Polyunsaturated Fatty Acids
  • Corynebacterium Glutamicum Gene Encoding Phosphoenolpyruvate-Protein Phosphotransferase
  • Process For The Production Of Delta5-Unsaturated Fatty Acids In Transgenic Organisms
  • Processes For Producing Polyunsaturated Fatty Acids In Transgenic Organisms
  • Corynebacterium Glutamicum Gene Encoding Hpr Of Phosphoenolpyruvate:Sugar Phosphotransferase System
  • Process For Decreasing Verbascose In A Plant By Expression Of A Chloroplast-Targeted Fimd Protein
  • Proteins Associated With Abiotic Stress Response And Homologs
  • Process For The Production Of Fine Chemicals
  • Process For The Production Of Fine Chemicals
  • Processes For Producing Polyunsaturated Fatty Acids In Transgenic Organisms
  • Corynebacterium Glutamicum Gene Encoding Phosphoenolpyruvate Carboxykinase
  • Method For Production Of Polyunsaturated Fatty Acids In Transgene Organisms
  • Manipulation Of The Nitrogen Metabolism
  • Desaturases And Methods Of Producing Polyunsaturated Fatty Acids In Transgenic Organisms
  • Process For The Production Of Fine Chemicals
  • Process For The Production Of Fine Chemicals
  • Nucleic Acid Sequences Encoding Proteins Associated With Abiotic Stress Response And Plant Cells And Plants With Increased Tolerance To Environmental Stress
  • Nucleic Acid Sequences Encoding Proteins Associated With Abiotic Stress Response And Plant Cells And Plants With In-Creased Tolerance To Environmental Stress
  • Method For Producing Polyunsatured Fatty Acids In Transgenic Plants
  • Protein Phosphatase Stress-Related Polypeptides And Methods Of Use In Plants
  • Process For The Control Of Production Of Fine Chemicals
  • Herbicide-Metabolizing Cytochrome P450 Monooxygenases
  • Method For Producing Arachidonic Acid And/Or Eicosapentaenoic Acid In Useful Transgenic Plants
  • Nucleic Acid Sequences Encoding Proteins Associated With Abiotic Stress Response And Plant Cells And Plants With Increased Tolerance To Environmantal Stress
  • Method For Production Of Polyunsaturated Fatty Acids In Transgenic Organisms
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00272763

    DOI

    http://dx.doi.org/10.1007/bf00272763

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1015345244

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/24240423


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/07", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Agricultural and Veterinary Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0607", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Plant Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0703", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Crop and Pasture Production", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Institut f\u00fcr Genbiologische Forschung Berlin GmbH, Ihnestrasse 63, D-1000, Berlin 33, Germany", 
              "id": "http://www.grid.ac/institutes/grid.418390.7", 
              "name": [
                "Institut f\u00fcr Genbiologische Forschung Berlin GmbH, Ihnestrasse 63, D-1000, Berlin 33, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Schmidt", 
            "givenName": "Renate", 
            "id": "sg:person.01151004272.64", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01151004272.64"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Institut f\u00fcr Genbiologische Forschung Berlin GmbH, Ihnestrasse 63, D-1000, Berlin 33, Germany", 
              "id": "http://www.grid.ac/institutes/grid.418390.7", 
              "name": [
                "Institut f\u00fcr Genbiologische Forschung Berlin GmbH, Ihnestrasse 63, D-1000, Berlin 33, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Willmitzer", 
            "givenName": "Lothar", 
            "id": "sg:person.0102633444.26", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0102633444.26"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1007/bf00269239", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030578233", 
              "https://doi.org/10.1007/bf00269239"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00272531", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022519223", 
              "https://doi.org/10.1007/bf00272531"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00021308", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009001455", 
              "https://doi.org/10.1007/bf00021308"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00044244", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000128661", 
              "https://doi.org/10.1007/bf00044244"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00325695", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013879592", 
              "https://doi.org/10.1007/bf00325695"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00330254", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011873691", 
              "https://doi.org/10.1007/bf00330254"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00383491", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036108700", 
              "https://doi.org/10.1007/bf00383491"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00269251", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009953882", 
              "https://doi.org/10.1007/bf00269251"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "1988-12", 
        "datePublishedReg": "1988-12-01", 
        "description": "A highly efficient and fast Agrobacterium-mediated leaf disc transformation system for the Arabidopsis thaliana L. genotype C24 was developed. This protocol is also amenable to other ecotypes - as could be shown for Landsberg erecta and Wassllewskija. Besides the hygromycin selection also the G418 and kanamycin selection were established. Furthermore the described procedure is appliable not only to leaf explants but also to expanded cotyledons which proved to be an excellent alternative as explant source for transformation experiments.", 
        "genre": "article", 
        "id": "sg:pub.10.1007/bf00272763", 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1124809", 
            "issn": [
              "0721-7714", 
              "1432-203X"
            ], 
            "name": "Plant Cell Reports", 
            "publisher": "Springer Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "7", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "7"
          }
        ], 
        "keywords": [
          "Agrobacterium tumefaciens-mediated transformation", 
          "kanamycin selection", 
          "hygromycin selection", 
          "transformation system", 
          "transformation experiments", 
          "cotyledon explants", 
          "excellent alternative", 
          "leaf explants", 
          "explant source", 
          "protocol", 
          "G418", 
          "explants", 
          "alternative", 
          "system", 
          "selection", 
          "thaliana leaves", 
          "transformation", 
          "source", 
          "experiments", 
          "procedure", 
          "C24", 
          "Landsberg erecta", 
          "cotyledons", 
          "leaves", 
          "Arabidopsis thaliana leaves", 
          "erecta", 
          "ecotypes"
        ], 
        "name": "High efficiency Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana leaf and cotyledon explants", 
        "pagination": "583-586", 
        "productId": [
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1015345244"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/bf00272763"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "24240423"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/bf00272763", 
          "https://app.dimensions.ai/details/publication/pub.1015345244"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2022-09-02T15:46", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-springernature-scigraph/baseset/20220902/entities/gbq_results/article/article_201.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://doi.org/10.1007/bf00272763"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00272763'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00272763'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00272763'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00272763'


     

    This table displays all metadata directly associated to this object as RDF triples.

    135 TRIPLES      21 PREDICATES      63 URIs      45 LITERALS      7 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/bf00272763 schema:about anzsrc-for:06
    2 anzsrc-for:0607
    3 anzsrc-for:07
    4 anzsrc-for:0703
    5 schema:author N885a5714cdba433999027cf94caa85cd
    6 schema:citation sg:pub.10.1007/bf00021308
    7 sg:pub.10.1007/bf00044244
    8 sg:pub.10.1007/bf00269239
    9 sg:pub.10.1007/bf00269251
    10 sg:pub.10.1007/bf00272531
    11 sg:pub.10.1007/bf00325695
    12 sg:pub.10.1007/bf00330254
    13 sg:pub.10.1007/bf00383491
    14 schema:datePublished 1988-12
    15 schema:datePublishedReg 1988-12-01
    16 schema:description A highly efficient and fast Agrobacterium-mediated leaf disc transformation system for the Arabidopsis thaliana L. genotype C24 was developed. This protocol is also amenable to other ecotypes - as could be shown for Landsberg erecta and Wassllewskija. Besides the hygromycin selection also the G418 and kanamycin selection were established. Furthermore the described procedure is appliable not only to leaf explants but also to expanded cotyledons which proved to be an excellent alternative as explant source for transformation experiments.
    17 schema:genre article
    18 schema:isAccessibleForFree false
    19 schema:isPartOf N1e5720b032fd4cb3a1eece70a978a63d
    20 Nea0c444523064824a7d97fecbcf40d17
    21 sg:journal.1124809
    22 schema:keywords Agrobacterium tumefaciens-mediated transformation
    23 Arabidopsis thaliana leaves
    24 C24
    25 G418
    26 Landsberg erecta
    27 alternative
    28 cotyledon explants
    29 cotyledons
    30 ecotypes
    31 erecta
    32 excellent alternative
    33 experiments
    34 explant source
    35 explants
    36 hygromycin selection
    37 kanamycin selection
    38 leaf explants
    39 leaves
    40 procedure
    41 protocol
    42 selection
    43 source
    44 system
    45 thaliana leaves
    46 transformation
    47 transformation experiments
    48 transformation system
    49 schema:name High efficiency Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana leaf and cotyledon explants
    50 schema:pagination 583-586
    51 schema:productId N17b4383c6d5b482fbeb607aea4acab00
    52 Nb222a657560c46fe84bca63995b0317f
    53 Nb880880d07504640968f6ef2cf5d271d
    54 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015345244
    55 https://doi.org/10.1007/bf00272763
    56 schema:sdDatePublished 2022-09-02T15:46
    57 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    58 schema:sdPublisher Nc786392539fd4ba8821ecc2b7ebdbfdd
    59 schema:url https://doi.org/10.1007/bf00272763
    60 sgo:license sg:explorer/license/
    61 sgo:sdDataset articles
    62 rdf:type schema:ScholarlyArticle
    63 N17b4383c6d5b482fbeb607aea4acab00 schema:name dimensions_id
    64 schema:value pub.1015345244
    65 rdf:type schema:PropertyValue
    66 N1e5720b032fd4cb3a1eece70a978a63d schema:issueNumber 7
    67 rdf:type schema:PublicationIssue
    68 N885a5714cdba433999027cf94caa85cd rdf:first sg:person.01151004272.64
    69 rdf:rest Nd3c1d239b11d4652a63898d6a10584fe
    70 Nb222a657560c46fe84bca63995b0317f schema:name pubmed_id
    71 schema:value 24240423
    72 rdf:type schema:PropertyValue
    73 Nb880880d07504640968f6ef2cf5d271d schema:name doi
    74 schema:value 10.1007/bf00272763
    75 rdf:type schema:PropertyValue
    76 Nc786392539fd4ba8821ecc2b7ebdbfdd schema:name Springer Nature - SN SciGraph project
    77 rdf:type schema:Organization
    78 Nd3c1d239b11d4652a63898d6a10584fe rdf:first sg:person.0102633444.26
    79 rdf:rest rdf:nil
    80 Nea0c444523064824a7d97fecbcf40d17 schema:volumeNumber 7
    81 rdf:type schema:PublicationVolume
    82 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    83 schema:name Biological Sciences
    84 rdf:type schema:DefinedTerm
    85 anzsrc-for:0607 schema:inDefinedTermSet anzsrc-for:
    86 schema:name Plant Biology
    87 rdf:type schema:DefinedTerm
    88 anzsrc-for:07 schema:inDefinedTermSet anzsrc-for:
    89 schema:name Agricultural and Veterinary Sciences
    90 rdf:type schema:DefinedTerm
    91 anzsrc-for:0703 schema:inDefinedTermSet anzsrc-for:
    92 schema:name Crop and Pasture Production
    93 rdf:type schema:DefinedTerm
    94 sg:journal.1124809 schema:issn 0721-7714
    95 1432-203X
    96 schema:name Plant Cell Reports
    97 schema:publisher Springer Nature
    98 rdf:type schema:Periodical
    99 sg:person.0102633444.26 schema:affiliation grid-institutes:grid.418390.7
    100 schema:familyName Willmitzer
    101 schema:givenName Lothar
    102 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0102633444.26
    103 rdf:type schema:Person
    104 sg:person.01151004272.64 schema:affiliation grid-institutes:grid.418390.7
    105 schema:familyName Schmidt
    106 schema:givenName Renate
    107 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01151004272.64
    108 rdf:type schema:Person
    109 sg:pub.10.1007/bf00021308 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009001455
    110 https://doi.org/10.1007/bf00021308
    111 rdf:type schema:CreativeWork
    112 sg:pub.10.1007/bf00044244 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000128661
    113 https://doi.org/10.1007/bf00044244
    114 rdf:type schema:CreativeWork
    115 sg:pub.10.1007/bf00269239 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030578233
    116 https://doi.org/10.1007/bf00269239
    117 rdf:type schema:CreativeWork
    118 sg:pub.10.1007/bf00269251 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009953882
    119 https://doi.org/10.1007/bf00269251
    120 rdf:type schema:CreativeWork
    121 sg:pub.10.1007/bf00272531 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022519223
    122 https://doi.org/10.1007/bf00272531
    123 rdf:type schema:CreativeWork
    124 sg:pub.10.1007/bf00325695 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013879592
    125 https://doi.org/10.1007/bf00325695
    126 rdf:type schema:CreativeWork
    127 sg:pub.10.1007/bf00330254 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011873691
    128 https://doi.org/10.1007/bf00330254
    129 rdf:type schema:CreativeWork
    130 sg:pub.10.1007/bf00383491 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036108700
    131 https://doi.org/10.1007/bf00383491
    132 rdf:type schema:CreativeWork
    133 grid-institutes:grid.418390.7 schema:alternateName Institut für Genbiologische Forschung Berlin GmbH, Ihnestrasse 63, D-1000, Berlin 33, Germany
    134 schema:name Institut für Genbiologische Forschung Berlin GmbH, Ihnestrasse 63, D-1000, Berlin 33, Germany
    135 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...