Improved protoplast culture and regeneration of shoots in pea (Pisum sativum L.) View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1988-06

AUTHORS

Johanna Puonti-Kaerlas, Tage Eriksson

ABSTRACT

An improved system involving a modification of the bead culture system was developed for culturing pea protoplasts. Using this method, sustained divisions and callus growth could be obtained in all 10 cultivars tested. In the best responding cultivar division frequency could be raised from 17% in liquid culture to 80% in the bead system. Shoot regeneration with a reproducible frequency of about 1% could be obtained from protoplast-derived calli in two of the tested cultivars. More... »

PAGES

242-245

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00272533

DOI

http://dx.doi.org/10.1007/bf00272533

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1050525587

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24241757


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