Stable transformation of moth bean Vigna aconitifolia via direct gene transfer View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1987-07

AUTHORS

F. Köhler, C. Golz, S. Eapen, H. Kohn, O. Schieder

ABSTRACT

Direct gene transfer proved to be an efficient transformation method for Vigna aconitifolia, a member of the legume family. Kanamycin resistant calli and plants were regenerated from heat shocked protoplasts treated with PEG and plasmid DNA containing the coding region for aminoglycoside phosphotransferase gene (NPT II). The plant cultivar used was an important factor in attaining higher transformation frequencies. Transformation was confirmed by Southern blot analysis using a non-radioactive detection system. Attempts to transform mesophyll and suspension cultured cells by this method were unsuccessful. Protoplasts electroporated with the plasmid pCAP212, which codes for chloramphenicol acetyltransferase, exhibited transient expression of this gene two days after treatment while electroporated cells did not show this enzyme activity. It is therefore assumed that the DNA uptake is prevented by the cell wall. More... »

PAGES

313-317

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00272007

DOI

http://dx.doi.org/10.1007/bf00272007

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1015952758

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24248768


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