Ontology type: schema:ScholarlyArticle
1987-07
AUTHORSW. H. Chen, K. M. A. Gartland, M. R. Davey, R. Sotak, J. S. Gartland, B. J. Mulligan, J. B. Power, E. C. Cocking
ABSTRACTSugarcane protoplasts were transformed to kanamycin resistance at a frequency of approximately 8 in 10(7) following PEG-induced uptake of Sma1 linearised pABD1 plasmid. DNA-treated protoplasts were cultured in agarose droplets, and protoplast-derived transformants selected on 80 μg ml(-1) kanamycin. Transformed tissues maintained on this level of antibiotic expressed APH(3')II activity, and contained DNA that hybridised to a probe with the APH(3')II gene. More... »
PAGES297-301
http://scigraph.springernature.com/pub.10.1007/bf00272003
DOIhttp://dx.doi.org/10.1007/bf00272003
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PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/24248764
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