Transformation of sugarcane protoplasts by direct uptake of a selectable chimaeric gene View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1987-07

AUTHORS

W. H. Chen, K. M. A. Gartland, M. R. Davey, R. Sotak, J. S. Gartland, B. J. Mulligan, J. B. Power, E. C. Cocking

ABSTRACT

Sugarcane protoplasts were transformed to kanamycin resistance at a frequency of approximately 8 in 10(7) following PEG-induced uptake of Sma1 linearised pABD1 plasmid. DNA-treated protoplasts were cultured in agarose droplets, and protoplast-derived transformants selected on 80 μg ml(-1) kanamycin. Transformed tissues maintained on this level of antibiotic expressed APH(3')II activity, and contained DNA that hybridised to a probe with the APH(3')II gene. More... »

PAGES

297-301

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00272003

DOI

http://dx.doi.org/10.1007/bf00272003

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1034768431

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24248764


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