Ontology type: schema:ScholarlyArticle
1981-11
AUTHORSV. Buchanan-Wollaston, M. C. Cannon, F. C. Cannon
ABSTRACTSome restriction endonuclease fragments of nif DNA, when carried on small multicopy plasmids, inhibited nif expression in Klebsiella pneumoniae. A study of this inhibitory effect revealed, (1) that overproduction of the nifL gene product inhibited transcription of two nif operons examined, nifJ and nifHDKY and, (2) that when transcription was initiated from the promoter of the nifHDKY operon on multicopy plasmids there was a corresponding decrease in the transcription rates of the chromosomally located nifJ and nifHDKY but not the nifLA operon. Studies of transcription in vivo also showed that the nifA gene product was essential for transcription initiation from the nifHDKY and nifBQ promoters. These results, taken with earlier observations (see Discussion) provide evidence that the nifL and nifA gene products are respectively a repressor and activator of nif transcription initiation from all nif promoters except that of the nifLA operon. More... »
PAGES102-106
http://scigraph.springernature.com/pub.10.1007/bf00271203
DOIhttp://dx.doi.org/10.1007/bf00271203
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1051554253
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/6278243
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Genetics",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cloning, Molecular",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "DNA Restriction Enzymes",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "DNA, Bacterial",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Klebsiella pneumoniae",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Nitrogen Fixation",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Operon",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Phenotype",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Transcription, Genetic",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "A.R.C. Unit of Nitrogen Fixation, University of Sussex, BN1 9RQ, Brighton, UK",
"id": "http://www.grid.ac/institutes/grid.12082.39",
"name": [
"A.R.C. Unit of Nitrogen Fixation, University of Sussex, BN1 9RQ, Brighton, UK"
],
"type": "Organization"
},
"familyName": "Buchanan-Wollaston",
"givenName": "V.",
"id": "sg:person.0601241361.53",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0601241361.53"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "A.R.C. Unit of Nitrogen Fixation, University of Sussex, BN1 9RQ, Brighton, UK",
"id": "http://www.grid.ac/institutes/grid.12082.39",
"name": [
"A.R.C. Unit of Nitrogen Fixation, University of Sussex, BN1 9RQ, Brighton, UK"
],
"type": "Organization"
},
"familyName": "Cannon",
"givenName": "M. C.",
"id": "sg:person.01047105170.87",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01047105170.87"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "A.R.C. Unit of Nitrogen Fixation, University of Sussex, BN1 9RQ, Brighton, UK",
"id": "http://www.grid.ac/institutes/grid.12082.39",
"name": [
"A.R.C. Unit of Nitrogen Fixation, University of Sussex, BN1 9RQ, Brighton, UK"
],
"type": "Organization"
},
"familyName": "Cannon",
"givenName": "F. C.",
"id": "sg:person.01345730553.18",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01345730553.18"
],
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1007/978-3-642-67919-3_23",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1012584496",
"https://doi.org/10.1007/978-3-642-67919-3_23"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00433306",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1046705218",
"https://doi.org/10.1007/bf00433306"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/290424a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1035620095",
"https://doi.org/10.1038/290424a0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/286128a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1015962314",
"https://doi.org/10.1038/286128a0"
],
"type": "CreativeWork"
}
],
"datePublished": "1981-11",
"datePublishedReg": "1981-11-01",
"description": "Some restriction endonuclease fragments of nif DNA, when carried on small multicopy plasmids, inhibited nif expression in Klebsiella pneumoniae. A study of this inhibitory effect revealed, (1) that overproduction of the nifL gene product inhibited transcription of two nif operons examined, nifJ and nifHDKY and, (2) that when transcription was initiated from the promoter of the nifHDKY operon on multicopy plasmids there was a corresponding decrease in the transcription rates of the chromosomally located nifJ and nifHDKY but not the nifLA operon. Studies of transcription in vivo also showed that the nifA gene product was essential for transcription initiation from the nifHDKY and nifBQ promoters. These results, taken with earlier observations (see Discussion) provide evidence that the nifL and nifA gene products are respectively a repressor and activator of nif transcription initiation from all nif promoters except that of the nifLA operon.",
"genre": "article",
"id": "sg:pub.10.1007/bf00271203",
"isAccessibleForFree": false,
"isPartOf": [
{
"id": "sg:journal.1297380",
"issn": [
"1617-4615",
"1432-1874"
],
"name": "Molecular Genetics and Genomics",
"publisher": "Springer Nature",
"type": "Periodical"
},
{
"issueNumber": "1",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "184"
}
],
"keywords": [
"Klebsiella pneumoniae",
"inhibitory effect",
"gene products",
"pneumoniae",
"expression",
"corresponding decrease",
"initiation",
"earlier observations",
"transcription rate",
"vivo",
"study",
"transcription",
"promoter",
"transcriptional regulation",
"restriction endonuclease fragments",
"plasmid",
"nif DNA",
"overproduction",
"activator",
"DNA",
"small multicopy plasmids",
"nif expression",
"evidence",
"endonuclease fragments",
"nifHDKY operon",
"decrease",
"regulation",
"rate",
"effect",
"use",
"nifLA operon",
"fragments",
"nifA gene product",
"products",
"results",
"repressor",
"observations",
"multicopy plasmid",
"study of transcription",
"transcription initiation",
"operon",
"nifL gene product",
"nifJ",
"nif promoters",
"nif operons",
"NifL"
],
"name": "The use of cloned nif (Nitrogen fixation) DNA to investigate transcriptional regulation of nif expression in Klebsiella pneumoniae",
"pagination": "102-106",
"productId": [
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1051554253"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1007/bf00271203"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"6278243"
]
}
],
"sameAs": [
"https://doi.org/10.1007/bf00271203",
"https://app.dimensions.ai/details/publication/pub.1051554253"
],
"sdDataset": "articles",
"sdDatePublished": "2022-08-04T16:50",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-springernature-scigraph/baseset/20220804/entities/gbq_results/article/article_173.jsonl",
"type": "ScholarlyArticle",
"url": "https://doi.org/10.1007/bf00271203"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00271203'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00271203'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00271203'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00271203'
This table displays all metadata directly associated to this object as RDF triples.
169 TRIPLES
21 PREDICATES
84 URIs
72 LITERALS
15 BLANK NODES