Somatic hybridisation of plants by fusion of protoplasts View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1974-12

AUTHORS

Georg Melchers, Gudrun Labib

ABSTRACT

Mesophyll protoplasts of “haploids” produced by “anther culture” of two chlorophyll deficient, light sensitive varieties of Nicotiana tabacum, sublethal (s) and virescent (v) are fused with the method of Keller and Melchers (1973). The double heterozygote hybrid of the two recessive genes for light sensitivity complements to normal leaf colour and resistance to high light intensity. By using this complementation obviously 12, possibly 21 independently arisen somatic hybrid plants are selected. Since leaf colour mutations are frequent and light sensitivity of leaf colour mutants is not too rare in higher plants, it is possible to use generally this selection method. More... »

PAGES

277-294

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Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00271144

DOI

http://dx.doi.org/10.1007/bf00271144

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1049230604


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