Immunological detection of nitric oxide synthase(s) in human tissues using heterologous antibodies suggesting different isoforms View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1992-11

AUTHORS

D. R. Springall, V. Riveros-Moreno, L. Buttery, A. Suburo, A. E. Bishop, M. Merrett, S. Moncada, J. M. Polak

ABSTRACT

Nitric oxide (NO) is generated from l-arginine by NO synthases. Localization of the brain enzyme has been carried out in the rat; however, despite data suggesting that NO is a major regulator of vascular and neural functions in man, there is no information about the localization of NO synthase in human tissues. Rabbit antisera to NO synthase purified from rat brain (antisera A and B) were raised, tested by Western blotting, affinity purification and enzyme immunoprecipitation assay, and used to investigate the distribution of the enzyme in a variety of human tissues by immunohistochemistry. Antisera to two synthetic peptides from cloned neural NO synthase were used to aid specificity testing. Antisera A and B reacted with a ∼ 160-kDa protein in Western blots of human brain extracts, gave immunostaining of nerves, and precipitated enzyme activity from rat brain homogenates. Antiserum B to NO synthase also reacted with proteins of Mr between 125 and 140 kDa in extracts of well-vascularised tissues, and immunostained vascular endothelium; the neural and vascular immunoreactivity persisted after affinity purification of antiserum B with the ∼ 160 kDa protein. Endothelial staining with antiserum B was seen in respiratory tract, liver, skin and umbilicus; syncytial trophoblasts stained in the placenta. Neural staining with antiserum A and B was seen in the myenteric and submucous plexus, and in nerve fibres in smooth muscle of the gut and in many areas of the central nervous system, particularly cortex, hippocampus, hypothalamus, cerebellum, brain stem and spinal cord. Therefore, antibodies to rat brain enzyme react with the human equivalent and also with other NO synthase isoforms in human endothelium. These findings support the contention that the endothelial enzyme is a different form with partial homology to that in nerves and also provide an anatomical distribution of NO synthase isoforms. More... »

PAGES

259-266

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00271040

DOI

http://dx.doi.org/10.1007/bf00271040

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1036857784

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/1281146


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1109", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Neurosciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Amino Acid Oxidoreductases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antibodies", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Brain", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Colon", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Immunohistochemistry", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Isoenzymes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Liver", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Lung", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Nitric Oxide Synthase", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Placenta", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Skin", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Trachea", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Department of Histochemistry, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, W12 0NN, London, UK", 
          "id": "http://www.grid.ac/institutes/grid.413629.b", 
          "name": [
            "Department of Histochemistry, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, W12 0NN, London, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Springall", 
        "givenName": "D. R.", 
        "id": "sg:person.01364551234.51", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01364551234.51"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Wellcome Research Laboratories, Langley Court, BR3 3BS, Beckenham, Kent, UK", 
          "id": "http://www.grid.ac/institutes/grid.418236.a", 
          "name": [
            "Wellcome Research Laboratories, Langley Court, BR3 3BS, Beckenham, Kent, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Riveros-Moreno", 
        "givenName": "V.", 
        "id": "sg:person.076171234.44", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.076171234.44"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Histochemistry, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, W12 0NN, London, UK", 
          "id": "http://www.grid.ac/institutes/grid.413629.b", 
          "name": [
            "Department of Histochemistry, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, W12 0NN, London, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Buttery", 
        "givenName": "L.", 
        "id": "sg:person.011101227747.44", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011101227747.44"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Histochemistry, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, W12 0NN, London, UK", 
          "id": "http://www.grid.ac/institutes/grid.413629.b", 
          "name": [
            "Department of Histochemistry, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, W12 0NN, London, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Suburo", 
        "givenName": "A.", 
        "id": "sg:person.0103265613.59", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0103265613.59"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Histochemistry, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, W12 0NN, London, UK", 
          "id": "http://www.grid.ac/institutes/grid.413629.b", 
          "name": [
            "Department of Histochemistry, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, W12 0NN, London, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bishop", 
        "givenName": "A. E.", 
        "id": "sg:person.015254216222.19", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015254216222.19"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Wellcome Research Laboratories, Langley Court, BR3 3BS, Beckenham, Kent, UK", 
          "id": "http://www.grid.ac/institutes/grid.418236.a", 
          "name": [
            "Wellcome Research Laboratories, Langley Court, BR3 3BS, Beckenham, Kent, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Merrett", 
        "givenName": "M.", 
        "id": "sg:person.0610377460.19", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0610377460.19"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Wellcome Research Laboratories, Langley Court, BR3 3BS, Beckenham, Kent, UK", 
          "id": "http://www.grid.ac/institutes/grid.418236.a", 
          "name": [
            "Wellcome Research Laboratories, Langley Court, BR3 3BS, Beckenham, Kent, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Moncada", 
        "givenName": "S.", 
        "id": "sg:person.0642263312.40", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0642263312.40"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Histochemistry, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, W12 0NN, London, UK", 
          "id": "http://www.grid.ac/institutes/grid.413629.b", 
          "name": [
            "Department of Histochemistry, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, W12 0NN, London, UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Polak", 
        "givenName": "J. M.", 
        "id": "sg:person.0670441457.36", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0670441457.36"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/347768a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005544862", 
          "https://doi.org/10.1038/347768a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/327524a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025944311", 
          "https://doi.org/10.1038/327524a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/327324a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053083816", 
          "https://doi.org/10.1038/327324a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/351714a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008241995", 
          "https://doi.org/10.1038/351714a0"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1992-11", 
    "datePublishedReg": "1992-11-01", 
    "description": "Nitric oxide (NO) is generated from l-arginine by NO synthases. Localization of the brain enzyme has been carried out in the rat; however, despite data suggesting that NO is a major regulator of vascular and neural functions in man, there is no information about the localization of NO synthase in human tissues. Rabbit antisera to NO synthase purified from rat brain (antisera A and B) were raised, tested by Western blotting, affinity purification and enzyme immunoprecipitation assay, and used to investigate the distribution of the enzyme in a variety of human tissues by immunohistochemistry. Antisera to two synthetic peptides from cloned neural NO synthase were used to aid specificity testing. Antisera A and B reacted with a \u223c 160-kDa protein in Western blots of human brain extracts, gave immunostaining of nerves, and precipitated enzyme activity from rat brain homogenates. Antiserum B to NO synthase also reacted with proteins of Mr between 125 and 140 kDa in extracts of well-vascularised tissues, and immunostained vascular endothelium; the neural and vascular immunoreactivity persisted after affinity purification of antiserum B with the \u223c 160 kDa protein. Endothelial staining with antiserum B was seen in respiratory tract, liver, skin and umbilicus; syncytial trophoblasts stained in the placenta. Neural staining with antiserum A and B was seen in the myenteric and submucous plexus, and in nerve fibres in smooth muscle of the gut and in many areas of the central nervous system, particularly cortex, hippocampus, hypothalamus, cerebellum, brain stem and spinal cord. Therefore, antibodies to rat brain enzyme react with the human equivalent and also with other NO synthase isoforms in human endothelium. These findings support the contention that the endothelial enzyme is a different form with partial homology to that in nerves and also provide an anatomical distribution of NO synthase isoforms.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/bf00271040", 
    "inLanguage": "en", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1294817", 
        "issn": [
          "0948-6143", 
          "1432-119X"
        ], 
        "name": "Histochemistry and Cell Biology", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "4", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "98"
      }
    ], 
    "keywords": [
      "nitric oxide", 
      "synthase isoforms", 
      "neural NO synthase", 
      "central nervous system", 
      "human brain extracts", 
      "human tissues", 
      "rat brain homogenates", 
      "vascular immunoreactivity", 
      "antiserum A", 
      "submucous plexus", 
      "brain stem", 
      "spinal cord", 
      "respiratory tract", 
      "nerve fibers", 
      "NO synthase", 
      "endothelial staining", 
      "smooth muscle", 
      "anatomical distribution", 
      "rat brain", 
      "vascular endothelium", 
      "nervous system", 
      "brain homogenates", 
      "syncytial trophoblast", 
      "endothelial enzymes", 
      "brain extracts", 
      "neural function", 
      "Western blot", 
      "human endothelium", 
      "heterologous antibodies", 
      "Western blotting", 
      "antiserum B", 
      "brain enzyme", 
      "nerve", 
      "human equivalent", 
      "endothelium", 
      "tissue", 
      "antibodies", 
      "major regulator", 
      "synthase", 
      "synthetic peptides", 
      "isoforms", 
      "different isoforms", 
      "immunological detection", 
      "hippocampus", 
      "enzyme activity", 
      "plexus", 
      "cord", 
      "rats", 
      "umbilicus", 
      "immunohistochemistry", 
      "immunoreactivity", 
      "cerebellum", 
      "immunostaining", 
      "placenta", 
      "tract", 
      "trophoblast", 
      "brain", 
      "protein of Mr", 
      "liver", 
      "muscle", 
      "rabbits", 
      "protein", 
      "men", 
      "staining", 
      "skin", 
      "blotting", 
      "specificity testing", 
      "gut", 
      "blot", 
      "extracts", 
      "homogenates", 
      "enzyme", 
      "arginine", 
      "localization", 
      "MR", 
      "findings", 
      "peptides", 
      "affinity purification", 
      "immunoprecipitation", 
      "testing", 
      "partial homology", 
      "activity", 
      "different forms", 
      "regulator", 
      "function", 
      "data", 
      "fibers", 
      "detection", 
      "area", 
      "stem", 
      "oxide", 
      "synthases", 
      "equivalent", 
      "variety", 
      "information", 
      "homology", 
      "form", 
      "distribution", 
      "purification", 
      "contention", 
      "system", 
      "reacts", 
      "enzyme reacts", 
      "enzyme immunoprecipitation", 
      "immunostaining of nerves", 
      "brain enzyme react"
    ], 
    "name": "Immunological detection of nitric oxide synthase(s) in human tissues using heterologous antibodies suggesting different isoforms", 
    "pagination": "259-266", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1036857784"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/bf00271040"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "1281146"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/bf00271040", 
      "https://app.dimensions.ai/details/publication/pub.1036857784"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-01-01T18:06", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220101/entities/gbq_results/article/article_253.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/bf00271040"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00271040'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00271040'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00271040'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00271040'


 

This table displays all metadata directly associated to this object as RDF triples.

288 TRIPLES      22 PREDICATES      150 URIs      138 LITERALS      20 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/bf00271040 schema:about N059154fd724f49318886507b84835616
2 N1d17703cb37a4b31822b0c56d52df9fc
3 N1d35d5246ee24ff58a4f987cd67a84bd
4 N1e00e24556d64b2d83974f911375e8ef
5 N2ee495243d8f4bab845595c12db5fde4
6 N3b5549fefa154130a90b1df31fe1975b
7 N4f16d3a1e8a14ae08d0850146b544a96
8 N5d46df00142245e7a4a0dd28e173ea8e
9 N749f2ce4ed334cfca4f4a8740d3bf1d4
10 N884fceceffe742e680f45aecd3db068d
11 Nc5395f0954bd4a9280811669ad22b5a4
12 Ndd842b7ae8a347c7aa7ed7f657dfbce8
13 Neccabc4680e549fc8b29f9e2b83f298e
14 anzsrc-for:11
15 anzsrc-for:1109
16 schema:author Nf3fd46fdb9344d0192bb9e9d3be63402
17 schema:citation sg:pub.10.1038/327324a0
18 sg:pub.10.1038/327524a0
19 sg:pub.10.1038/347768a0
20 sg:pub.10.1038/351714a0
21 schema:datePublished 1992-11
22 schema:datePublishedReg 1992-11-01
23 schema:description Nitric oxide (NO) is generated from l-arginine by NO synthases. Localization of the brain enzyme has been carried out in the rat; however, despite data suggesting that NO is a major regulator of vascular and neural functions in man, there is no information about the localization of NO synthase in human tissues. Rabbit antisera to NO synthase purified from rat brain (antisera A and B) were raised, tested by Western blotting, affinity purification and enzyme immunoprecipitation assay, and used to investigate the distribution of the enzyme in a variety of human tissues by immunohistochemistry. Antisera to two synthetic peptides from cloned neural NO synthase were used to aid specificity testing. Antisera A and B reacted with a ∼ 160-kDa protein in Western blots of human brain extracts, gave immunostaining of nerves, and precipitated enzyme activity from rat brain homogenates. Antiserum B to NO synthase also reacted with proteins of Mr between 125 and 140 kDa in extracts of well-vascularised tissues, and immunostained vascular endothelium; the neural and vascular immunoreactivity persisted after affinity purification of antiserum B with the ∼ 160 kDa protein. Endothelial staining with antiserum B was seen in respiratory tract, liver, skin and umbilicus; syncytial trophoblasts stained in the placenta. Neural staining with antiserum A and B was seen in the myenteric and submucous plexus, and in nerve fibres in smooth muscle of the gut and in many areas of the central nervous system, particularly cortex, hippocampus, hypothalamus, cerebellum, brain stem and spinal cord. Therefore, antibodies to rat brain enzyme react with the human equivalent and also with other NO synthase isoforms in human endothelium. These findings support the contention that the endothelial enzyme is a different form with partial homology to that in nerves and also provide an anatomical distribution of NO synthase isoforms.
24 schema:genre article
25 schema:inLanguage en
26 schema:isAccessibleForFree false
27 schema:isPartOf N0bd3e08a461a459bb29c765f7ae22e9d
28 N98396986ae50485b8717ef6338c74b3a
29 sg:journal.1294817
30 schema:keywords MR
31 NO synthase
32 Western blot
33 Western blotting
34 activity
35 affinity purification
36 anatomical distribution
37 antibodies
38 antiserum A
39 antiserum B
40 area
41 arginine
42 blot
43 blotting
44 brain
45 brain enzyme
46 brain enzyme react
47 brain extracts
48 brain homogenates
49 brain stem
50 central nervous system
51 cerebellum
52 contention
53 cord
54 data
55 detection
56 different forms
57 different isoforms
58 distribution
59 endothelial enzymes
60 endothelial staining
61 endothelium
62 enzyme
63 enzyme activity
64 enzyme immunoprecipitation
65 enzyme reacts
66 equivalent
67 extracts
68 fibers
69 findings
70 form
71 function
72 gut
73 heterologous antibodies
74 hippocampus
75 homogenates
76 homology
77 human brain extracts
78 human endothelium
79 human equivalent
80 human tissues
81 immunohistochemistry
82 immunological detection
83 immunoprecipitation
84 immunoreactivity
85 immunostaining
86 immunostaining of nerves
87 information
88 isoforms
89 liver
90 localization
91 major regulator
92 men
93 muscle
94 nerve
95 nerve fibers
96 nervous system
97 neural NO synthase
98 neural function
99 nitric oxide
100 oxide
101 partial homology
102 peptides
103 placenta
104 plexus
105 protein
106 protein of Mr
107 purification
108 rabbits
109 rat brain
110 rat brain homogenates
111 rats
112 reacts
113 regulator
114 respiratory tract
115 skin
116 smooth muscle
117 specificity testing
118 spinal cord
119 staining
120 stem
121 submucous plexus
122 syncytial trophoblast
123 synthase
124 synthase isoforms
125 synthases
126 synthetic peptides
127 system
128 testing
129 tissue
130 tract
131 trophoblast
132 umbilicus
133 variety
134 vascular endothelium
135 vascular immunoreactivity
136 schema:name Immunological detection of nitric oxide synthase(s) in human tissues using heterologous antibodies suggesting different isoforms
137 schema:pagination 259-266
138 schema:productId N22412c90899748649c0336c8708276fa
139 N4ed1eeca1d3f44978c78a5db16553cf3
140 Nb307832784404be7957b11bf1b81a1e2
141 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036857784
142 https://doi.org/10.1007/bf00271040
143 schema:sdDatePublished 2022-01-01T18:06
144 schema:sdLicense https://scigraph.springernature.com/explorer/license/
145 schema:sdPublisher Ne59c475816e64ddcb9dbb85c4c6cbc4c
146 schema:url https://doi.org/10.1007/bf00271040
147 sgo:license sg:explorer/license/
148 sgo:sdDataset articles
149 rdf:type schema:ScholarlyArticle
150 N059154fd724f49318886507b84835616 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
151 schema:name Nitric Oxide Synthase
152 rdf:type schema:DefinedTerm
153 N0bd3e08a461a459bb29c765f7ae22e9d schema:volumeNumber 98
154 rdf:type schema:PublicationVolume
155 N1d17703cb37a4b31822b0c56d52df9fc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
156 schema:name Antibodies
157 rdf:type schema:DefinedTerm
158 N1d35d5246ee24ff58a4f987cd67a84bd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
159 schema:name Immunohistochemistry
160 rdf:type schema:DefinedTerm
161 N1e00e24556d64b2d83974f911375e8ef schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
162 schema:name Lung
163 rdf:type schema:DefinedTerm
164 N22412c90899748649c0336c8708276fa schema:name dimensions_id
165 schema:value pub.1036857784
166 rdf:type schema:PropertyValue
167 N2ee495243d8f4bab845595c12db5fde4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
168 schema:name Trachea
169 rdf:type schema:DefinedTerm
170 N3b5549fefa154130a90b1df31fe1975b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
171 schema:name Colon
172 rdf:type schema:DefinedTerm
173 N4973e97613014631ac773d8b1f268a8c rdf:first sg:person.0610377460.19
174 rdf:rest Nb40e65e7e37c4accb2199fcbb67af3f1
175 N4ed1eeca1d3f44978c78a5db16553cf3 schema:name pubmed_id
176 schema:value 1281146
177 rdf:type schema:PropertyValue
178 N4f16d3a1e8a14ae08d0850146b544a96 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
179 schema:name Amino Acid Oxidoreductases
180 rdf:type schema:DefinedTerm
181 N5d46df00142245e7a4a0dd28e173ea8e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
182 schema:name Brain
183 rdf:type schema:DefinedTerm
184 N6ce8600f220c468f9ce24fb8298cd9ba rdf:first sg:person.015254216222.19
185 rdf:rest N4973e97613014631ac773d8b1f268a8c
186 N70d13cd0155a4854be36196b8b497950 rdf:first sg:person.0103265613.59
187 rdf:rest N6ce8600f220c468f9ce24fb8298cd9ba
188 N749f2ce4ed334cfca4f4a8740d3bf1d4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
189 schema:name Placenta
190 rdf:type schema:DefinedTerm
191 N884fceceffe742e680f45aecd3db068d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
192 schema:name Skin
193 rdf:type schema:DefinedTerm
194 N98396986ae50485b8717ef6338c74b3a schema:issueNumber 4
195 rdf:type schema:PublicationIssue
196 Nab53033266e34058992273b08357b3d1 rdf:first sg:person.011101227747.44
197 rdf:rest N70d13cd0155a4854be36196b8b497950
198 Nb307832784404be7957b11bf1b81a1e2 schema:name doi
199 schema:value 10.1007/bf00271040
200 rdf:type schema:PropertyValue
201 Nb40e65e7e37c4accb2199fcbb67af3f1 rdf:first sg:person.0642263312.40
202 rdf:rest Nfad3e52138f14faeac26670158d5eacf
203 Nbe110926bc784a55a13ae7d7430c022b rdf:first sg:person.076171234.44
204 rdf:rest Nab53033266e34058992273b08357b3d1
205 Nc5395f0954bd4a9280811669ad22b5a4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
206 schema:name Humans
207 rdf:type schema:DefinedTerm
208 Ndd842b7ae8a347c7aa7ed7f657dfbce8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
209 schema:name Liver
210 rdf:type schema:DefinedTerm
211 Ne59c475816e64ddcb9dbb85c4c6cbc4c schema:name Springer Nature - SN SciGraph project
212 rdf:type schema:Organization
213 Neccabc4680e549fc8b29f9e2b83f298e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
214 schema:name Isoenzymes
215 rdf:type schema:DefinedTerm
216 Nf3fd46fdb9344d0192bb9e9d3be63402 rdf:first sg:person.01364551234.51
217 rdf:rest Nbe110926bc784a55a13ae7d7430c022b
218 Nfad3e52138f14faeac26670158d5eacf rdf:first sg:person.0670441457.36
219 rdf:rest rdf:nil
220 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
221 schema:name Medical and Health Sciences
222 rdf:type schema:DefinedTerm
223 anzsrc-for:1109 schema:inDefinedTermSet anzsrc-for:
224 schema:name Neurosciences
225 rdf:type schema:DefinedTerm
226 sg:journal.1294817 schema:issn 0948-6143
227 1432-119X
228 schema:name Histochemistry and Cell Biology
229 schema:publisher Springer Nature
230 rdf:type schema:Periodical
231 sg:person.0103265613.59 schema:affiliation grid-institutes:grid.413629.b
232 schema:familyName Suburo
233 schema:givenName A.
234 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0103265613.59
235 rdf:type schema:Person
236 sg:person.011101227747.44 schema:affiliation grid-institutes:grid.413629.b
237 schema:familyName Buttery
238 schema:givenName L.
239 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011101227747.44
240 rdf:type schema:Person
241 sg:person.01364551234.51 schema:affiliation grid-institutes:grid.413629.b
242 schema:familyName Springall
243 schema:givenName D. R.
244 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01364551234.51
245 rdf:type schema:Person
246 sg:person.015254216222.19 schema:affiliation grid-institutes:grid.413629.b
247 schema:familyName Bishop
248 schema:givenName A. E.
249 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015254216222.19
250 rdf:type schema:Person
251 sg:person.0610377460.19 schema:affiliation grid-institutes:grid.418236.a
252 schema:familyName Merrett
253 schema:givenName M.
254 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0610377460.19
255 rdf:type schema:Person
256 sg:person.0642263312.40 schema:affiliation grid-institutes:grid.418236.a
257 schema:familyName Moncada
258 schema:givenName S.
259 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0642263312.40
260 rdf:type schema:Person
261 sg:person.0670441457.36 schema:affiliation grid-institutes:grid.413629.b
262 schema:familyName Polak
263 schema:givenName J. M.
264 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0670441457.36
265 rdf:type schema:Person
266 sg:person.076171234.44 schema:affiliation grid-institutes:grid.418236.a
267 schema:familyName Riveros-Moreno
268 schema:givenName V.
269 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.076171234.44
270 rdf:type schema:Person
271 sg:pub.10.1038/327324a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053083816
272 https://doi.org/10.1038/327324a0
273 rdf:type schema:CreativeWork
274 sg:pub.10.1038/327524a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025944311
275 https://doi.org/10.1038/327524a0
276 rdf:type schema:CreativeWork
277 sg:pub.10.1038/347768a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005544862
278 https://doi.org/10.1038/347768a0
279 rdf:type schema:CreativeWork
280 sg:pub.10.1038/351714a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008241995
281 https://doi.org/10.1038/351714a0
282 rdf:type schema:CreativeWork
283 grid-institutes:grid.413629.b schema:alternateName Department of Histochemistry, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, W12 0NN, London, UK
284 schema:name Department of Histochemistry, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, W12 0NN, London, UK
285 rdf:type schema:Organization
286 grid-institutes:grid.418236.a schema:alternateName Wellcome Research Laboratories, Langley Court, BR3 3BS, Beckenham, Kent, UK
287 schema:name Wellcome Research Laboratories, Langley Court, BR3 3BS, Beckenham, Kent, UK
288 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...