Transport of l(-)malic acid and other dicarboxylic acids in the yeast Candida sphaerica View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1989-10

AUTHORS

Manuela Côrte-Real, C. Leão, N. van Uden

ABSTRACT

dl-Malic acid grown cells of Candida sphaerica (anamorph of Kluyveromyces marxianus) formed a saturable transport system that mediated accumulative transport of l(-)malic acid with the following kinetic parameters at pH 5.0: Vmax, 0.44 nmol l(-)malate·s-1 per milligram dry weight; Km,0.1 mMl(-)malate. Initial uptake of the acid was accompanied by disappearance of extracellular protons, the rates of which followed Michaelis-Menten kinetics as a function of the acid concentration. Variation with extracellular pH of the Kmvalues, calculated either as the concentrations of anions or of undissociated acid, pointed to anions as the transported form. Furthermore, accumulated free acid suffered rapid efflux after the addition of the protonophore carbonylcyanide-M-chlorophenyl-hydrazone (CCCP). These results suggested that the transport system was a dicarboxylate-proton symporter. The system was inducible and was subject to glucose repression. Succinic, fumaric, α-ketoglutaric, oxaloacetic and d-malic acid, but not maleic, malonic, oxalic nor l(+)-tartaric acid, apparently used the same transport system since they acted as competitive inhibitors of l(-)malic acid transport and induced proton movements that followed Michaelis-Menten kinetics. Experiments with glucose-repressed cells showed that undissociated dicarboxylic acid (measured with labelled succinic acid) entered the cells slowly by simple diffusion. The permeability of the cells for undissociated acid increased exponentially with pH, the diffusion constant increasing 100-fold between pH 3.5 and 6.0. More... »

PAGES

551-555

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00270793

DOI

http://dx.doi.org/10.1007/bf00270793

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1033864913


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of Minho", 
          "id": "https://www.grid.ac/institutes/grid.10328.38", 
          "name": [
            "Laboratory of Biology, University of Minho, 4719, Braga Codex, Portugal"
          ], 
          "type": "Organization"
        }, 
        "familyName": "C\u00f4rte-Real", 
        "givenName": "Manuela", 
        "id": "sg:person.01255066520.52", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01255066520.52"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Minho", 
          "id": "https://www.grid.ac/institutes/grid.10328.38", 
          "name": [
            "Laboratory of Biology, University of Minho, 4719, Braga Codex, Portugal"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Le\u00e3o", 
        "givenName": "C.", 
        "id": "sg:person.012250306174.21", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012250306174.21"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Instituto Gulbenkian de Ci\u00eancia", 
          "id": "https://www.grid.ac/institutes/grid.418346.c", 
          "name": [
            "Laboratory of Microbiology, Gulbenkian Institute of Science, 2781, Oeiras Codex, Portugal"
          ], 
          "type": "Organization"
        }, 
        "familyName": "van Uden", 
        "givenName": "N.", 
        "id": "sg:person.07571423510.66", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07571423510.66"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/bf00257039", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005925377", 
          "https://doi.org/10.1007/bf00257039"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00257039", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005925377", 
          "https://doi.org/10.1007/bf00257039"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0005-2736(84)90272-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005981119"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0005-2736(84)90272-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005981119"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1139/m75-066", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010547415"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00394646", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014391452", 
          "https://doi.org/10.1007/bf00394646"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00394646", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014391452", 
          "https://doi.org/10.1007/bf00394646"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01941765", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041300727", 
          "https://doi.org/10.1007/bf01941765"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01941765", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041300727", 
          "https://doi.org/10.1007/bf01941765"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0076-6879(79)55066-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042351986"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00406676", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050831322", 
          "https://doi.org/10.1007/bf00406676"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00406676", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050831322", 
          "https://doi.org/10.1007/bf00406676"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1099/00221287-23-1-65", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060371890"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.168.3.1439-1443.1986", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062715384"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079516919", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082108805", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1989-10", 
    "datePublishedReg": "1989-10-01", 
    "description": "dl-Malic acid grown cells of Candida sphaerica (anamorph of Kluyveromyces marxianus) formed a saturable transport system that mediated accumulative transport of l(-)malic acid with the following kinetic parameters at pH 5.0: Vmax, 0.44 nmol l(-)malate\u00b7s-1 per milligram dry weight; Km,0.1 mMl(-)malate. Initial uptake of the acid was accompanied by disappearance of extracellular protons, the rates of which followed Michaelis-Menten kinetics as a function of the acid concentration. Variation with extracellular pH of the Kmvalues, calculated either as the concentrations of anions or of undissociated acid, pointed to anions as the transported form. Furthermore, accumulated free acid suffered rapid efflux after the addition of the protonophore carbonylcyanide-M-chlorophenyl-hydrazone (CCCP). These results suggested that the transport system was a dicarboxylate-proton symporter. The system was inducible and was subject to glucose repression. Succinic, fumaric, \u03b1-ketoglutaric, oxaloacetic and d-malic acid, but not maleic, malonic, oxalic nor l(+)-tartaric acid, apparently used the same transport system since they acted as competitive inhibitors of l(-)malic acid transport and induced proton movements that followed Michaelis-Menten kinetics. Experiments with glucose-repressed cells showed that undissociated dicarboxylic acid (measured with labelled succinic acid) entered the cells slowly by simple diffusion. The permeability of the cells for undissociated acid increased exponentially with pH, the diffusion constant increasing 100-fold between pH 3.5 and 6.0.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/bf00270793", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1083533", 
        "issn": [
          "0175-7598", 
          "1432-0614"
        ], 
        "name": "Applied Microbiology and Biotechnology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "5-6", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "31"
      }
    ], 
    "name": "Transport of l(-)malic acid and other dicarboxylic acids in the yeast Candida sphaerica", 
    "pagination": "551-555", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "a152cf11b5f6f9221200b71b94542dc6a79834845a38d67529845528f2589c9b"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/bf00270793"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1033864913"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/bf00270793", 
      "https://app.dimensions.ai/details/publication/pub.1033864913"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T13:59", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000371_0000000371/records_130826_00000003.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007/BF00270793"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00270793'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00270793'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00270793'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00270793'


 

This table displays all metadata directly associated to this object as RDF triples.

113 TRIPLES      21 PREDICATES      38 URIs      19 LITERALS      7 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/bf00270793 schema:about anzsrc-for:06
2 anzsrc-for:0601
3 schema:author N047fd2ed7567429bab2a7aece2a21a84
4 schema:citation sg:pub.10.1007/bf00257039
5 sg:pub.10.1007/bf00394646
6 sg:pub.10.1007/bf00406676
7 sg:pub.10.1007/bf01941765
8 https://app.dimensions.ai/details/publication/pub.1079516919
9 https://app.dimensions.ai/details/publication/pub.1082108805
10 https://doi.org/10.1016/0005-2736(84)90272-4
11 https://doi.org/10.1016/0076-6879(79)55066-6
12 https://doi.org/10.1099/00221287-23-1-65
13 https://doi.org/10.1128/jb.168.3.1439-1443.1986
14 https://doi.org/10.1139/m75-066
15 schema:datePublished 1989-10
16 schema:datePublishedReg 1989-10-01
17 schema:description dl-Malic acid grown cells of Candida sphaerica (anamorph of Kluyveromyces marxianus) formed a saturable transport system that mediated accumulative transport of l(-)malic acid with the following kinetic parameters at pH 5.0: Vmax, 0.44 nmol l(-)malate·s-1 per milligram dry weight; Km,0.1 mMl(-)malate. Initial uptake of the acid was accompanied by disappearance of extracellular protons, the rates of which followed Michaelis-Menten kinetics as a function of the acid concentration. Variation with extracellular pH of the Kmvalues, calculated either as the concentrations of anions or of undissociated acid, pointed to anions as the transported form. Furthermore, accumulated free acid suffered rapid efflux after the addition of the protonophore carbonylcyanide-M-chlorophenyl-hydrazone (CCCP). These results suggested that the transport system was a dicarboxylate-proton symporter. The system was inducible and was subject to glucose repression. Succinic, fumaric, α-ketoglutaric, oxaloacetic and d-malic acid, but not maleic, malonic, oxalic nor l(+)-tartaric acid, apparently used the same transport system since they acted as competitive inhibitors of l(-)malic acid transport and induced proton movements that followed Michaelis-Menten kinetics. Experiments with glucose-repressed cells showed that undissociated dicarboxylic acid (measured with labelled succinic acid) entered the cells slowly by simple diffusion. The permeability of the cells for undissociated acid increased exponentially with pH, the diffusion constant increasing 100-fold between pH 3.5 and 6.0.
18 schema:genre research_article
19 schema:inLanguage en
20 schema:isAccessibleForFree false
21 schema:isPartOf N0d3d0b79e9b34c018ee4d3d71fbb16c4
22 N89446095ef2346469dd54c398c6048b2
23 sg:journal.1083533
24 schema:name Transport of l(-)malic acid and other dicarboxylic acids in the yeast Candida sphaerica
25 schema:pagination 551-555
26 schema:productId N61984ea295944e6381928a48aef08988
27 N92421e4b26834f39864ab885042708c8
28 Naa0232432c2e4496becbd03cbeebc826
29 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033864913
30 https://doi.org/10.1007/bf00270793
31 schema:sdDatePublished 2019-04-11T13:59
32 schema:sdLicense https://scigraph.springernature.com/explorer/license/
33 schema:sdPublisher N22a6039e3c0d4bd086b4e3570955d59c
34 schema:url http://link.springer.com/10.1007/BF00270793
35 sgo:license sg:explorer/license/
36 sgo:sdDataset articles
37 rdf:type schema:ScholarlyArticle
38 N047fd2ed7567429bab2a7aece2a21a84 rdf:first sg:person.01255066520.52
39 rdf:rest Nb98c5aab388b43b2b26f6af49e051f67
40 N0d3d0b79e9b34c018ee4d3d71fbb16c4 schema:issueNumber 5-6
41 rdf:type schema:PublicationIssue
42 N22a6039e3c0d4bd086b4e3570955d59c schema:name Springer Nature - SN SciGraph project
43 rdf:type schema:Organization
44 N61984ea295944e6381928a48aef08988 schema:name dimensions_id
45 schema:value pub.1033864913
46 rdf:type schema:PropertyValue
47 N89446095ef2346469dd54c398c6048b2 schema:volumeNumber 31
48 rdf:type schema:PublicationVolume
49 N92421e4b26834f39864ab885042708c8 schema:name doi
50 schema:value 10.1007/bf00270793
51 rdf:type schema:PropertyValue
52 Naa0232432c2e4496becbd03cbeebc826 schema:name readcube_id
53 schema:value a152cf11b5f6f9221200b71b94542dc6a79834845a38d67529845528f2589c9b
54 rdf:type schema:PropertyValue
55 Nb98c5aab388b43b2b26f6af49e051f67 rdf:first sg:person.012250306174.21
56 rdf:rest Nd8335e992cae4c7abfd14d277737d99c
57 Nd8335e992cae4c7abfd14d277737d99c rdf:first sg:person.07571423510.66
58 rdf:rest rdf:nil
59 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
60 schema:name Biological Sciences
61 rdf:type schema:DefinedTerm
62 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
63 schema:name Biochemistry and Cell Biology
64 rdf:type schema:DefinedTerm
65 sg:journal.1083533 schema:issn 0175-7598
66 1432-0614
67 schema:name Applied Microbiology and Biotechnology
68 rdf:type schema:Periodical
69 sg:person.012250306174.21 schema:affiliation https://www.grid.ac/institutes/grid.10328.38
70 schema:familyName Leão
71 schema:givenName C.
72 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012250306174.21
73 rdf:type schema:Person
74 sg:person.01255066520.52 schema:affiliation https://www.grid.ac/institutes/grid.10328.38
75 schema:familyName Côrte-Real
76 schema:givenName Manuela
77 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01255066520.52
78 rdf:type schema:Person
79 sg:person.07571423510.66 schema:affiliation https://www.grid.ac/institutes/grid.418346.c
80 schema:familyName van Uden
81 schema:givenName N.
82 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07571423510.66
83 rdf:type schema:Person
84 sg:pub.10.1007/bf00257039 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005925377
85 https://doi.org/10.1007/bf00257039
86 rdf:type schema:CreativeWork
87 sg:pub.10.1007/bf00394646 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014391452
88 https://doi.org/10.1007/bf00394646
89 rdf:type schema:CreativeWork
90 sg:pub.10.1007/bf00406676 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050831322
91 https://doi.org/10.1007/bf00406676
92 rdf:type schema:CreativeWork
93 sg:pub.10.1007/bf01941765 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041300727
94 https://doi.org/10.1007/bf01941765
95 rdf:type schema:CreativeWork
96 https://app.dimensions.ai/details/publication/pub.1079516919 schema:CreativeWork
97 https://app.dimensions.ai/details/publication/pub.1082108805 schema:CreativeWork
98 https://doi.org/10.1016/0005-2736(84)90272-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005981119
99 rdf:type schema:CreativeWork
100 https://doi.org/10.1016/0076-6879(79)55066-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042351986
101 rdf:type schema:CreativeWork
102 https://doi.org/10.1099/00221287-23-1-65 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060371890
103 rdf:type schema:CreativeWork
104 https://doi.org/10.1128/jb.168.3.1439-1443.1986 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062715384
105 rdf:type schema:CreativeWork
106 https://doi.org/10.1139/m75-066 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010547415
107 rdf:type schema:CreativeWork
108 https://www.grid.ac/institutes/grid.10328.38 schema:alternateName University of Minho
109 schema:name Laboratory of Biology, University of Minho, 4719, Braga Codex, Portugal
110 rdf:type schema:Organization
111 https://www.grid.ac/institutes/grid.418346.c schema:alternateName Instituto Gulbenkian de Ciência
112 schema:name Laboratory of Microbiology, Gulbenkian Institute of Science, 2781, Oeiras Codex, Portugal
113 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...