Survival of cultured cells and somatic embryos of Asparagus officinalis cryopreserved by vitrification View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1989-10

AUTHORS

A. Uragami, A. Sakai, M. Nagai, T. Takahashi

ABSTRACT

Cultured cells and somatic embryos derived from the mesophyll tissue of asparagus (Asparagus officinalis L.) were cryopreserved by vitrification. The vitrification solution (PVS) contains (w/v) 22% glycerol, 15% ethylene glycol, 15% propylene glycol and 7% DMSO in Murashige-Skoog medium enriched with 0.5M sorbitol. After initial cryoprotection with sorbitol supplemented MS medium containing 12% ethylene glycol, cells or embryos were exposed stepwise to 85% PVS at 0°C. They were loaded into 0.5 ml transparent straws, and were then plunged directly into liquid nitrogen. After rapid warming, PVS was removed and diluted stepwise. The highest survivals of vitrified cells and embryos were about 65 and 50%, respectively. Surviving embryos developed into plantlets. More... »

PAGES

418-421

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00270083

DOI

http://dx.doi.org/10.1007/bf00270083

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1028635486

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24233367


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