Transformation of cotton (Gossypium hirsutum L.) via particle bombardment View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1990-03

AUTHORS

John J. Finer, Michael D. McMullen

ABSTRACT

Embryogenic suspension cultures of cotton (Gossypium hirsutum L.) were subjected to particle bombardment, where high density particles carrying plasmid DNA were accelerated towards the embryogenic plant cells. The plasmid DNA coating the particles encoded hygromycin resistance. One to two weeks following bombardment, embryogenic cotton cells were placed in proliferation medium containing 100 μg/ml hygromycin. Clumps of tissue which grew in the presence of hygromycin were subcultured at low density into fresh hygromycin-containing proliferation medium. Following sequential transfer of embryogenic tissue to development and then germination media, plants were recovered from transgenic embryogenic tissue. Southern hybridization confirmed the presence of the hygromycin resistance gene in embryogenic suspension culture tissue and regenerated plants. More... »

PAGES

586-589

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00270059

DOI

http://dx.doi.org/10.1007/bf00270059

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1052558109

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24232677


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144 Department of Agronomy and Ohio State Biotechnology Center, Ohio Agricultural Research and Development Center, The Ohio State University, 44691, Wooster, OH, USA
145 rdf:type schema:Organization
 




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