Agrobacterium — and microprojectile — mediated viral DNA delivery into barley microspore-derived cultures View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1990-04

AUTHORS

Gary Creissen, Caroline Smith, Russell Francis, Helen Reynolds, Phil Mullineaux

ABSTRACT

Anther cultures of barley (Hordeum vulgare L. var. "Igri") were used as targets for Agrobacterium-mediated DNA transfer and direct DNA uptake by particle bombardment. A wheat dwarf virus construct which can replicate to a high copy number in cereal cells provided a sensitive marker for successful DNA delivery. Although DNA delivery was achieved using both procedures, particle bombardment gave more reproducible and higher levels of infection. The ability to deliver DNA into cereal cells which have a high regeneration capacity may provide a route for stable transformation. More... »

PAGES

680-683

Journal

TITLE

Plant Cell Reports

ISSUE

11

VOLUME

8

Author Affiliations

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00269992

    DOI

    http://dx.doi.org/10.1007/bf00269992

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1043482696

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/24232785


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