Plant regeneration from protoplasts of soybean (Glycine max L.) View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1988-08

AUTHORS

Zhi-ming Wei, Zhi-hong Xu

ABSTRACT

Protoplasts were isolated from immature cotyledons of six cultivars of Glycine max L. and cultured in the KP8 liquid medium supplemented with 0.2 mg/L 2,4-D, 1 mg/L NAA and 0.5 mg/L ZT. The protoplasts started to divide after 3-5 days of culture. Sustained divisions resulted in mass production of cell colonies and small calli in 6 weeks. The calli further grew to 2-3 mm on the gelritesolidified K8 medium and were transferred onto the MSB medium with 1 mg/L 2,4-D and 0.25 mg/L BA, to obtain compact and nodular calli. Shoot formation was initiated on MSB medium with 0.15 mg/L NAA, and BA, KT and ZT, 0.5 mg/L of each, with or without 500 mg/L CH. It was followed by plant regeneration. So far, 87 plants have been regenerated from 4 cultivars, and normal seeds were obtained from them after transplanting into pots. More... »

PAGES

348-351

Journal

TITLE

Plant Cell Reports

ISSUE

5

VOLUME

7

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00269935

DOI

http://dx.doi.org/10.1007/bf00269935

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1034453565

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24241881


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