Ontology type: schema:ScholarlyArticle
1988-08
AUTHORSW. H. Chen, M. R. Davey, J. B. Power, E. C. Cocking
ABSTRACTSugarcane cell suspensions were initiated from leaf callus and sub-cultured every 7 to 10 days by alternate transfer to MS based medium with 3.0 and 1.0 mg 1(-1)2,4-D. Suspensions older than 3 months gave the most reproducible yields of protoplasts. Isolated protoplasts required 50 mM Ca(2+) in the washing solution and 100 mM Ca(2+) in the culture medium to prevent lysis. At plating densities of 2.0-3.0×10(5) ml(-1), 18% or more of the isolated protoplasts produced cell colonies when cultured in droplets or sectors of Kao and Michayluk (1975) based medium with 1.2% w/v Sea Plaque agarose. Cell colonies were of two morphological types. Those consisting of small, tightly packed cells developed into morphogenic callus. The latter produced an abundance of green meristems from which shoots and whole plants were regenerated. More... »
PAGES344-347
http://scigraph.springernature.com/pub.10.1007/bf00269934
DOIhttp://dx.doi.org/10.1007/bf00269934
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1006656713
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/24241880
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0607",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Plant Biology",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"name": [
"Plant Genetic Manipulation Group, Department of Botany, University of Nottingham, NG7 2RD, University Park, Nottingham, U.K."
],
"type": "Organization"
},
"familyName": "Chen",
"givenName": "W. H.",
"id": "sg:person.01350715337.68",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01350715337.68"
],
"type": "Person"
},
{
"affiliation": {
"name": [
"Plant Genetic Manipulation Group, Department of Botany, University of Nottingham, NG7 2RD, University Park, Nottingham, U.K."
],
"type": "Organization"
},
"familyName": "Davey",
"givenName": "M. R.",
"id": "sg:person.01264611513.23",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01264611513.23"
],
"type": "Person"
},
{
"affiliation": {
"name": [
"Plant Genetic Manipulation Group, Department of Botany, University of Nottingham, NG7 2RD, University Park, Nottingham, U.K."
],
"type": "Organization"
},
"familyName": "Power",
"givenName": "J. B.",
"id": "sg:person.01150363113.94",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01150363113.94"
],
"type": "Person"
},
{
"affiliation": {
"name": [
"Plant Genetic Manipulation Group, Department of Botany, University of Nottingham, NG7 2RD, University Park, Nottingham, U.K."
],
"type": "Organization"
},
"familyName": "Cocking",
"givenName": "E. C.",
"id": "sg:person.012433432757.93",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012433432757.93"
],
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1007/bf02318913",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1000630447",
"https://doi.org/10.1007/bf02318913"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf02318913",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1000630447",
"https://doi.org/10.1007/bf02318913"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0168-9452(86)90059-2",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1001053334"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00033560",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1001290974",
"https://doi.org/10.1007/bf00033560"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00033560",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1001290974",
"https://doi.org/10.1007/bf00033560"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0012-1606(73)90169-3",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1002894965"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.5511/plantbiotechnology1984.2.74",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1004606501"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00380613",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1006514198",
"https://doi.org/10.1007/bf00380613"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00380613",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1006514198",
"https://doi.org/10.1007/bf00380613"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0176-1617(87)80234-1",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1007014702"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0044-328x(83)80082-8",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1007908174"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00269240",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1009523328",
"https://doi.org/10.1007/bf00269240"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00269240",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1009523328",
"https://doi.org/10.1007/bf00269240"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0304-4211(84)90250-5",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1014152287"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0304-4211(84)90250-5",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1014152287"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0044-328x(84)80034-3",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1017391334"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/b978-0-12-715001-7.50052-4",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1019275663"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00265079",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1020966020",
"https://doi.org/10.1007/bf00265079"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00265079",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1020966020",
"https://doi.org/10.1007/bf00265079"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1111/j.1399-3054.1962.tb08052.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1023703429"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0044-328x(81)80070-0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1025921856"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00387684",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1030364187",
"https://doi.org/10.1007/bf00387684"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00387684",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1030364187",
"https://doi.org/10.1007/bf00387684"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/978-3-642-66646-9_9",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1030392248",
"https://doi.org/10.1007/978-3-642-66646-9_9"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0168-9452(85)90164-5",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1030767517"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00268120",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1036028356",
"https://doi.org/10.1007/bf00268120"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00268120",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1036028356",
"https://doi.org/10.1007/bf00268120"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1111/j.1399-3054.1976.tb03934.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1036281808"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0044-328x(83)80092-0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1036549063"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/978-94-009-2788-9_12",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1039876338",
"https://doi.org/10.1007/978-94-009-2788-9_12"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00269151",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1040500001",
"https://doi.org/10.1007/bf00269151"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00269151",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1040500001",
"https://doi.org/10.1007/bf00269151"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00393231",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1042699957",
"https://doi.org/10.1007/bf00393231"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00393231",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1042699957",
"https://doi.org/10.1007/bf00393231"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0176-1617(86)80214-0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1053085632"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1139/b82-269",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1053181016"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/jxb/39.2.251",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1059856588"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1126/science.198.4315.405",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1062515954"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/oxfordjournals.pcp.a075553",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1083626646"
],
"type": "CreativeWork"
}
],
"datePublished": "1988-08",
"datePublishedReg": "1988-08-01",
"description": "Sugarcane cell suspensions were initiated from leaf callus and sub-cultured every 7 to 10 days by alternate transfer to MS based medium with 3.0 and 1.0 mg 1(-1)2,4-D. Suspensions older than 3 months gave the most reproducible yields of protoplasts. Isolated protoplasts required 50 mM Ca(2+) in the washing solution and 100 mM Ca(2+) in the culture medium to prevent lysis. At plating densities of 2.0-3.0\u00d710(5) ml(-1), 18% or more of the isolated protoplasts produced cell colonies when cultured in droplets or sectors of Kao and Michayluk (1975) based medium with 1.2% w/v Sea Plaque agarose. Cell colonies were of two morphological types. Those consisting of small, tightly packed cells developed into morphogenic callus. The latter produced an abundance of green meristems from which shoots and whole plants were regenerated. ",
"genre": "research_article",
"id": "sg:pub.10.1007/bf00269934",
"inLanguage": [
"en"
],
"isAccessibleForFree": false,
"isPartOf": [
{
"id": "sg:journal.1124809",
"issn": [
"0721-7714",
"1432-203X"
],
"name": "Plant Cell Reports",
"type": "Periodical"
},
{
"issueNumber": "5",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "7"
}
],
"name": "Sugarcane protoplasts: factors affecting division and plant regeneration",
"pagination": "344-347",
"productId": [
{
"name": "readcube_id",
"type": "PropertyValue",
"value": [
"1fb4f43e58817b54086a0a8ff00ab02f37c3fbc00b6faaa9e603780b2a627bc7"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"24241880"
]
},
{
"name": "nlm_unique_id",
"type": "PropertyValue",
"value": [
"9880970"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1007/bf00269934"
]
},
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1006656713"
]
}
],
"sameAs": [
"https://doi.org/10.1007/bf00269934",
"https://app.dimensions.ai/details/publication/pub.1006656713"
],
"sdDataset": "articles",
"sdDatePublished": "2019-04-11T14:02",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000371_0000000371/records_130831_00000000.jsonl",
"type": "ScholarlyArticle",
"url": "http://link.springer.com/10.1007/BF00269934"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00269934'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00269934'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00269934'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00269934'
This table displays all metadata directly associated to this object as RDF triples.
193 TRIPLES
21 PREDICATES
58 URIs
21 LITERALS
9 BLANK NODES