Sugarcane protoplasts: factors affecting division and plant regeneration View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1988-08

AUTHORS

W. H. Chen, M. R. Davey, J. B. Power, E. C. Cocking

ABSTRACT

Sugarcane cell suspensions were initiated from leaf callus and sub-cultured every 7 to 10 days by alternate transfer to MS based medium with 3.0 and 1.0 mg 1(-1)2,4-D. Suspensions older than 3 months gave the most reproducible yields of protoplasts. Isolated protoplasts required 50 mM Ca(2+) in the washing solution and 100 mM Ca(2+) in the culture medium to prevent lysis. At plating densities of 2.0-3.0×10(5) ml(-1), 18% or more of the isolated protoplasts produced cell colonies when cultured in droplets or sectors of Kao and Michayluk (1975) based medium with 1.2% w/v Sea Plaque agarose. Cell colonies were of two morphological types. Those consisting of small, tightly packed cells developed into morphogenic callus. The latter produced an abundance of green meristems from which shoots and whole plants were regenerated. More... »

PAGES

344-347

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00269934

DOI

http://dx.doi.org/10.1007/bf00269934

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1006656713

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24241880


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