Isolation of protoplasts and regeneration of callus from suspension cultures of cultivated beets View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1985-12

AUTHORS

S. R. Bhat, B. V. Ford-Lloyd, J. A. Callow

ABSTRACT

Conditions necessary for the isolation and culture of protoplasts from suspension cultures of sugar, fodder and garden beets were investigated. Good yields of protoplasts were obtained by treating cells with a mixture of cellulase, Macerozyme and Driselase enzymes. Nutritional requirements of beet protoplasts were found to be quite simple: protoplasts could be cultured in MS, B5 or PGo based media with 0.4 M glucose with the optimum result being produced on KM8p medium. Plating efficiency (P.E) was genotype-dependent with the sugar beet giving better P.E. than the fodder or garden beets used, and higher values being achieved with the use of desalted Driselase for isolation followed by culture on KMBp medium. More... »

PAGES

348-350

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00269896

DOI

http://dx.doi.org/10.1007/bf00269896

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1047809387

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24254080


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