Protoplast culture and plant regeneration from Brassica carinata Braun View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1987-02

AUTHORS

Phan V. Chuong, K. P. Pauls, W. D. Beversdorf

ABSTRACT

Protoplasts isolated from hypocotyls of three-day-old seedlings of Brassica carinata (Braun) cv R-2128 were cultured in a modified Nitsch and Nitsch liquid medium containing 13% sucrose, 0.4% Ficoll, 0.25 mg/l BA, 0.5 mg/l NAA and 0.5 mg/l 2,4-D. The density of medium caused the protoplasts and the developing microcalli to float on the surface of the liquid medium whereas all debris and lysed cells sank to the bottom of the culture plate. After 4-6 weeks developing microcalli were approximately 0.5 mm in diameter and were transferred onto MS medium containing 3% sucrose, 0.4% agarose, 200 mg/l casein hydrolysate, 5 mg/l BA and 0.5 mg/l NAA, pH 5.7. Approximately 20% of the calli transferred to this medium produced plantlets. More... »

PAGES

67-69

Journal

TITLE

Plant Cell Reports

ISSUE

1

VOLUME

6

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00269742

DOI

http://dx.doi.org/10.1007/bf00269742

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1008822422

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24248453


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