Nuclear and polysomal transcripts of T-DNA in octopine crown gall suspension and callus cultures View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1981-07

AUTHORS

Lothar Willmitzer, Leon Otten, Gisela Simons, Wolfgang Schmalenbach, Joachim Schröder, Gudrun Schröder, Marc Van Montagu, Guido de Vos, Jeff Schell

ABSTRACT

To establish a detailed map of the transcribed parts of the T-DNA in two octopine crown gall lines grown in suspension culture, T-DNA-derived steady-state nuclear and polysomal RNA as well as RNA synthesized in isolated nuclei purified from the crown gall tissues, was analyzed by southern blot hybridization to specific fragments of the T-region of the octopine plasmid pTi ACH5. In addition total RNA isolated from the same lines grown as callus tissue on solid agar, was analyzed for T-DNA specific transcripts. The results show that all of the T-DNA is transcribed although different segments are transcribed to significantly different extents. Roughly the same hybridization pattern was found for nuclear and polysomal poly-A+ and poly-A- RNA. The transcription pattern was found to be different for cells in the stationary phase of growth compared with actively growing cells. More... »

PAGES

255-262

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00269667

DOI

http://dx.doi.org/10.1007/bf00269667

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1040181558

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/6945473


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