A protoplast-to-tree system in Microcitrus based on protoplasts derived from a sustained embryogenic callus View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1986-12

AUTHORS

Aliza Vardi, Donald J. Hutchison, Esra Galun

ABSTRACT

Adventitious embryos derived from a zygotic embryo in an in vitro cultured ovule of Microcitrus were transferred several times on solidified medium containing benzyladenine and 3-indoleacetyl-L-alanine to induce embryogenic callus. This callus was maintained for several years on medium devoid of growth regulators without losing its embryogenic capacity. Exposure of this callus to maceration enzymes led to protoplast suspensions. Purified protoplasts were plated in solid medium devoid of growth regulators. Somatic embryos were derived efficiently from individual protoplasts and most of these could be regenerated into mature trees bearing normal flowers and typical fruits. This system differs from the Citrus protoplast-to-tree system. In the latter embryogenic callus was derived from the nucellus of polyembryonic species while Microcitrus is monoembryonic and required hormone-induced callus formation from proliferating zygotic embryos. More... »

PAGES

412-414

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00269629

DOI

http://dx.doi.org/10.1007/bf00269629

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1053520966

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24248393


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