An adaptation of the maxam and gilbert sequencing method to the study of human metaphase chromosomes View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1994-03

AUTHORS

S. F. De Cabo, J. Fernández-Piqueras, G. Visedo

ABSTRACT

We have adapted the chemical DNA sequencing method developed by Maxam and Gilbert to study human metaphase chromosomes. Particularly, the in situ cytosine-specific chemical cleavage has been assayed. After exhaustive treatments, a C-like banding pattern was consistently shown. Since methyl groups interfere with the cytosine-specific chemical cleavage reaction, we assume that heterochromatin remains resistant as a result of the high level of methylation. On the other hand when moderate treatments are applied, T-bands remain as resistant regions, implying that these domains would be the most intensely methylated regions within euchromatin. More... »

PAGES

205-207

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00269545

DOI

http://dx.doi.org/10.1007/bf00269545

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1029326518

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/8056620


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