Embryogenesis following cryopreservation in isolated microspores of rapeseed (Brassica napus L.) View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1988-10

AUTHORS

David G. Charne, Pawel Pukacki, Laima S. Kott, Wallace D. Beversdorf

ABSTRACT

A simple procedure is described for cryopreservation of isolated microspores of rapeseed in liquid nitrogen without loss of embryogenic capacity (i.e. embryogenes is can still be induced following freezing). Microspores frozen in Lichter's (1982) medium with 13% sucrose produced ca. 10% of the embryos yielded by an unfrozen control. Microspores frozen in Lichter's medium with 13% sucrose, and supplemented with 0.5 M glycerol and 0.5 M DMSO produced no embryos. Regeneration of embryos obtained from frozen microspores yielded 88% diploid and 12% haploid plants, while embryos from unfrozen controls produced 7% diploids and 93% haploids. The potential to increase the efficiency of the rapeseed haploidy system using cryopreservation is discussed in light of these results. More... »

PAGES

407-409

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00269524

DOI

http://dx.doi.org/10.1007/bf00269524

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1078814031

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24240256


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