Plant regeneration from protoplasts of embryogenic suspension cultures of orchardgrass (Dactylis glomerata L.) View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1988-10

AUTHORS

Michael E. Horn, Bob V. Conger, Christian T. Harms

ABSTRACT

An embryogenic suspension culture of orchardgrass (Dactylis glomerata L.) consisting of small, embryogenic cell clusters was obtained from callus formed on basal sections of young leaves through a process of selective enrichment. These suspensions were used as a source of protoplasts. The isolated protoplasts divided at a frequency of 0.5-10% when plated in an agarose solidified culture medium. Conditioned medium, in which embryogenic Dactylis suspension cultures had been grown, was found to increase the rate of cell colony formation. Protoplast-derived colonies grew rapidly in a bead-type culture system of floating agarose slabs in liquid medium. New suspension cultures formed as the colonies grew out of the agarose. These cultures were embryogenic and formed green plantlets when plated on a solid medium lacking auxin. The plantlets were established in soil and grown to mature plants. More... »

PAGES

371-374

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00269515

DOI

http://dx.doi.org/10.1007/bf00269515

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1078814022

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24240247


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